UniProtKB - Q42971 (ENO_ORYSJ)
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Protein
Enolase
Gene
ENO1
Organism
Oryza sativa subsp. japonica (Rice)
Status
Functioni
Catalytic activityi
2-phospho-D-glycerate = phosphoenolpyruvate + H2O.
Cofactori
Mg2+Note: Mg2+ is required for catalysis and for stabilizing the dimer.
: glycolysis Pathwayi
This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolic (GAPC3), Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic (GAPC1)
- no protein annotated in this organism
- no protein annotated in this organism
- Enolase (ENO1)
- Pyruvate kinase (OsJ_23327), Pyruvate kinase (Os04g0677500), Pyruvate kinase (LOC_Os10g42100), Pyruvate kinase (Os07g0181000), Pyruvate kinase (Os10g0571200), Pyruvate kinase (OsJ_12060), Pyruvate kinase 2, cytosolic (Os12g0145700), Pyruvate kinase (LOC_Os12g05110), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os03g0325000), Pyruvate kinase (Os01g0660300), Pyruvate kinase (OJ1014_E09.29-2), Pyruvate kinase, Pyruvate kinase (Os11g0216000), Pyruvate kinase (Os03g0672300), Pyruvate kinase 1, cytosolic (Os11g0148500), Pyruvate kinase (OsJ_10677), Pyruvate kinase (P0445H04.13), Pyruvate kinase (OsJ_32540), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os07g0181000), Pyruvate kinase (OJ1014_E09.29-1), Pyruvate kinase, Pyruvate kinase (Os11g0216000), Pyruvate kinase (OsJ_02894), Pyruvate kinase (Os07g0181000), Pyruvate kinase (LOC_Os03g20880)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 164 | SubstrateBy similarity | 1 | |
Binding sitei | 173 | SubstrateBy similarity | 1 | |
Active sitei | 216 | Proton donorBy similarity | 1 | |
Metal bindingi | 251 | MagnesiumBy similarity | 1 | |
Metal bindingi | 302 | MagnesiumBy similarity | 1 | |
Binding sitei | 302 | SubstrateBy similarity | 1 | |
Metal bindingi | 329 | MagnesiumBy similarity | 1 | |
Binding sitei | 329 | SubstrateBy similarity | 1 | |
Active sitei | 354 | Proton acceptorBy similarity | 1 | |
Binding sitei | 405 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- magnesium ion binding Source: InterPro
- phosphopyruvate hydratase activity Source: UniProtKB-EC
GO - Biological processi
- glycolytic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Lyase |
Biological process | Glycolysis |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
UniPathwayi | UPA00109; UER00187. |
Names & Taxonomyi
Protein namesi | Recommended name: Enolase (EC:4.2.1.11)Alternative name(s): 2-phospho-D-glycerate hydro-lyase 2-phosphoglycerate dehydratase OSE1 |
Gene namesi | Name:ENO1 Synonyms:AD709 Ordered Locus Names:Os10g0167300, LOC_Os10g08550 ORF Names:OSJNAb0015J03.9 |
Organismi | Oryza sativa subsp. japonica (Rice) |
Taxonomic identifieri | 39947 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000134076 | 1 – 446 | EnolaseAdd BLAST | 446 |
Proteomic databases
PaxDbi | Q42971. |
PRIDEi | Q42971. |
Expressioni
Developmental stagei
Expressed during early embryogenesis.
