Q42967 (DCUP_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uroporphyrinogen decarboxylase, chloroplastic
Short name=UPD
Short name=URO-D
EC=4.1.1.37

Gene names

Name:

DCUP

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	391 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III.
Catalytic activity	Uroporphyrinogen III = coproporphyrinogen + 4 CO2.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 4/4.
Subunit structure	Homodimer. Ref.2
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the uroporphyrinogen decarboxylase family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Decarboxylase Lyase
Technical term	3D-structure
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	uroporphyrinogen decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – ?

Chloroplast Potential

Chain

? – 391

Uroporphyrinogen decarboxylase, chloroplastic

PRO_0000036331

Regions

Region

71 – 75

5

Substrate binding By similarity

Sites

Binding site

90

1

Substrate By similarity

Binding site

120

1

Substrate By similarity

Binding site

121

1

Substrate By similarity

Binding site

198

1

Substrate By similarity

Binding site

253

1

Substrate By similarity

Binding site

368

1

Substrate By similarity

Site

121

1

Transition state stabilizer By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

391

Helix Strand Turn

Details...

Helix

52 – 58

7

Turn

74 – 76

3

Helix

79 – 85

7

Turn

90 – 95

6

Helix

97 – 111

15

Beta strand

114 – 117

4

Helix

124 – 129

6

Beta strand

133 – 136

4

Turn

137 – 139

3

Beta strand

140 – 145

6

Helix

150 – 155

6

Helix

161 – 164

4

Helix

166 – 179

14

Beta strand

182 – 190

9

Helix

192 – 201

10

Helix

209 – 217

9

Helix

219 – 242

24

Beta strand

246 – 251

6

Helix

253 – 257

5

Helix

260 – 266

7

Helix

268 – 281

14

Beta strand

287 – 290

4

Turn

295 – 297

3

Helix

298 – 304

7

Beta strand

307 – 310

4

Helix

317 – 323

7

Beta strand

326 – 331

6

Helix

336 – 340

5

Helix

343 – 357

15

Beta strand

359 – 364

6

Beta strand

366 – 368

3

Helix

376 – 387

12

Sequences

	Sequence		Length	Mass (Da)
	Q42967 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: ABDE69205918AE79	FASTA	391	43,116
	10 20 30 40 50 60 MMSCNYSFSS ISPSSKSAFT SPSNFNLNPR LICCSAGGTV AEPKAINATQ PLLLDAVRGK 70 80 90 100 110 120 EVERPPVWLM RQAGRYMKSY QLLCEKYPLF RDRSENVDLV VEISLQPWKV FRPDGVILFS 130 140 150 160 170 180 DILTPLSGMN IPFDIIKGKG PVIFDPLRTA ADVEKVREFI PEKSVPYVGE ALTILRKEVN 190 200 210 220 230 240 NQAAVLGFVG APFTLASYVV EGGSSKNFTK IKRLAFAEPK VLHALLQKFA TSMAKYIRYQ 250 260 270 280 290 300 ADSGAQAVQI FDSWATELSP VDFEEFSLPY LKQIVDSVKL THPNLPLILY ASGSGGLLER 310 320 330 340 350 360 LPLTGVDVVS LDWTVDMADG RRRLGPNVAI QGNVDPGVLF GSKEFITNRI NDTVKKAGKG 370 380 390 KHILNLGHGI KVGTPEENFA HFFEIAKGLR Y

References

[1]	"Isolation, sequencing and expression of cDNA sequences encoding uroporphyrinogen decarboxylase from tobacco and barley." Mock H.-P., Trainotti L., Kruse E., Grimm B. Plant Mol. Biol. 28:245-256(1995) [PubMed: 7599310] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. SR1. Tissue: Leaf.
[2]	"Crystal structure and substrate binding modeling of the uroporphyrinogen-III decarboxylase from Nicotiana tabacum. Implications for the catalytic mechanism." Martins B.M., Grimm B., Mock H.-P., Huber R., Messerschmidt A. J. Biol. Chem. 276:44108-44116(2001) [PubMed: 11524417] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 39-391, SUBUNIT, REACTION MECHANISM.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X82833 mRNA. Translation: CAA58040.1.

PIR

S55732.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1J93	X-ray	2.30	A	39-391	[»]

ProteinModelPortal

Q42967.

SMR

Q42967. Positions 49-391.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-11784.

Family and domain databases

InterPro

IPR006361. Uroporphyrinogen_deCO2ase_HemE.
IPR000257. Uroporphyrinogen_deCOase.
[Graphical view]

PANTHER

PTHR21091:SF2. HemE. 1 hit.

Pfam

PF01208. URO-D. 1 hit.
[Graphical view]

TIGRFAMs

TIGR01464. HemE. 1 hit.

PROSITE

PS00906. UROD_1. 1 hit.
PS00907. UROD_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DCUP_TOBAC

Accession

Primary (citable) accession number: Q42967

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	May 31, 2011
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program