ID   PGKY_TOBAC              Reviewed;         401 AA.
AC   Q42962;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   27-MAR-2024, entry version 99.
DE   RecName: Full=Phosphoglycerate kinase, cytosolic;
DE            EC=2.7.2.3;
OS   Nicotiana tabacum (Common tobacco).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC   Nicotiana.
OX   NCBI_TaxID=4097;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Samsun; TISSUE=Leaf;
RA   Rao S.K., Bringloe D.H., Dyer T.A., Raines C.A., Bradbeer J.W.;
RT   "Nucleotide sequences of cDNAs encoding the chloroplastic and cytosolic
RT   phosphoglycerate kinases from tobacco.";
RL   (er) Plant Gene Register PGR95-090(1995).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl
CC         phosphate + ADP; Xref=Rhea:RHEA:14801, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:57604, ChEBI:CHEBI:58272, ChEBI:CHEBI:456216; EC=2.7.2.3;
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 2/5.
CC   -!- SUBUNIT: Monomer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the phosphoglycerate kinase family.
CC       {ECO:0000305}.
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DR   EMBL; Z48976; CAA88840.1; -; mRNA.
DR   PIR; T03661; T03661.
DR   RefSeq; NP_001312375.1; NM_001325446.1.
DR   AlphaFoldDB; Q42962; -.
DR   SMR; Q42962; -.
DR   STRING; 4097.Q42962; -.
DR   PaxDb; 4097-Q42962; -.
DR   ProMEX; Q42962; -.
DR   GeneID; 107787830; -.
DR   KEGG; nta:107787830; -.
DR   OMA; DYFVMDA; -.
DR   OrthoDB; 5477183at2759; -.
DR   PhylomeDB; Q42962; -.
DR   UniPathway; UPA00109; UER00185.
DR   Proteomes; UP000084051; Unplaced.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0043531; F:ADP binding; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IBA:GO_Central.
DR   GO; GO:0004618; F:phosphoglycerate kinase activity; IBA:GO_Central.
DR   GO; GO:0006094; P:gluconeogenesis; IBA:GO_Central.
DR   GO; GO:0006096; P:glycolytic process; IBA:GO_Central.
DR   GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR   CDD; cd00318; Phosphoglycerate_kinase; 1.
DR   Gene3D; 3.40.50.1260; Phosphoglycerate kinase, N-terminal domain; 2.
DR   HAMAP; MF_00145; Phosphoglyc_kinase; 1.
DR   InterPro; IPR001576; Phosphoglycerate_kinase.
DR   InterPro; IPR015911; Phosphoglycerate_kinase_CS.
DR   InterPro; IPR015824; Phosphoglycerate_kinase_N.
DR   InterPro; IPR036043; Phosphoglycerate_kinase_sf.
DR   PANTHER; PTHR11406; PHOSPHOGLYCERATE KINASE; 1.
DR   PANTHER; PTHR11406:SF27; PHOSPHOGLYCERATE KINASE 3, CYTOSOLIC; 1.
DR   Pfam; PF00162; PGK; 1.
DR   PIRSF; PIRSF000724; Pgk; 1.
DR   PRINTS; PR00477; PHGLYCKINASE.
DR   SUPFAM; SSF53748; Phosphoglycerate kinase; 1.
DR   PROSITE; PS00111; PGLYCERATE_KINASE; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Glycolysis; Kinase; Nucleotide-binding;
KW   Reference proteome; Transferase.
FT   CHAIN           1..401
FT                   /note="Phosphoglycerate kinase, cytosolic"
FT                   /id="PRO_0000145871"
FT   BINDING         25..27
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         41
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         64..67
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         122
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         155
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         206
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         297
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         328
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
FT   BINDING         357..360
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   401 AA;  42364 MW;  4AD69E74F8F4CDED CRC64;
     MAVKKSVGSL KEADLKGKRV FVRVDLNVPL DENFNITDDT RIRAAVPTIK YLMQHGSHVI
     LASHLGRPKG VTPKYSLKPL VPRLSELLGV EVKIANDSIG PEVEKLVAEI PEGGVLLLEN
     VRFYKEEEKN EPEFAKKLAS LADLYVNDAF GTAHRAHAST EGVAKYLKPA VAGFLMQKEL
     DYLVGAVANP QKPFAAIVGG SKVSSKIGVI ESLLEKVDVL LLGGGMIFTF YKAQGYAVGS
     SLVEEDKLDL ATSLMEKAKA KGVSLLLPTD VVIADKFAAD ANSKVVPASE IPDGWMGLDI
     GPDAIKSFGS ALDTTKTVIW NGPMGVFEFD KFAAGTEAIA KKLAELSGKG VTTIIGGGDS
     VAAVEKVGLA EKMSHISTGG GASLELLEGK PLPGVLALDD A
//