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Q42972

- MDHG_ORYSJ

UniProt

Q42972 - MDHG_ORYSJ

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Protein

Malate dehydrogenase, glyoxysomal

Gene

Os12g0632700

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771NADBy similarity
Binding sitei124 – 1241SubstratePROSITE-ProRule annotation
Binding sitei130 – 1301SubstratePROSITE-ProRule annotation
Binding sitei137 – 1371NADBy similarity
Binding sitei162 – 1621SubstratePROSITE-ProRule annotation
Binding sitei196 – 1961SubstratePROSITE-ProRule annotation
Active sitei220 – 2201Proton acceptorBy similarity
Binding sitei271 – 2711NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 577NADBy similarity
Nucleotide bindingi160 – 1623NADBy similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
Gene namesi
Ordered Locus Names:Os12g0632700, LOC_Os12g43630
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 12

Organism-specific databases

GrameneiQ42972.

Subcellular locationi

GO - Cellular componenti

  1. glyoxysome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636Glyoxysome1 PublicationAdd
BLAST
Chaini37 – 356320Malate dehydrogenase, glyoxysomalPRO_0000018640Add
BLAST

Proteomic databases

PRIDEiQ42972.

Expressioni

Gene expression databases

ExpressionAtlasiQ42972. baseline.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi39947.LOC_Os12g43630.1.

Structurei

3D structure databases

ProteinModelPortaliQ42972.
SMRiQ42972. Positions 44-354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
InParanoidiQ42972.
OMAiLGTHGVE.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42972-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEDAAAAARR MERLASHLRP PASQMEESPL LRGSNCRAKG AAPGFKVAIL
60 70 80 90 100
GASGGIGQPL ALLMKMNPLV SVLHLYDVVN TPGVTADISH MNTGAVVRGF
110 120 130 140 150
LGQPQLENAL TGMDLVIIPA GVPRKPGMTR DDLFNINAGI VRTLCEGIAK
160 170 180 190 200
CCPNAIVNVI SNPVNSTVPI AAEVFKKAGT YDPKRLLGVT TLDVVRANTF
210 220 230 240 250
VAEVLGLDPR DVNVPVIGGH AGVTILPLLS QVNPPCSFTS EEISYLTTRI
260 270 280 290 300
QNGGTEVVEA KAGAGSATLS MAYAASKFAD ACLRGLRGDA GIVECSFVAS
310 320 330 340 350
QVTELPFFAS KVRLGRCGIE EILSLGPLNE FERAGLEKAK KELAESIQKG

VAFINK
Length:356
Mass (Da):37,385
Last modified:July 25, 2006 - v3
Checksum:iD57BD93E93F381FB
GO

Sequence cautioni

The sequence ABA99939.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D85763 mRNA. Translation: BAA12870.1.
DP000011 Genomic DNA. Translation: ABA99938.2.
DP000011 Genomic DNA. Translation: ABA99939.2. Sequence problems.
AP008218 Genomic DNA. No translation available.
PIRiT03272.
UniGeneiOs.4155.

Genome annotation databases

EnsemblPlantsiOS12T0632700-01; OS12T0632700-01; OS12G0632700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D85763 mRNA. Translation: BAA12870.1 .
DP000011 Genomic DNA. Translation: ABA99938.2 .
DP000011 Genomic DNA. Translation: ABA99939.2 . Sequence problems.
AP008218 Genomic DNA. No translation available.
PIRi T03272.
UniGenei Os.4155.

3D structure databases

ProteinModelPortali Q42972.
SMRi Q42972. Positions 44-354.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os12g43630.1.

Proteomic databases

PRIDEi Q42972.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS12T0632700-01 ; OS12T0632700-01 ; OS12G0632700 .

Organism-specific databases

Gramenei Q42972.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000213792.
InParanoidi Q42972.
OMAi LGTHGVE.

Gene expression databases

ExpressionAtlasi Q42972. baseline.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a cDNA encoding Glyoxysomal malate dehydrogenase from rice (Oryza sativa L.)."
    Kaminaka H., Morita S., Masumura T., Tanaka K.
    Plant Gene Register PGR98-161
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
  2. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Proteomic analysis of rice leaf, stem and root tissues during growth course."
    Nozu Y., Tsugita A., Kamijo K.
    Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 37-43, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiMDHG_ORYSJ
AccessioniPrimary (citable) accession number: Q42972
Secondary accession number(s): Q2QLQ8, Q2QLQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 25, 2006
Last modified: October 29, 2014
This is version 108 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3