Reviewed,
UniProtKB/Swiss-Prot Q42972 (MDHG_ORYSJ)
Last modified
February 9, 2010.
Version 75.
History...
Clusters with 100%,
90%,
50% identity |
Documents (2) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Malate dehydrogenase, glyoxysomal EC=1.1.1.37 | ||
| Gene names |
| ||
| Organism | Oryza sativa subsp. japonica (Rice) | ||
| Taxonomic identifier | 39947 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza |
Protein attributes
| Sequence length | 356 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | (S)-malate + NAD+ = oxaloacetate + NADH. |
| Subunit structure | Homodimer. |
| Subcellular location | |
| Sequence similarities | Belongs to the LDH/MDH superfamily. MDH type 1 family. |
| Sequence caution | The sequence ABA99939.2 differs from that shown. Reason: Erroneous gene model prediction. |
Ontologies
| Keywords | |
|---|---|
| Biological process | Glyoxylate bypass Tricarboxylic acid cycle |
| Cellular component | Glyoxysome Peroxisome |
| Domain | Transit peptide |
| Ligand | NAD |
| Molecular function | Oxidoreductase |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | glycolysis Inferred from electronic annotation. Source: InterPro glyoxylate cycleInferred from electronic annotation. Source: UniProtKB-KW malate metabolic processInferred from electronic annotation. Source: InterPro oxidation reductionInferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycleInferred from electronic annotation. Source: UniProtKB-KW |
| Cellular component | glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell |
| Molecular function | L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC bindingInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Transit peptide | 1 – 36 | 36 | Glyoxysome Ref.3 | ||||||
| Chain | 37 – 356 | 320 | Malate dehydrogenase, glyoxysomal | PRO_0000018640 | |||||
Regions | |||||||||
| Nucleotide binding | 51 – 57 | 7 | NAD By similarity | ||||||
| Nucleotide binding | 160 – 162 | 3 | NAD By similarity | ||||||
Sites | |||||||||
| Active site | 220 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 77 | 1 | NAD By similarity | ||||||
| Binding site | 124 | 1 | Substrate By similarity | ||||||
| Binding site | 130 | 1 | Substrate By similarity | ||||||
| Binding site | 137 | 1 | NAD By similarity | ||||||
| Binding site | 162 | 1 | Substrate By similarity | ||||||
| Binding site | 196 | 1 | Substrate By similarity | ||||||
| Binding site | 271 | 1 | NAD By similarity | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning and characterization of a cDNA encoding Glyoxysomal malate dehydrogenase from rice (Oryza sativa L.)." Kaminaka H., Morita S., Masumura T., Tanaka K. Plant Gene Register PGR98-161 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare. |
| [2] | "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed: 16188032] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare. |
| [3] | "Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed: 16758443] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 37-43, MASS SPECTROMETRY. Strain: cv. Nipponbare. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | D85763 mRNA. Translation: BAA12870.1. DP000011 Genomic DNA. Translation: ABA99938.2. DP000011 Genomic DNA. Translation: ABA99939.2. Sequence problems. |
| PIR | T03272. |
| RefSeq | NP_001067346.1. |
| UniGene | Os.4155 |
3D structure databases | |
| SMR | Q42972. Positions 44-354. |
| ModBase | Search... |
Genome annotation databases | |
| GeneID | 4352871. |
| KEGG | osa:4352871. |
Organism-specific databases | |
| Gramene | Q42972. |
Phylogenomic databases | |
| PhylomeDB | Q42972. |
Family and domain databases | |
| InterPro | IPR001557. L-lactate/malate_DH. IPR001236. Lactate/malate_DH. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_NAD-dep_euk/g-bac. IPR016040. NAD(P)-bd_dom. [Graphical view] |
| Gene3D | G3DSA:3.90.110.10. lact_mal_DH. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. |
| PANTHER | PTHR11540:SF1. MDH_euk_g_bac. 1 hit. |
| Pfam | PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view] |
| PIRSF | PIRSF000102. Lac_mal_DH. 1 hit. |
| TIGRFAMs | TIGR01772. MDH_euk_gproteo. 1 hit. |
| PROSITE | PS00068. MDH. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | MDHG_ORYSJ | ||||||||
| Accession | Primary (citable) accession number: Q42972 Secondary accession number(s): Q2QLQ8, Q2QLQ9 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | PPAP (Plant Proteome Annotation Project) | ||||||||
Relevant documents
| Oryza sativa (rice) Index of Oryza sativa entries and their corresponding gene designations |
| SIMILARITY comments Index of protein domains and families |

Clusters with


