ID   4CL2_ORYSJ              Reviewed;         569 AA.
AC   Q42982; Q0DYF5; Q6YUH6;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   27-SEP-2005, sequence version 2.
DT   24-MAR-2009, entry version 53.
DE   RecName: Full=Probable 4-coumarate--CoA ligase 2;
DE            Short=4CL 2;
DE            Short=Os4CL2;
DE            EC=6.2.1.12;
DE   AltName: Full=4-coumaroyl-CoA synthase 2;
GN   Name=4CL2; Synonyms=4CL.2;
GN   OrderedLocusNames=Os02g0697400, LOC_Os02g46970;
GN   ORFNames=P0666E12.12-1, P0459B01.4-1;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Ehrhartoideae; Oryzeae; Oryza.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Zhao Y., Kung S.D., Bottino P.J.;
RT   "4-coumarate:CoA ligase genes in rice: divergent structure and
RT   differential regulation.";
RL   Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=17210932; DOI=10.1101/gr.5509507;
RG   The rice annotation project (RAP);
RT   "Curated genome annotation of Oryza sativa ssp. japonica and
RT   comparative genome analysis with Arabidopsis thaliana.";
RL   Genome Res. 17:175-183(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   MEDLINE=22752273; PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   GENE FAMILY.
RX   PubMed=18627494; DOI=10.1111/j.1469-8137.2008.02534.x;
RA   de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J.,
RA   Hamberger B., Douglas C.J.;
RT   "Genome-wide analysis of a land plant-specific acyl:coenzyme A
RT   synthetase (ACS) gene family in Arabidopsis, poplar, rice and
RT   Physcomitrella.";
RL   New Phytol. 179:987-1003(2008).
CC   -!- CATALYTIC ACTIVITY: ATP + 4-coumarate + CoA = AMP + diphosphate +
CC       4-coumaroyl-CoA.
CC   -!- PATHWAY: Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene
CC       biosynthesis; 3,4',5-trihydroxystilbene from 4-coumaric acid: step
CC       1/2.
CC   -!- DOMAIN: Both substrate-binding domains (SBD1 and SBD2) are
CC       involved in the substrate recognition, and are sufficient to
CC       confer the substrate specificity.
CC   -!- SIMILARITY: Belongs to the ATP-dependent AMP-binding enzyme
CC       family.
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DR   EMBL; L43362; AAA69580.1; -; mRNA.
DR   EMBL; AP004778; BAD07859.1; -; Genomic_DNA.
DR   EMBL; AP005868; BAD08175.1; -; Genomic_DNA.
DR   EMBL; AP008208; BAF09733.1; -; Genomic_DNA.
DR   EMBL; AK105636; -; NOT_ANNOTATED_CDS; mRNA.
DR   PIR; T03390; T03390.
DR   RefSeq; NP_001047819.1; -.
DR   UniGene; Os.4377; -.
DR   HSSP; P08659; 1LCI.
DR   GeneID; 4330407; -.
DR   KEGG; osa:4330407; -.
DR   Gramene; Q42982; -.
DR   GO; GO:0016207; F:4-coumarate-CoA ligase activity; IEA:EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0009698; P:phenylpropanoid metabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR000873; AMP-dep_Synth/Lig.
DR   Pfam; PF00501; AMP-binding; 1.
DR   PROSITE; PS00455; AMP_BINDING; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Ligase; Nucleotide-binding; Phenylpropanoid metabolism.
FT   CHAIN         1    569       Probable 4-coumarate--CoA ligase 2.
FT                                /FTId=PRO_0000193032.
FT   NP_BIND     219    227       ATP (By similarity).
FT   NP_BIND     362    367       ATP (By similarity).
FT   REGION      292    361       SBD1.
FT   REGION      362    429       SBD2.
FT   BINDING     450    450       ATP (By similarity).
FT   BINDING     465    465       ATP (By similarity).
FT   BINDING     556    556       ATP (By similarity).
FT   CONFLICT    138    138       A -> G (in Ref. 1; AAA69580).
FT   CONFLICT    186    187       EG -> KA (in Ref. 1; AAA69580).
FT   CONFLICT    226    226       P -> A (in Ref. 1; AAA69580).
FT   CONFLICT    242    243       QQ -> HE (in Ref. 1; AAA69580).
FT   CONFLICT    277    283       CAVRAGA -> SRVRPAP (in Ref. 1; AAA69580).
FT   CONFLICT    308    308       A -> G (in Ref. 1; AAA69580).
FT   CONFLICT    316    316       L -> V (in Ref. 1; AAA69580).
FT   CONFLICT    450    450       D -> N (in Ref. 1; AAA69580).
FT   CONFLICT    493    493       A -> R (in Ref. 1; AAA69580).
SQ   SEQUENCE   569 AA;  60815 MW;  2A1A1FF3CF7FEC91 CRC64;
     MITVAAPEAQ PQVAAAVDEA PPEAVTVFRS KLPDIDIPSH LPLHEYCFAR AAELPDAPCL
     IAAATGRTYT FAETRLLCRR AAAALHRLGV GHGDRVMVLL QNCVEFAVAF FAASFLGAVT
     TAANPFCTPQ EIHKQFKASG VKLILTQSVY VDKLRQHEAF PRIDACTVGD DTLTVITIDD
     DEATPEGCLP FWDLIADADE GSVPEVAISP DDPVALPFSS GTTGLPKGVV LTHRSVVSGV
     AQQVDGENPN LHMGAGDVAL CVLPLFHIFS LNSVLLCAVR AGAAVALMPR FEMGAMLGAI
     ERWRVTVAAV VPPLVLALAK NPFVERHDLS SIRIVLSGAA PLGKELEDAL RARLPQAIFG
     QGYGMTEAGP VLSMCPAFAK EPTPAKSGSC GTVVRNAELK VVDPDTGFSL GRNLPGEICI
     RGPQIMKGYL NDPEATAATI DVEGWLHTGD IGYVDDDDEV FIVDRVKELI KFKGFQVPPA
     ELESLLIAHP SIADAAVVPQ KDDVAGEVPV AFVVRAADSD ITEESIKEFI SKQVVFYKRL
     HKVHFIHAIP KSASGKILRR ELRAKLAAC
//