Q42963 (PMT1_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putrescine N-methyltransferase 1
Short name=PMT 1
EC=2.1.1.53

Alternative name(s):
A411

Gene names

Name:

PMT1

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	375 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	S-adenosyl-L-methionine + putrescine = S-adenosyl-L-homocysteine + N-methylputrescine.
Pathway	Alkaloid biosynthesis; nicotine biosynthesis.
Tissue specificity	Predominantly expressed in roots.
Sequence similarities	Belongs to the spermidine/spermine synthase family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Molecular function	putrescine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 375	375	Putrescine N-methyltransferase 1		PRO_0000156541
Regions
	Region	223 – 224	2	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	242	1	Proton acceptor By similarity
	Binding site	117	1	S-adenosyl-L-methionine By similarity
	Binding site	192	1	S-adenosyl-L-methionine By similarity
	Binding site	311	1	S-adenosyl-L-methionine By similarity
Experimental info
	Sequence conflict	308	1	A → V in AAF14879. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q42963 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9E6483CDD4371287	FASTA	375	41,113
	10 20 30 40 50 60 MEVISTNTNG STIFKNGAIP MNGHQNGTSE HLNGYQNGTS KHQNGHQNGT FEHRNGHQNG 70 80 90 100 110 120 TSEQQNGTIS HDNGNELLGS SDSIKPGWFS EFSALWPGEA FSLKVEKLLF QGKSDYQDVM 130 140 150 160 170 180 LFESATYGKV LTLDGAIQHT ENGGFPYTEM IVHLPLGSIP NPKKVLIIGG GIGFTLFEML 190 200 210 220 230 240 RYPSIEKIDI VEIDDVVVDV SRKFFPYLAA NFNDPRVTLV LGDGAAFVKA AQAGYYDAII 250 260 270 280 290 300 VDSSDPIGPA KDLFERPFFE AVAKALRPGG VVCTQAESIW LHMHIIKQII ANCRQVFKGS 310 320 330 340 350 360 VNYAWTTAPT YPTGVIGYML CSTEGPEVDF KNPVNPIDKE TTQVKSKLGP LKFYNSDIHK 370 AAFILPSFAR SMIES

References

[1]	"Gene expression in tobacco low-nicotine mutants." Hibi N., Higashiguchi S., Hashimoto T., Yamada Y. Plant Cell 6:723-735(1994) [PubMed: 8038607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Burley 21. Tissue: Root.
[2]	"Structure and expression of the gene family encoding putrescine N-methyltransferase in Nicotiana tabacum: new clues to the evolutionary origin of cultivated tobacco." Riechers D.E., Timko M.P. Plant Mol. Biol. 41:387-401(1999) [PubMed: 10598105] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Xanthi.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D28506 mRNA. Translation: BAA05867.1. AF126810 Genomic DNA. Translation: AAF14879.1.
PIR	T03681.
3D structure databases
ProteinModelPortal	Q42963.
SMR	Q42963. Positions 83-374.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001045. Sprmine_synthase. [Graphical view]
PANTHER	PTHR11558. Sprmine_synthase. 1 hit.
Pfam	PF01564. Spermine_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00417. SpeE. 1 hit.
PROSITE	PS01330. SPERMIDINE_SYNTHASE_1. 1 hit. PS51006. SPERMIDINE_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMT1_TOBAC

Accession

Primary (citable) accession number: Q42963
Secondary accession number(s): Q9SEH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program