ENO1

Functionⁱ

Catalytic activityⁱ

2-phospho-D-glycerate = phosphoenolpyruvate + H₂O.

Cofactorⁱ

Mg²⁺Note: Mg²⁺ is required for catalysis and for stabilizing the dimer.

Pathwayⁱ: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

Glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase 3, cytosolic (GAPC3), Glyceraldehyde-3-phosphate dehydrogenase 1, cytosolic (GAPC1)
no protein annotated in this organism
no protein annotated in this organism
Enolase (ENO1)
Pyruvate kinase (OsJ_23327), Pyruvate kinase (Os04g0677500), Pyruvate kinase (Os10g0571200), Pyruvate kinase (Os07g0181000), Pyruvate kinase (Os10g0571200), Pyruvate kinase (OsJ_12060), Pyruvate kinase 2, cytosolic (Os12g0145700), Pyruvate kinase (LOC_Os12g05110), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os03g0325000), Pyruvate kinase (Os01g0660300), Pyruvate kinase (OJ1014_E09.29-2), Pyruvate kinase, Pyruvate kinase (Os11g0216000), Pyruvate kinase (LOC_Os03g46910), Pyruvate kinase 1, cytosolic (Os11g0148500), Pyruvate kinase (OsJ_10677), Pyruvate kinase (P0445H04.13), Pyruvate kinase (OsJ_32540), Pyruvate kinase (Os01g0276700), Pyruvate kinase (Os07g0181000), Pyruvate kinase, Pyruvate kinase (OJ1014_E09.29-1), Pyruvate kinase (Os11g0216000), Pyruvate kinase (OsJ_02894), Pyruvate kinase (Os07g0181000), Pyruvate kinase (LOC_Os03g20880)

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	164	SubstrateBy similarity	1
Binding siteⁱ	173	SubstrateBy similarity	1
Active siteⁱ	216	Proton donorBy similarity	1
Metal bindingⁱ	251	MagnesiumBy similarity	1
Metal bindingⁱ	302	MagnesiumBy similarity	1
Binding siteⁱ	302	SubstrateBy similarity	1
Metal bindingⁱ	329	MagnesiumBy similarity	1
Binding siteⁱ	329	SubstrateBy similarity	1
Active siteⁱ	354	Proton acceptorBy similarity	1
Binding siteⁱ	405	SubstrateBy similarity	1

GO - Molecular functionⁱ

magnesium ion binding Source: InterPro
phosphopyruvate hydratase activity Source: UniProtKB-EC

Complete GO annotation...

GO - Biological processⁱ

glycolytic process Source: UniProtKB-UniPathway

Complete GO annotation...

Keywordsⁱ

Molecular function	Lyase
Biological process	Glycolysis
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00187.

UniPathwayⁱ

UPA00109; UER00187.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Enolase (EC:4.2.1.11) Alternative name(s): 2-phospho-D-glycerate hydro-lyase 2-phosphoglycerate dehydratase OSE1
Gene namesⁱ	Name:ENO1 Synonyms:AD709 Ordered Locus Names:Os10g0167300, LOC_Os10g08550 ORF Names:OSJNAb0015J03.9
Organismⁱ	Oryza sativa subsp. japonica (Rice)
Taxonomic identifierⁱ	39947 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › Liliopsida › Petrosaviidae › commelinids › Poales › Poaceae › BOP clade › Oryzoideae › Oryzeae › Oryzinae › Oryza › Oryza sativa
Proteomesⁱ	UP000059680 Componentⁱ: Chromosome 10

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

phosphopyruvate hydratase complex Source: InterPro

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000134076	1 – 446	EnolaseAdd BLAST		446

Proteomic databases

PaxDbⁱ	Q42971.
PRIDEⁱ	Q42971.

Expressionⁱ

Developmental stageⁱ

Expressed during early embryogenesis.

Gene expression databases

Genevisibleⁱ	Q42971. OS.

Genevisibleⁱ

Q42971. OS.

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

Protein-protein interaction databases

STRINGⁱ	39947.LOC_Os10g08550.1.

STRINGⁱ

39947.LOC_Os10g08550.1.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q42971.
SMRⁱ	Q42971.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	381 – 384	Substrate bindingBy similarity		4

Sequence similaritiesⁱ

Belongs to the enolase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG2670. Eukaryota. COG0148. LUCA.
InParanoidⁱ	Q42971.
KEGG Orthology (KO) More...KOⁱ	K01689.
Database of Orthologous Groups More...OrthoDBⁱ	EOG093608UK.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313. enolase. 1 hit.
Gene3Dⁱ	3.20.20.120. 1 hit. 3.30.390.10. 1 hit.
HAMAPⁱ	MF_00318. Enolase. 1 hit.
InterProⁱ	View protein in InterPro IPR000941. Enolase. IPR034390. Enolase-like_superfamily. IPR020810. Enolase_C. IPR029065. Enolase_C-like. IPR020809. Enolase_CS. IPR020811. Enolase_N. IPR029017. Enolase_N-like.
PANTHERⁱ	PTHR11902. PTHR11902. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit.
PIRSFⁱ	PIRSF001400. Enolase. 1 hit.
PRINTSⁱ	PR00148. ENOLASE.
SFLDⁱ	SFLDG00178. enolase. 1 hit. SFLDS00001. Enolase. 1 hit.
SMARTⁱ	View protein in SMART SM01192. Enolase_C. 1 hit. SM01193. Enolase_N. 1 hit.
SUPFAMⁱ	SSF51604. SSF51604. 1 hit. SSF54826. SSF54826. 1 hit.
TIGRFAMsⁱ	TIGR01060. eno. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00164. ENOLASE. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q42971-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAATIVSVKA RQIFDSRGNP TVEVDVCCSD GTFARAAVPS GASTGVYEAL 
        60         70         80         90        100
ELRDGGSDYL GKGVSKAVDN VNSVIAPALI GKDPTSQAEL DNFMVQQLDG 
       110        120        130        140        150
TKNEWGWCKQ KLGANAILAV SLAICKAGAI IKKIPLYQHI ANLAGNKQLV 
       160        170        180        190        200
LPVPAFNVIN GGSHAGNKLA MQEFMILPTG AASFKEAMKM GVEVYHNLKS 
       210        220        230        240        250
VIKKKYGQDA TNVGDEGGFA PNIQENKEGL ELLKTAIEKA GYTGKVVIGM 
       260        270        280        290        300
DVAASEFYND KDKTYDLNFK EENNDGSQKI SGDSLKNVYK SFVSEYPIVS 
       310        320        330        340        350
IEDPFDQDDW EHYAKMTAEI GEQVQIVGDD LLVTNPTRVA KAIQEKSCNA 
       360        370        380        390        400
LLLKVNQIGS VTESIEAVKM SKRAGWGVMT SHRSGETEDT FIADLAVGLA 
       410        420        430        440 
TGQIKTGAPC RSERLAKYNQ LLRIEEELGA AAVYAGAKFR APVEPY

Length:446

Mass (Da):47,973

Last modified:March 7, 2006 - v2

Checksum:ⁱBFCD813193800F1B

GO

Sequence cautionⁱ

The sequence AAN04181 differs from that shown. Reason: Erroneous gene model prediction.Curated

The sequence ABB46861 differs from that shown. Reason: Erroneous gene model prediction.Curated

The sequence ABB46862 differs from that shown. Reason: Erroneous gene model prediction.Curated

The sequence ABG65935 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	394	D → E in AAC49173 (Ref. 1) Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U09450 mRNA. Translation: AAC49173.1. AY335488 mRNA. Translation: AAP94211.1. AC131375 Genomic DNA. Translation: AAN04181.1. Sequence problems. DP000086 Genomic DNA. Translation: ABB46861.2. Sequence problems. DP000086 Genomic DNA. Translation: ABB46862.2. Sequence problems. DP000086 Genomic DNA. Translation: ABG65935.1. Sequence problems. AP008216 Genomic DNA. Translation: BAF26137.1. AP014966 Genomic DNA. No translation available. D17767 mRNA. Translation: BAA04612.1.
PIRⁱ	T03267.
NCBI Reference Sequences More...RefSeqⁱ	XP_015614256.1. XM_015758770.1.
UniGeneⁱ	Os.57462.

Genome annotation databases

GeneIDⁱ	4348176.
KEGGⁱ	osa:4348176.

Similar proteinsⁱ

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q42971	A1YQJ3 UPI0000EF7C07	Oryza sativa subsp. japonica (Rice)	446	UniRef100_Q42971	Cluster: Enolase	3

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q42971	A1YQJ3 UPI0000EF7C07 A0A0E0GPD6 A0A0E0QWR1 A0A0D3HBJ4 I1QSV0 A0A0E0GPD7 A0A0E0QWR3 A0A0E0GPD8 A0A0D3HBJ5 +32	Oryza sativa subsp. japonica (Rice) Oryza nivara (Indian wild rice) Oryza rufipogon (Brownbeard rice) (Asian wild rice) Oryza barthii Oryza glaberrima (African rice) Brachypodium distachyon (Purple false brome) (Trachynia distachya) Hordeum vulgare subsp. vulgare (Domesticated barley) Phleum pratense (Common timothy) Triticum aestivum (Wheat) Dichanthelium oligosanthes And more	446	UniRef90_Q42971	Cluster: Enolase	43

Entry	Cluster members	Organisms	Length	Cluster ID	Cluster name	Size
Q42971	P07323 UPI00042C2859 A0A1V4JAU6 UPI00042BE644 UPI000A1BB32D UPI000388D2A9 H9BVF0 P17183 Q545V3 D3Z2S4 +480	Rattus norvegicus (Rat) Chelonia mydas (Green sea-turtle) (Chelonia agassizi) Patagioenas fasciata monilis Odocoileus virginianus texanus Chrysemys picta bellii (Western painted turtle) (Emys bellii) Gekko japonicus (Schlegel's Japanese gecko) Mus musculus (Mouse) Heterocephalus glaber (Naked mole rat) Physeter catodon (Sperm whale) (Physeter macrocephalus) Gallus gallus (Chicken) And more	434	UniRef50_P07323	Cluster: Gamma-enolase	491

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U09450 mRNA. Translation: AAC49173.1. AY335488 mRNA. Translation: AAP94211.1. AC131375 Genomic DNA. Translation: AAN04181.1. Sequence problems. DP000086 Genomic DNA. Translation: ABB46861.2. Sequence problems. DP000086 Genomic DNA. Translation: ABB46862.2. Sequence problems. DP000086 Genomic DNA. Translation: ABG65935.1. Sequence problems. AP008216 Genomic DNA. Translation: BAF26137.1. AP014966 Genomic DNA. No translation available. D17767 mRNA. Translation: BAA04612.1.
PIRⁱ	T03267.
NCBI Reference Sequences More...RefSeqⁱ	XP_015614256.1. XM_015758770.1.
UniGeneⁱ	Os.57462.

3D structure databases

ProteinModelPortalⁱ	Q42971.
SMRⁱ	Q42971.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	39947.LOC_Os10g08550.1.

STRINGⁱ

39947.LOC_Os10g08550.1.

Proteomic databases

PaxDbⁱ	Q42971.
PRIDEⁱ	Q42971.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	4348176.
KEGGⁱ	osa:4348176.

Phylogenomic databases

eggNOGⁱ	KOG2670. Eukaryota. COG0148. LUCA.
InParanoidⁱ	Q42971.
KEGG Orthology (KO) More...KOⁱ	K01689.
Database of Orthologous Groups More...OrthoDBⁱ	EOG093608UK.

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00187.

UniPathwayⁱ

UPA00109; UER00187.

Gene expression databases

Genevisibleⁱ	Q42971. OS.

Genevisibleⁱ

Q42971. OS.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd03313. enolase. 1 hit.
Gene3Dⁱ	3.20.20.120. 1 hit. 3.30.390.10. 1 hit.
HAMAPⁱ	MF_00318. Enolase. 1 hit.
InterProⁱ	View protein in InterPro IPR000941. Enolase. IPR034390. Enolase-like_superfamily. IPR020810. Enolase_C. IPR029065. Enolase_C-like. IPR020809. Enolase_CS. IPR020811. Enolase_N. IPR029017. Enolase_N-like.
PANTHERⁱ	PTHR11902. PTHR11902. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00113. Enolase_C. 1 hit. PF03952. Enolase_N. 1 hit.
PIRSFⁱ	PIRSF001400. Enolase. 1 hit.
PRINTSⁱ	PR00148. ENOLASE.
SFLDⁱ	SFLDG00178. enolase. 1 hit. SFLDS00001. Enolase. 1 hit.
SMARTⁱ	View protein in SMART SM01192. Enolase_C. 1 hit. SM01193. Enolase_N. 1 hit.
SUPFAMⁱ	SSF51604. SSF51604. 1 hit. SSF54826. SSF54826. 1 hit.
TIGRFAMsⁱ	TIGR01060. eno. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00164. ENOLASE. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ENO_ORYSJ
Accessionⁱ	Q42971Primary (citable) accession number: Q42971 Secondary accession number(s): Q0IYS8 Q8LM12
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
	Last sequence update:	March 7, 2006
	Last modified:	August 30, 2017
	This is version 122 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
Oryza sativa (rice)
Index of Oryza sativa entries and their corresponding gene designations
SIMILARITY comments
Index of protein domains and families

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q42971 (ENO_ORYSJ)

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Enolase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