Gene expression databases
ExpressionAtlasi | Q42971. baseline and differential. |
Genevisiblei | Q42971. OS. |
Interactioni
Subunit structurei
Homodimer.By similarity
Protein-protein interaction databases
STRINGi | 39947.LOC_Os10g08550.1. |
Structurei
3D structure databases
ProteinModelPortali | Q42971. |
SMRi | Q42971. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 381 – 384 | Substrate bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the enolase family.Curated
Phylogenomic databases
eggNOGi | KOG2670. Eukaryota. COG0148. LUCA. |
InParanoidi | Q42971. |
KOi | K01689. |
OrthoDBi | EOG093608UK. |
Family and domain databases
CDDi | cd03313. enolase. 1 hit. |
Gene3Di | 3.20.20.120. 1 hit. 3.30.390.10. 1 hit. |
HAMAPi | MF_00318. Enolase. 1 hit. |
InterProi | View protein in InterPro IPR000941. Enolase. IPR036849. Enolase-like_C_sf. IPR029017. Enolase-like_N. IPR034390. Enolase-like_superfamily. IPR020810. Enolase_C. IPR020809. Enolase_CS. IPR020811. Enolase_N. |
PANTHERi | PTHR11902. PTHR11902. 1 hit. |
Pfami | View protein in Pfam PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit. |
PIRSFi | PIRSF001400. Enolase. 1 hit. |
PRINTSi | PR00148. ENOLASE. |
SFLDi | SFLDG00178. enolase. 1 hit. SFLDS00001. Enolase. 1 hit. |
SMARTi | View protein in SMART SM01192. Enolase_C. 1 hit. SM01193. Enolase_N. 1 hit. |
SUPFAMi | SSF51604. SSF51604. 1 hit. |
TIGRFAMsi | TIGR01060. eno. 1 hit. |
PROSITEi | View protein in PROSITE PS00164. ENOLASE. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q42971-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAATIVSVKA RQIFDSRGNP TVEVDVCCSD GTFARAAVPS GASTGVYEAL
60 70 80 90 100
ELRDGGSDYL GKGVSKAVDN VNSVIAPALI GKDPTSQAEL DNFMVQQLDG
110 120 130 140 150
TKNEWGWCKQ KLGANAILAV SLAICKAGAI IKKIPLYQHI ANLAGNKQLV
160 170 180 190 200
LPVPAFNVIN GGSHAGNKLA MQEFMILPTG AASFKEAMKM GVEVYHNLKS
210 220 230 240 250
VIKKKYGQDA TNVGDEGGFA PNIQENKEGL ELLKTAIEKA GYTGKVVIGM
260 270 280 290 300
DVAASEFYND KDKTYDLNFK EENNDGSQKI SGDSLKNVYK SFVSEYPIVS
310 320 330 340 350
IEDPFDQDDW EHYAKMTAEI GEQVQIVGDD LLVTNPTRVA KAIQEKSCNA
360 370 380 390 400
LLLKVNQIGS VTESIEAVKM SKRAGWGVMT SHRSGETEDT FIADLAVGLA
410 420 430 440
TGQIKTGAPC RSERLAKYNQ LLRIEEELGA AAVYAGAKFR APVEPY
Sequence cautioni
The sequence AAN04181 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABB46861 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABB46862 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence ABG65935 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 394 | D → E in AAC49173 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U09450 mRNA. Translation: AAC49173.1. AY335488 mRNA. Translation: AAP94211.1. AC131375 Genomic DNA. Translation: AAN04181.1. Sequence problems. DP000086 Genomic DNA. Translation: ABB46861.2. Sequence problems. DP000086 Genomic DNA. Translation: ABB46862.2. Sequence problems. DP000086 Genomic DNA. Translation: ABG65935.1. Sequence problems. AP008216 Genomic DNA. Translation: BAF26137.1. AP014966 Genomic DNA. No translation available. D17767 mRNA. Translation: BAA04612.1. |
PIRi | T03267. |
RefSeqi | XP_015614256.1. XM_015758770.1. |
UniGenei | Os.57462. |
Genome annotation databases
GeneIDi | 4348176. |
KEGGi | osa:4348176. |
Similar proteinsi
Entry informationi
Entry namei | ENO_ORYSJ | |
Accessioni | Q42971Primary (citable) accession number: Q42971 Secondary accession number(s): Q0IYS8 Q8LM12 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1998 |
Last sequence update: | March 7, 2006 | |
Last modified: | February 28, 2018 | |
This is version 126 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |