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Reviewed, UniProtKB/Swiss-Prot Q42982 (4CL2_ORYSJ)

Last modified September 22, 2009. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Probable 4-coumarate--CoA ligase 2
      Short name=4CL 2
      Short name=Os4CL2
    EC=6.2.1.12
Alternative name(s):
    4-coumaroyl-CoA synthase 2
Gene names
Name: 4CL2
Synonyms: 4CL.2
Ordered Locus Names: Os02g0697400, LOC_Os02g46970
ORF Names: P0666E12.12-1, P0459B01.4-1
OrganismOryza sativa subsp. japonica (Rice)
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

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Protein attributes

Sequence length569 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Catalytic activity

ATP + 4-coumarate + CoA = AMP + diphosphate + 4-coumaroyl-CoA.

Pathway

Phytoalexin biosynthesis; 3,4',5-trihydroxystilbene biosynthesis; 3,4',5-trihydroxystilbene from trans-4-coumarate: step 1/2.

Domain

Both substrate-binding domains (SBD1 and SBD2) are involved in the substrate recognition, and are sufficient to confer the substrate specificity.

Sequence similarities

Belongs to the ATP-dependent AMP-binding enzyme family.

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Ontologies

Keywords
   Biological processPhenylpropanoid metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological processphenylpropanoid metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular function4-coumarate-CoA ligase activity

Inferred from electronic annotation. Source: EC

ATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 569569Probable 4-coumarate--CoA ligase 2
PRO_0000193032

Regions

Nucleotide binding219 – 2279ATP By similarity
Nucleotide binding362 – 3676ATP By similarity
Region292 – 36170SBD1
Region362 – 42968SBD2

Sites

Binding site4501ATP By similarity
Binding site4651ATP By similarity
Binding site5561ATP By similarity

Experimental info

Sequence conflict1381A → G in AAA69580. Ref.1
Sequence conflict186 – 1872EG → KA in AAA69580. Ref.1
Sequence conflict2261P → A in AAA69580. Ref.1
Sequence conflict242 – 2432QQ → HE in AAA69580. Ref.1
Sequence conflict277 – 2837CAVRAGA → SRVRPAP in AAA69580. Ref.1
Sequence conflict3081A → G in AAA69580. Ref.1
Sequence conflict3161L → V in AAA69580. Ref.1
Sequence conflict4501D → N in AAA69580. Ref.1
Sequence conflict4931A → R in AAA69580. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
Q42982-1 [UniParc].

Last modified September 27, 2005. Version 2.
Checksum: 2A1A1FF3CF7FEC91

FASTA56960,815
        10         20         30         40         50         60 
MITVAAPEAQ PQVAAAVDEA PPEAVTVFRS KLPDIDIPSH LPLHEYCFAR AAELPDAPCL 

        70         80         90        100        110        120 
IAAATGRTYT FAETRLLCRR AAAALHRLGV GHGDRVMVLL QNCVEFAVAF FAASFLGAVT 

       130        140        150        160        170        180 
TAANPFCTPQ EIHKQFKASG VKLILTQSVY VDKLRQHEAF PRIDACTVGD DTLTVITIDD 

       190        200        210        220        230        240 
DEATPEGCLP FWDLIADADE GSVPEVAISP DDPVALPFSS GTTGLPKGVV LTHRSVVSGV 

       250        260        270        280        290        300 
AQQVDGENPN LHMGAGDVAL CVLPLFHIFS LNSVLLCAVR AGAAVALMPR FEMGAMLGAI 

       310        320        330        340        350        360 
ERWRVTVAAV VPPLVLALAK NPFVERHDLS SIRIVLSGAA PLGKELEDAL RARLPQAIFG 

       370        380        390        400        410        420 
QGYGMTEAGP VLSMCPAFAK EPTPAKSGSC GTVVRNAELK VVDPDTGFSL GRNLPGEICI 

       430        440        450        460        470        480 
RGPQIMKGYL NDPEATAATI DVEGWLHTGD IGYVDDDDEV FIVDRVKELI KFKGFQVPPA 

       490        500        510        520        530        540 
ELESLLIAHP SIADAAVVPQ KDDVAGEVPV AFVVRAADSD ITEESIKEFI SKQVVFYKRL 

       550        560 
HKVHFIHAIP KSASGKILRR ELRAKLAAC

« Hide

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References

« Hide 'large scale' references
[1]"4-coumarate:CoA ligase genes in rice: divergent structure and differential regulation."
Zhao Y., Kung S.D., Bottino P.J.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed: 16100779] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.
[3]"Curated genome annotation of Oryza sativa ssp. japonica and comparative genome analysis with Arabidopsis thaliana."
The rice annotation project (RAP)
Genome Res. 17:175-183(2007) [PubMed: 17210932] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: cv. Nipponbare.
[4]"Collection, mapping, and annotation of over 28,000 cDNA clones from japonica rice."
The rice full-length cDNA consortium
Science 301:376-379(2003) [PubMed: 12869764] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Nipponbare.
[5]"Genome-wide analysis of a land plant-specific acyl:coenzyme A synthetase (ACS) gene family in Arabidopsis, poplar, rice and Physcomitrella."
de Azevedo Souza C., Barbazuk B., Ralph S.G., Bohlmann J., Hamberger B., Douglas C.J.
New Phytol. 179:987-1003(2008) [PubMed: 18627494] [Abstract]
Cited for: GENE FAMILY.

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Cross-references

Sequence databases

L43362 mRNA. Translation: AAA69580.1.
AP004778 Genomic DNA. Translation: BAD07859.1.
AP005868 Genomic DNA. Translation: BAD08175.1.
AP008208 Genomic DNA. Translation: BAF09733.1.
AK105636 mRNA. No translation available.
PIRT03390.
RefSeqNP_001047819.1.
UniGeneOs.4377

3D structure databases

HSSPHSSP built from PDB template 1LCI based on UniProtKB P08659.
ModBaseSearch...

Genome annotation databases

GeneID4330407.
GenomeReviewsGene locus 4CL2 in contig AP008208_GR.
KEGGosa:4330407.

Organism-specific databases

GrameneQ42982.

Family and domain databases

InterProIPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamPF00501. AMP-binding. 1 hit.
[Graphical view]
PROSITEPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry name4CL2_ORYSJ
AccessionPrimary (citable) accession number: Q42982
Secondary accession number(s): Q0DYF5, Q6YUH6
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: September 27, 2005
Last modified: September 22, 2009
This is version 54 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents