Malate dehydrogenase, glyoxysomal precursor - Oryza sativa subsp. japonica (Rice)

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Q42972 (MDHG_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Malate dehydrogenase, glyoxysomal
EC=1.1.1.37

Gene names

Ordered Locus Names:

Os12g0632700, LOC_Os12g43630

Organism

Oryza sativa subsp. japonica (Rice) [Reference proteome]

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza ›

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(S)-malate + NAD⁺ = oxaloacetate + NADH.
Subunit structure	Homodimer.
Subcellular location	Glyoxysome.
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 1 family.
Sequence caution	The sequence ABA99939.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Glyoxysome Peroxisome
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW malate metabolic process Inferred from electronic annotation. Source: InterPro regulation of fatty acid beta-oxidation Inferred from electronic annotation. Source: EnsemblPlants/Gramene regulation of photorespiration Inferred from electronic annotation. Source: EnsemblPlants/Gramene tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	apoplast Inferred from electronic annotation. Source: EnsemblPlants/Gramene chloroplast envelope Inferred from electronic annotation. Source: EnsemblPlants/Gramene glyoxysome Inferred from electronic annotation. Source: UniProtKB-SubCell vacuole Inferred from electronic annotation. Source: EnsemblPlants/Gramene
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 36

Glyoxysome Ref.3

Chain

37 – 356

320

Malate dehydrogenase, glyoxysomal

PRO_0000018640

Regions

Nucleotide binding

51 – 57

NAD By similarity

Nucleotide binding

160 – 162

NAD By similarity

Sites

Active site

220

Proton acceptor By similarity

Binding site

NAD By similarity

Binding site

124

Substrate By similarity

Binding site

130

Substrate By similarity

Binding site

137

NAD By similarity

Binding site

162

Substrate By similarity

Binding site

196

Substrate By similarity

Binding site

271

NAD By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

Q42972 [UniParc].

Last modified July 25, 2006. Version 3.
Checksum: D57BD93E93F381FB
Show »

FASTA

356

37,385

        10         20         30         40         50         60 
MEDAAAAARR MERLASHLRP PASQMEESPL LRGSNCRAKG AAPGFKVAIL GASGGIGQPL 

        70         80         90        100        110        120 
ALLMKMNPLV SVLHLYDVVN TPGVTADISH MNTGAVVRGF LGQPQLENAL TGMDLVIIPA 

       130        140        150        160        170        180 
GVPRKPGMTR DDLFNINAGI VRTLCEGIAK CCPNAIVNVI SNPVNSTVPI AAEVFKKAGT 

       190        200        210        220        230        240 
YDPKRLLGVT TLDVVRANTF VAEVLGLDPR DVNVPVIGGH AGVTILPLLS QVNPPCSFTS 

       250        260        270        280        290        300 
EEISYLTTRI QNGGTEVVEA KAGAGSATLS MAYAASKFAD ACLRGLRGDA GIVECSFVAS 

       310        320        330        340        350 
QVTELPFFAS KVRLGRCGIE EILSLGPLNE FERAGLEKAK KELAESIQKG VAFINK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of a cDNA encoding Glyoxysomal malate dehydrogenase from rice (Oryza sativa L.)." Kaminaka H., Morita S., Masumura T., Tanaka K. Plant Gene Register PGR98-161 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Nipponbare.
[2]	"The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications." The rice chromosomes 11 and 12 sequencing consortia BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Proteomic analysis of rice leaf, stem and root tissues during growth course." Nozu Y., Tsugita A., Kamijo K. Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 37-43, MASS SPECTROMETRY. Strain: cv. Nipponbare.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D85763 mRNA. Translation: BAA12870.1. DP000011 Genomic DNA. Translation: ABA99938.2. DP000011 Genomic DNA. Translation: ABA99939.2. Sequence problems.
PIR	T03272.
UniGene	Os.4155.
3D structure databases
ProteinModelPortal	Q42972.
SMR	Q42972. Positions 44-354.
ModBase	Search...
Protein-protein interaction databases
STRING	39947.LOC_Os12g43630.1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os12g43630.1; LOC_Os12g43630.1; LOC_Os12g43630.
Organism-specific databases
Gramene	Q42972.
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213792.
OMA	NPLITEL.
Gene expression databases
ArrayExpress	Q42972.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR001252. Malate_DH_AS. IPR010097. Malate_DH_type1. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit. PTHR11540:SF1. PTHR11540:SF1. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
TIGRFAMs	TIGR01772. MDH_euk_gproteo. 1 hit.
PROSITE	PS00068. MDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MDHG_ORYSJ

Accession

Primary (citable) accession number: Q42972
Secondary accession number(s): Q2QLQ8, Q2QLQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 25, 2006
Last modified:	April 3, 2013
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents