ID   PMT1_TOBAC              Reviewed;         375 AA.
AC   Q42963; Q9SEH6;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   20-JAN-2009, entry version 55.
DE   RecName: Full=Putrescine N-methyltransferase 1;
DE            Short=PMT 1;
DE            EC=2.1.1.53;
DE   AltName: Full=A411;
GN   Name=PMT1;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Burley 21; TISSUE=Root;
RX   MEDLINE=94312878; PubMed=8038607; DOI=10.1105/tpc.6.5.723;
RA   Hibi N., Higashiguchi S., Hashimoto T., Yamada Y.;
RT   "Gene expression in tobacco low-nicotine mutants.";
RL   Plant Cell 6:723-735(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Xanthi;
RX   MEDLINE=20064975; PubMed=10598105; DOI=10.1023/A:1006342018991;
RA   Riechers D.E., Timko M.P.;
RT   "Structure and expression of the gene family encoding putrescine N-
RT   methyltransferase in Nicotiana tabacum: new clues to the evolutionary
RT   origin of cultivated tobacco.";
RL   Plant Mol. Biol. 41:387-401(1999).
CC   -!- CATALYTIC ACTIVITY: S-adenosyl-L-methionine + putrescine = S-
CC       adenosyl-L-homocysteine + N-methylputrescine.
CC   -!- PATHWAY: Alkaloid biosynthesis; nicotine biosynthesis.
CC   -!- TISSUE SPECIFICITY: Predominantly expressed in roots.
CC   -!- SIMILARITY: Belongs to the spermidine/spermine synthase family.
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DR   EMBL; D28506; BAA05867.1; -; mRNA.
DR   EMBL; AF126810; AAF14879.1; -; Genomic_DNA.
DR   PIR; T03681; T03681.
DR   HSSP; Q9WZC2; 1INL.
DR   SMR; Q42963; 83-374.
DR   BRENDA; 2.1.1.53; 298.
DR   GO; GO:0030750; F:putrescine N-methyltransferase activity; IEA:EC.
DR   InterPro; IPR001045; Sprmine_synthase.
DR   PANTHER; PTHR11558; Sprmine_synthase; 1.
DR   Pfam; PF01564; Spermine_synth; 1.
DR   TIGRFAMs; TIGR00417; speE; 1.
DR   PROSITE; PS01330; SPERMIDINE_SYNTHASE_1; 1.
DR   PROSITE; PS51006; SPERMIDINE_SYNTHASE_2; 1.
PE   2: Evidence at transcript level;
KW   Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN         1    375       Putrescine N-methyltransferase 1.
FT                                /FTId=PRO_0000156541.
FT   REGION      223    224       S-adenosyl-L-methionine binding (By
FT                                similarity).
FT   BINDING     117    117       S-adenosyl-L-methionine (By similarity).
FT   BINDING     192    192       S-adenosyl-L-methionine (By similarity).
FT   BINDING     242    242       S-adenosyl-L-methionine (By similarity).
FT   BINDING     311    311       S-adenosyl-L-methionine (By similarity).
FT   CONFLICT    308    308       A -> V (in Ref. 2; AAF14879).
SQ   SEQUENCE   375 AA;  41113 MW;  9E6483CDD4371287 CRC64;
     MEVISTNTNG STIFKNGAIP MNGHQNGTSE HLNGYQNGTS KHQNGHQNGT FEHRNGHQNG
     TSEQQNGTIS HDNGNELLGS SDSIKPGWFS EFSALWPGEA FSLKVEKLLF QGKSDYQDVM
     LFESATYGKV LTLDGAIQHT ENGGFPYTEM IVHLPLGSIP NPKKVLIIGG GIGFTLFEML
     RYPSIEKIDI VEIDDVVVDV SRKFFPYLAA NFNDPRVTLV LGDGAAFVKA AQAGYYDAII
     VDSSDPIGPA KDLFERPFFE AVAKALRPGG VVCTQAESIW LHMHIIKQII ANCRQVFKGS
     VNYAWTTAPT YPTGVIGYML CSTEGPEVDF KNPVNPIDKE TTQVKSKLGP LKFYNSDIHK
     AAFILPSFAR SMIES
//