Reviewed, UniProtKB/Swiss-Prot Q42962 (PGKY_TOBAC)

Names and origin

Protein names	Recommended name:     Phosphoglycerate kinase, cytosolic     EC=2.7.2.3
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	401 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
Subunit structure	Monomer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 401	401	Phosphoglycerate kinase, cytosolic		PRO_0000145871
Regions
	Nucleotide binding	357 – 360	4	ATP By similarity
	Region	25 – 27	3	Substrate binding By similarity
	Region	64 – 67	4	Substrate binding By similarity
Sites
	Binding site	41	1	Substrate By similarity
	Binding site	122	1	Substrate By similarity
	Binding site	155	1	Substrate By similarity
	Binding site	206	1	ATP By similarity
	Binding site	297	1	ATP; via carbonyl oxygen By similarity
	Binding site	328	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42962-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 4AD69E74F8F4CDED	FASTA	401	42,364
	10 20 30 40 50 60 MAVKKSVGSL KEADLKGKRV FVRVDLNVPL DENFNITDDT RIRAAVPTIK YLMQHGSHVI 70 80 90 100 110 120 LASHLGRPKG VTPKYSLKPL VPRLSELLGV EVKIANDSIG PEVEKLVAEI PEGGVLLLEN 130 140 150 160 170 180 VRFYKEEEKN EPEFAKKLAS LADLYVNDAF GTAHRAHAST EGVAKYLKPA VAGFLMQKEL 190 200 210 220 230 240 DYLVGAVANP QKPFAAIVGG SKVSSKIGVI ESLLEKVDVL LLGGGMIFTF YKAQGYAVGS 250 260 270 280 290 300 SLVEEDKLDL ATSLMEKAKA KGVSLLLPTD VVIADKFAAD ANSKVVPASE IPDGWMGLDI 310 320 330 340 350 360 GPDAIKSFGS ALDTTKTVIW NGPMGVFEFD KFAAGTEAIA KKLAELSGKG VTTIIGGGDS 370 380 390 400 VAAVEKVGLA EKMSHISTGG GASLELLEGK PLPGVLALDD A

References

[1]	"Nucleotide sequences of cDNAs encoding the chloroplastic and cytosolic phosphoglycerate kinases from tobacco." Rao S.K., Bringloe D.H., Dyer T.A., Raines C.A., Bradbeer J.W. Plant Gene Register PGR95-090 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Samsun. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z48976 mRNA. Translation: CAA88840.1.
PIR	T03661.
3D structure databases
HSSP	HSSP built from PDB template 1PHP based on UniProtKB P18912.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.2.3. 298.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKY_TOBAC

Accession

Primary (citable) accession number: Q42962

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)