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Protein

Chitinase 1

Gene

Cht1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes chitin and may play a role in defense against fungal pathogens containing chitin.1 Publication

Catalytic activityi

Random hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.

GO - Molecular functioni

  • chitinase activity Source: UniProtKB
  • chitin binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism, Chitin degradation, Plant defense, Polysaccharide degradation

Keywords - Ligandi

Chitin-binding

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Names & Taxonomyi

Protein namesi
Recommended name:
Chitinase 1 (EC:3.2.1.14)
Alternative name(s):
Class I chitinase a
Short name:
OsChia1a
Pathogenesis related (PR)-3 chitinase 1
Gene namesi
Name:Cht1
Synonyms:CH16, RC24
Ordered Locus Names:Os06g0726200, LOC_Os06g51060
ORF Names:OsJ_22713, P0017G10.4, P0548E04.24
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladeOryzoideaeOryzeaeOryzinaeOryza
Proteomesi
  • UP000059680 Componenti: Chromosome 6, cultivar: Nipponbare

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence analysisAdd
BLAST
Chaini21 – 323303Chitinase 1PRO_5000139668Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi23 ↔ 38PROSITE-ProRule annotation
Disulfide bondi32 ↔ 44PROSITE-ProRule annotation
Disulfide bondi35 ↔ 63PROSITE-ProRule annotation
Disulfide bondi37 ↔ 51PROSITE-ProRule annotation
Disulfide bondi55 ↔ 59PROSITE-ProRule annotation
Disulfide bondi100 ↔ 162PROSITE-ProRule annotation
Disulfide bondi176 ↔ 184PROSITE-ProRule annotation
Disulfide bondi283 ↔ 315PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ42993.

Expressioni

Tissue specificityi

Expressed in roots, leaves, sheaths and meristems.1 Publication

Inductioni

By fungal elicitor and wounding.1 Publication

Gene expression databases

GenevisibleiQ42993. OS.

Interactioni

Protein-protein interaction databases

STRINGi39947.LOC_Os06g51060.1.

Structurei

3D structure databases

ProteinModelPortaliQ42993.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 6141Chitin-binding type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 chitin-binding type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
InParanoidiQ42993.
OMAiCCSEFGW.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42993-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRALAVVVVA TAFAVVAVRG EQCGSQAGGA LCPNCLCCSQ YGWCGSTSAY
60 70 80 90 100
CGSGCQSQCS GSCGGGGPTP PSGGGGSGVA SIVSRSLFDQ MLLHRNDAAC
110 120 130 140 150
PAKNFYTYDA FVAAANAFPS FATTGDAATR KREVAAFLAQ TSHETTGGWA
160 170 180 190 200
TAPDGPYSWG YCFKEENNGN VGSDYCVQSS QWPCAAGKKY YGRGPIQISY
210 220 230 240 250
NYNYGPAGQA IGSNLLSNPD LVASDATVSF KTAFWFWMTP QSPKPSCHAV
260 270 280 290 300
MTGQWTPNGN DQAAGRVPGY GVVTNIINGG VECGHGADSR VADRIGFYKR
310 320
YCDMLGVSYG ANLDCYNQRP FNS
Length:323
Mass (Da):33,765
Last modified:November 1, 1996 - v1
Checksum:i1C85B6227A33EB06
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16221 Genomic DNA. Translation: BAA03749.1.
EF122477 mRNA. Translation: ABL74564.1.
AP003685 Genomic DNA. Translation: BAD61709.1.
AP004685 Genomic DNA. Translation: BAD61801.1.
AP014962 Genomic DNA. Translation: BAS99596.1.
CM000143 Genomic DNA. Translation: EAZ38338.1.
X56063 mRNA. Translation: CAA39535.1.
PIRiT03614.

Genome annotation databases

EnsemblPlantsiOS06T0726200-02; OS06T0726200-02; OS06G0726200.
GrameneiOS06T0726200-02; OS06T0726200-02; OS06G0726200.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16221 Genomic DNA. Translation: BAA03749.1.
EF122477 mRNA. Translation: ABL74564.1.
AP003685 Genomic DNA. Translation: BAD61709.1.
AP004685 Genomic DNA. Translation: BAD61801.1.
AP014962 Genomic DNA. Translation: BAS99596.1.
CM000143 Genomic DNA. Translation: EAZ38338.1.
X56063 mRNA. Translation: CAA39535.1.
PIRiT03614.

3D structure databases

ProteinModelPortaliQ42993.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi39947.LOC_Os06g51060.1.

Protein family/group databases

CAZyiCBM18. Carbohydrate-Binding Module Family 18.
GH19. Glycoside Hydrolase Family 19.

Proteomic databases

PaxDbiQ42993.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiOS06T0726200-02; OS06T0726200-02; OS06G0726200.
GrameneiOS06T0726200-02; OS06T0726200-02; OS06G0726200.

Phylogenomic databases

eggNOGiKOG4742. Eukaryota.
COG3979. LUCA.
InParanoidiQ42993.
OMAiCCSEFGW.

Gene expression databases

GenevisibleiQ42993. OS.

Family and domain databases

Gene3Di3.30.60.10. 1 hit.
InterProiIPR001002. Chitin-bd_1.
IPR018371. Chitin-binding_1_CS.
IPR016283. Glyco_hydro_19.
IPR000726. Glyco_hydro_19_cat.
IPR023346. Lysozyme-like_dom.
[Graphical view]
PfamiPF00187. Chitin_bind_1. 1 hit.
PF00182. Glyco_hydro_19. 1 hit.
[Graphical view]
PIRSFiPIRSF001060. Endochitinase. 1 hit.
PRINTSiPR00451. CHITINBINDNG.
ProDomiPD000609. Chitin_bd_1. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00270. ChtBD1. 1 hit.
[Graphical view]
SUPFAMiSSF53955. SSF53955. 1 hit.
SSF57016. SSF57016. 1 hit.
PROSITEiPS00026. CHIT_BIND_I_1. 1 hit.
PS50941. CHIT_BIND_I_2. 1 hit.
PS00773. CHITINASE_19_1. 1 hit.
PS00774. CHITINASE_19_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence variation, differential expression and chromosomal location of rice chitinase genes."
    Nishizawa Y., Kishimoto N., Saito A., Hibi T.
    Mol. Gen. Genet. 241:1-10(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. Nipponbare.
  2. "Molecular cloning of chitinase genes in rice seeds."
    Yoon U.H., Kim Y.H.
    Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Ilpoombyeo.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "The genomes of Oryza sativa: a history of duplications."
    Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S., Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.
    , Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J., Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X., Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y., Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L., Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H., Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z., Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L., Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F., Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q., Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J., Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M., McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.
    PLoS Biol. 3:266-281(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  6. "Isolation and characterization of a cDNA for rice chitinase."
    Nishizawa Y., Hibi T.
    Plant Sci. 76:211-218(1991)
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 21-323.
    Strain: cv. Nipponbare.
  7. "Regulation, expression and function of a new basic chitinase gene in rice (Oryza sativa L.)."
    Xu Y., Zhu Q., Panbangred W., Shirasu K., Lamb C.
    Plant Mol. Biol. 30:387-401(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INDUCTION.
  8. "Distribution, structure, organ-specific expression, and phylogenic analysis of the pathogenesis-related protein-3 chitinase gene family in rice (Oryza sativa L.)."
    Nakazaki T., Tsukiyama T., Okumoto Y., Kageyama D., Naito K., Inouye K., Tanisaka T.
    Genome 49:619-630(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHI1_ORYSJ
AccessioniPrimary (citable) accession number: Q42993
Secondary accession number(s): A0A0P0X1A6, Q42996, Q7DM46
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: November 1, 1996
Last modified: May 11, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.