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Q42972

- MDHG_ORYSJ

UniProt

Q42972 - MDHG_ORYSJ

Protein

Malate dehydrogenase, glyoxysomal

Gene

Os12g0632700

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 3 (25 Jul 2006)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771NADBy similarity
    Binding sitei124 – 1241SubstratePROSITE-ProRule annotation
    Binding sitei130 – 1301SubstratePROSITE-ProRule annotation
    Binding sitei137 – 1371NADBy similarity
    Binding sitei162 – 1621SubstratePROSITE-ProRule annotation
    Binding sitei196 – 1961SubstratePROSITE-ProRule annotation
    Active sitei220 – 2201Proton acceptorBy similarity
    Binding sitei271 – 2711NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi51 – 577NADBy similarity
    Nucleotide bindingi160 – 1623NADBy similarity

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-KW
    2. malate metabolic process Source: InterPro
    3. regulation of fatty acid beta-oxidation Source: EnsemblPlants/Gramene
    4. regulation of photorespiration Source: EnsemblPlants/Gramene
    5. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenase, glyoxysomal (EC:1.1.1.37)
    Gene namesi
    Ordered Locus Names:Os12g0632700, LOC_Os12g43630
    OrganismiOryza sativa subsp. japonica (Rice)
    Taxonomic identifieri39947 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza
    ProteomesiUP000000763: Chromosome 12

    Organism-specific databases

    GrameneiQ42972.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: EnsemblPlants/Gramene
    2. glyoxysome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Glyoxysome, Peroxisome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3636Glyoxysome1 PublicationAdd
    BLAST
    Chaini37 – 356320Malate dehydrogenase, glyoxysomalPRO_0000018640Add
    BLAST

    Proteomic databases

    PRIDEiQ42972.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi39947.LOC_Os12g43630.1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ42972.
    SMRiQ42972. Positions 44-354.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 1 family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0039.
    HOGENOMiHOG000213792.
    OMAiLGTHGVE.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q42972-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDAAAAARR MERLASHLRP PASQMEESPL LRGSNCRAKG AAPGFKVAIL    50
    GASGGIGQPL ALLMKMNPLV SVLHLYDVVN TPGVTADISH MNTGAVVRGF 100
    LGQPQLENAL TGMDLVIIPA GVPRKPGMTR DDLFNINAGI VRTLCEGIAK 150
    CCPNAIVNVI SNPVNSTVPI AAEVFKKAGT YDPKRLLGVT TLDVVRANTF 200
    VAEVLGLDPR DVNVPVIGGH AGVTILPLLS QVNPPCSFTS EEISYLTTRI 250
    QNGGTEVVEA KAGAGSATLS MAYAASKFAD ACLRGLRGDA GIVECSFVAS 300
    QVTELPFFAS KVRLGRCGIE EILSLGPLNE FERAGLEKAK KELAESIQKG 350
    VAFINK 356
    Length:356
    Mass (Da):37,385
    Last modified:July 25, 2006 - v3
    Checksum:iD57BD93E93F381FB
    GO

    Sequence cautioni

    The sequence ABA99939.2 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D85763 mRNA. Translation: BAA12870.1.
    DP000011 Genomic DNA. Translation: ABA99938.2.
    DP000011 Genomic DNA. Translation: ABA99939.2. Sequence problems.
    AP008218 Genomic DNA. No translation available.
    PIRiT03272.
    UniGeneiOs.4155.

    Genome annotation databases

    EnsemblPlantsiOS12T0632700-01; OS12T0632700-01; OS12G0632700.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D85763 mRNA. Translation: BAA12870.1 .
    DP000011 Genomic DNA. Translation: ABA99938.2 .
    DP000011 Genomic DNA. Translation: ABA99939.2 . Sequence problems.
    AP008218 Genomic DNA. No translation available.
    PIRi T03272.
    UniGenei Os.4155.

    3D structure databases

    ProteinModelPortali Q42972.
    SMRi Q42972. Positions 44-354.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 39947.LOC_Os12g43630.1.

    Proteomic databases

    PRIDEi Q42972.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi OS12T0632700-01 ; OS12T0632700-01 ; OS12G0632700 .

    Organism-specific databases

    Gramenei Q42972.

    Phylogenomic databases

    eggNOGi COG0039.
    HOGENOMi HOG000213792.
    OMAi LGTHGVE.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010097. Malate_DH_type1.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01772. MDH_euk_gproteo. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of a cDNA encoding Glyoxysomal malate dehydrogenase from rice (Oryza sativa L.)."
      Kaminaka H., Morita S., Masumura T., Tanaka K.
      Plant Gene Register PGR98-161
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Nipponbare.
    2. "The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications."
      The rice chromosomes 11 and 12 sequencing consortia
      BMC Biol. 3:20-20(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Nipponbare.
    4. "The rice annotation project database (RAP-DB): 2008 update."
      The rice annotation project (RAP)
      Nucleic Acids Res. 36:D1028-D1033(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENOME REANNOTATION.
      Strain: cv. Nipponbare.
    5. "Proteomic analysis of rice leaf, stem and root tissues during growth course."
      Nozu Y., Tsugita A., Kamijo K.
      Proteomics 6:3665-3670(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE [LARGE SCALE ANALYSIS] OF 37-43, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: cv. Nipponbare.

    Entry informationi

    Entry nameiMDHG_ORYSJ
    AccessioniPrimary (citable) accession number: Q42972
    Secondary accession number(s): Q2QLQ8, Q2QLQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 25, 2006
    Last modified: October 1, 2014
    This is version 107 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Oryza sativa (rice)
      Index of Oryza sativa entries and their corresponding gene designations
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3