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UniProtKB - Q42971 (ENO_ORYSJ)

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Protein

Enolase

Gene

ENO1

Organism
Oryza sativa subsp. japonica (Rice)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+Note: Mg2+ is required for catalysis and for stabilizing the dimer.

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolic (GAPC3), Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic (GAPC1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Enolase (ENO1)
  5. Pyruvate kinase (OsJ_23327), Pyruvate kinase (Os04g0677500), Pyruvate kinase (Os10g0571200), Pyruvate kinase (Os07g0181000), Pyruvate kinase (Os10g0571200), Pyruvate kinase (OsJ_12060), Pyruvate kinase 2, cytosolic (Os12g0145700), Pyruvate kinase (LOC_Os12g05110), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os03g0325000), Pyruvate kinase (Os01g0660300), Pyruvate kinase (OJ1014_E09.29-2), Pyruvate kinase, Pyruvate kinase (Os11g0216000), Pyruvate kinase (LOC_Os03g46910), Pyruvate kinase 1, cytosolic (Os11g0148500), Pyruvate kinase (OsJ_10677), Pyruvate kinase (P0445H04.13), Pyruvate kinase (OsJ_32540), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os07g0181000), Pyruvate kinase (OJ1014_E09.29-1), Pyruvate kinase, Pyruvate kinase (Os11g0216000), Pyruvate kinase (OsJ_02894), Pyruvate kinase (LOC_Os03g20880), Pyruvate kinase (Os07g0181000)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei164SubstrateBy similarity1
Binding sitei173SubstrateBy similarity1
Active sitei216Proton donorBy similarity1
Metal bindingi251MagnesiumBy similarity1
Metal bindingi302MagnesiumBy similarity1
Binding sitei302SubstrateBy similarity1
Metal bindingi329MagnesiumBy similarity1
Binding sitei329SubstrateBy similarity1
Active sitei354Proton acceptorBy similarity1
Binding sitei405SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

GO - Biological processi

Complete GO annotation...

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
OSE1
Gene namesi
Name:ENO1
Synonyms:AD709
Ordered Locus Names:Os10g0167300, LOC_Os10g08550
ORF Names:OSJNAb0015J03.9
OrganismiOryza sativa subsp. japonica (Rice)
Taxonomic identifieri39947 [NCBI]
Taxonomic lineageiEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza ›
Proteomesi
  • UP000059680 Componenti: Chromosome 10

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340761 – 446EnolaseAdd BLAST446

Proteomic databases

PaxDbiQ42971.
PRIDEiQ42971.

Expressioni

Developmental stagei

Expressed during early embryogenesis.

Gene expression databases

GenevisibleiQ42971. OS.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi39947.LOC_Os10g08550.1.

Structurei

3D structure databases

ProteinModelPortaliQ42971.
SMRiQ42971.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni381 – 384Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGiKOG2670. Eukaryota.
COG0148. LUCA.
InParanoidiQ42971.
KOiK01689.
OrthoDBiEOG093608UK.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiView protein in InterPro
IPR000941. Enolase.
IPR034390. Enolase-like_superfamily.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiView protein in Pfam
PF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SFLDiSFLDG00178. enolase. 1 hit.
SFLDS00001. Enolase. 1 hit.
SMARTiView protein in SMART
SM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiView protein in PROSITE
PS00164. ENOLASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q42971-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAATIVSVKA RQIFDSRGNP TVEVDVCCSD GTFARAAVPS GASTGVYEAL 
        60         70         80         90        100
ELRDGGSDYL GKGVSKAVDN VNSVIAPALI GKDPTSQAEL DNFMVQQLDG 
       110        120        130        140        150
TKNEWGWCKQ KLGANAILAV SLAICKAGAI IKKIPLYQHI ANLAGNKQLV 
       160        170        180        190        200
LPVPAFNVIN GGSHAGNKLA MQEFMILPTG AASFKEAMKM GVEVYHNLKS 
       210        220        230        240        250
VIKKKYGQDA TNVGDEGGFA PNIQENKEGL ELLKTAIEKA GYTGKVVIGM 
       260        270        280        290        300
DVAASEFYND KDKTYDLNFK EENNDGSQKI SGDSLKNVYK SFVSEYPIVS 
       310        320        330        340        350
IEDPFDQDDW EHYAKMTAEI GEQVQIVGDD LLVTNPTRVA KAIQEKSCNA 
       360        370        380        390        400
LLLKVNQIGS VTESIEAVKM SKRAGWGVMT SHRSGETEDT FIADLAVGLA 
       410        420        430        440 
TGQIKTGAPC RSERLAKYNQ LLRIEEELGA AAVYAGAKFR APVEPY
Length:446
Mass (Da):47,973
Last modified:March 7, 2006 - v2
Checksum:iBFCD813193800F1B
GO

Sequence cautioni

The sequence AAN04181 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABB46861 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABB46862 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABG65935 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti394D → E in AAC49173 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09450 mRNA. Translation: AAC49173.1.
AY335488 mRNA. Translation: AAP94211.1.
AC131375 Genomic DNA. Translation: AAN04181.1. Sequence problems.
DP000086 Genomic DNA. Translation: ABB46861.2. Sequence problems.
DP000086 Genomic DNA. Translation: ABB46862.2. Sequence problems.
DP000086 Genomic DNA. Translation: ABG65935.1. Sequence problems.
AP008216 Genomic DNA. Translation: BAF26137.1.
AP014966 Genomic DNA. No translation available.
D17767 mRNA. Translation: BAA04612.1.
PIRiT03267.
RefSeqiXP_015614256.1. XM_015758770.1.
UniGeneiOs.57462.

Genome annotation databases

GeneIDi4348176.
KEGGiosa:4348176.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiENO_ORYSJ
AccessioniPrimary (citable) accession number: Q42971
Secondary accession number(s): Q0IYS8
, Q10A26, Q33AR3, Q33AR4, Q42987, Q7XBE4, Q8LM12
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: March 7, 2006
Last modified: April 12, 2017
This is version 121 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Oryza sativa (rice)
    Index of Oryza sativa entries and their corresponding gene designations
  3. SIMILARITY comments
    Index of protein domains and families