Reviewed, UniProtKB/Swiss-Prot Q42972 (MDHG_ORYSJ)

Names and origin

Protein names

Recommended name:
    Malate dehydrogenase, glyoxysomal
    EC=1.1.1.37

Gene names

Ordered Locus Names:

Os12g0632700, LOC_Os12g43630

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH.
Subunit structure	Homodimer.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.
Sequence caution	The sequence ABA99939.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW malate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 36	36	Glyoxysome Ref.3
	Chain	37 – 356	320	Malate dehydrogenase, glyoxysomal		PRO_0000018640
Regions
	Nucleotide binding	51 – 57	7	NAD By similarity
	Nucleotide binding	160 – 162	3	NAD By similarity
Sites
	Active site	220	1	Proton acceptor By similarity
	Binding site	77	1	NAD By similarity
	Binding site	124	1	Substrate By similarity
	Binding site	130	1	Substrate By similarity
	Binding site	137	1	NAD By similarity
	Binding site	162	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	271	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42972-1 [UniParc]. Last modified July 25, 2006. Version 3. Checksum: D57BD93E93F381FB	FASTA	356	37,385
	10 20 30 40 50 60 MEDAAAAARR MERLASHLRP PASQMEESPL LRGSNCRAKG AAPGFKVAIL GASGGIGQPL 70 80 90 100 110 120 ALLMKMNPLV SVLHLYDVVN TPGVTADISH MNTGAVVRGF LGQPQLENAL TGMDLVIIPA 130 140 150 160 170 180 GVPRKPGMTR DDLFNINAGI VRTLCEGIAK CCPNAIVNVI SNPVNSTVPI AAEVFKKAGT 190 200 210 220 230 240 YDPKRLLGVT TLDVVRANTF VAEVLGLDPR DVNVPVIGGH AGVTILPLLS QVNPPCSFTS 250 260 270 280 290 300 EEISYLTTRI QNGGTEVVEA KAGAGSATLS MAYAASKFAD ACLRGLRGDA GIVECSFVAS 310 320 330 340 350 QVTELPFFAS KVRLGRCGIE EILSLGPLNE FERAGLEKAK KELAESIQKG VAFINK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a cDNA encoding Glyoxysomal malate dehydrogenase from rice (Oryza sativa L.)." Kaminaka H., Morita S., Masumura T., Tanaka K. Plant Gene Register PGR98-161 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed: 16758443] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 37-43, MASS SPECTROMETRY. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D85763 mRNA. Translation: BAA12870.1. DP000011 Genomic DNA. Translation: ABA99938.2. DP000011 Genomic DNA. Translation: ABA99939.2. Sequence problems.
PIR	T03272.
RefSeq	NP_001067346.1.
UniGene	Os.4155
3D structure databases
SMR	Q42972. Positions 44-354.
ModBase	Search...
Genome annotation databases
GeneID	4352871.
KEGG	osa:4352871.
Organism-specific databases
Gramene	Q42972.
Phylogenomic databases
PhylomeDB	Q42972.
Family and domain databases
InterPro	IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk/g-bac. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11540:SF1. MDH_euk_g_bac. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHG_ORYSJ

Accession

Primary (citable) accession number: Q42972
Secondary accession number(s): Q2QLQ8, Q2QLQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 25, 2006
Last modified:	February 9, 2010
This is version 75 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)