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Q42972

- MDHG_ORYSJ

UniProt

Q42972 - MDHG_ORYSJ

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Protein
Malate dehydrogenase, glyoxysomal
Gene
Os12g0632700, LOC_Os12g43630
Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei77 – 771NAD By similarity
Binding sitei124 – 1241Substrate By similarity
Binding sitei130 – 1301Substrate By similarity
Binding sitei137 – 1371NAD By similarity
Binding sitei162 – 1621Substrate By similarity
Binding sitei196 – 1961Substrate By similarity
Active sitei220 – 2201Proton acceptor By similarity
Binding sitei271 – 2711NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi51 – 577NAD By similarity
Nucleotide bindingi160 – 1623NAD By similarity

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-KW
  2. malate metabolic process Source: InterPro
  3. regulation of fatty acid beta-oxidation Source: EnsemblPlants/Gramene
  4. regulation of photorespiration Source: EnsemblPlants/Gramene
  5. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
Gene namesi
Ordered Locus Names:Os12g0632700, LOC_Os12g43630
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
ProteomesiUP000000763: Chromosome 12

Organism-specific databases

GrameneiQ42972.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: EnsemblPlants/Gramene
  2. glyoxysome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3636Glyoxysome1 Publication
Add
BLAST
Chaini37 – 356320Malate dehydrogenase, glyoxysomal
PRO_0000018640Add
BLAST

Proteomic databases

PRIDEiQ42972.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi39947.LOC_Os12g43630.1.

Structurei

3D structure databases

ProteinModelPortaliQ42972.
SMRiQ42972. Positions 44-354.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213792.
OMAiLGTHGVE.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42972-1 [UniParc]FASTAAdd to Basket

« Hide

MEDAAAAARR MERLASHLRP PASQMEESPL LRGSNCRAKG AAPGFKVAIL    50
GASGGIGQPL ALLMKMNPLV SVLHLYDVVN TPGVTADISH MNTGAVVRGF 100
LGQPQLENAL TGMDLVIIPA GVPRKPGMTR DDLFNINAGI VRTLCEGIAK 150
CCPNAIVNVI SNPVNSTVPI AAEVFKKAGT YDPKRLLGVT TLDVVRANTF 200
VAEVLGLDPR DVNVPVIGGH AGVTILPLLS QVNPPCSFTS EEISYLTTRI 250
QNGGTEVVEA KAGAGSATLS MAYAASKFAD ACLRGLRGDA GIVECSFVAS 300
QVTELPFFAS KVRLGRCGIE EILSLGPLNE FERAGLEKAK KELAESIQKG 350
VAFINK 356
Length:356
Mass (Da):37,385
Last modified:July 25, 2006 - v3
Checksum:iD57BD93E93F381FB
GO

Sequence cautioni

The sequence ABA99939.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D85763 mRNA. Translation: BAA12870.1.
DP000011 Genomic DNA. Translation: ABA99938.2.
DP000011 Genomic DNA. Translation: ABA99939.2. Sequence problems.
AP008218 Genomic DNA. No translation available.
PIRiT03272.
UniGeneiOs.4155.

Genome annotation databases

EnsemblPlantsiOS12T0632700-01; OS12T0632700-01; OS12G0632700.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D85763 mRNA. Translation: BAA12870.1 .
DP000011 Genomic DNA. Translation: ABA99938.2 .
DP000011 Genomic DNA. Translation: ABA99939.2 . Sequence problems.
AP008218 Genomic DNA. No translation available.
PIRi T03272.
UniGenei Os.4155.

3D structure databases

ProteinModelPortali Q42972.
SMRi Q42972. Positions 44-354.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 39947.LOC_Os12g43630.1.

Proteomic databases

PRIDEi Q42972.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi OS12T0632700-01 ; OS12T0632700-01 ; OS12G0632700 .

Organism-specific databases

Gramenei Q42972.

Phylogenomic databases

eggNOGi COG0039.
HOGENOMi HOG000213792.
OMAi LGTHGVE.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11540. PTHR11540. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of a cDNA encoding Glyoxysomal malate dehydrogenase from rice (Oryza sativa L.)."
    Kaminaka H., Morita S., Masumura T., Tanaka K.
    Plant Gene Register PGR98-161
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Nipponbare.
  2. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
    The rice chromosomes 11 and 12 sequencing consortia
    BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Nipponbare.
  4. "The rice annotation project database (RAP-DB): 2008 update."
    The rice annotation project (RAP)
    Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENOME REANNOTATION.
    Strain: cv. Nipponbare.
  5. "Proteomic analysis of rice leaf, stem and root tissues during growth course."
    Nozu Y., Tsugita A., Kamijo K.
    Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 37-43, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: cv. Nipponbare.

Entry informationi

Entry nameiMDHG_ORYSJ
AccessioniPrimary (citable) accession number: Q42972
Secondary accession number(s): Q2QLQ8, Q2QLQ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 25, 2006
Last modified: September 3, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi