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Protein

Phosphoglycerate kinase, cytosolic

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.

Pathwayi: glycolysis

This protein is involved in step 2 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC)
  2. Phosphoglycerate kinase, cytosolic
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  4. no protein annotated in this organism
  5. Pyruvate kinase isozyme G, chloroplastic, Pyruvate kinase, Pyruvate kinase isozyme A, chloroplastic, Pyruvate kinase, cytosolic isozyme, Pyruvate kinase (PKTL7)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41SubstrateBy similarity1
Binding sitei122SubstrateBy similarity1
Binding sitei155SubstrateBy similarity1
Binding sitei206ATPBy similarity1
Binding sitei297ATP; via carbonyl oxygenBy similarity1
Binding sitei328ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi357 – 360ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoglycerate kinase, cytosolic (EC:2.7.2.3)
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001458711 – 401Phosphoglycerate kinase, cytosolicAdd BLAST401

Proteomic databases

PRIDEiQ42962.
ProMEXiQ42962.

Interactioni

Subunit structurei

Monomer.

Structurei

3D structure databases

ProteinModelPortaliQ42962.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni25 – 27Substrate bindingBy similarity3
Regioni64 – 67Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the phosphoglycerate kinase family.Curated

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42962-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVKKSVGSL KEADLKGKRV FVRVDLNVPL DENFNITDDT RIRAAVPTIK
60 70 80 90 100
YLMQHGSHVI LASHLGRPKG VTPKYSLKPL VPRLSELLGV EVKIANDSIG
110 120 130 140 150
PEVEKLVAEI PEGGVLLLEN VRFYKEEEKN EPEFAKKLAS LADLYVNDAF
160 170 180 190 200
GTAHRAHAST EGVAKYLKPA VAGFLMQKEL DYLVGAVANP QKPFAAIVGG
210 220 230 240 250
SKVSSKIGVI ESLLEKVDVL LLGGGMIFTF YKAQGYAVGS SLVEEDKLDL
260 270 280 290 300
ATSLMEKAKA KGVSLLLPTD VVIADKFAAD ANSKVVPASE IPDGWMGLDI
310 320 330 340 350
GPDAIKSFGS ALDTTKTVIW NGPMGVFEFD KFAAGTEAIA KKLAELSGKG
360 370 380 390 400
VTTIIGGGDS VAAVEKVGLA EKMSHISTGG GASLELLEGK PLPGVLALDD

A
Length:401
Mass (Da):42,364
Last modified:November 1, 1996 - v1
Checksum:i4AD69E74F8F4CDED
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48976 mRNA. Translation: CAA88840.1.
PIRiT03661.
RefSeqiNP_001312375.1. NM_001325446.1.
UniGeneiNta.7278.

Genome annotation databases

GeneIDi107787830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48976 mRNA. Translation: CAA88840.1.
PIRiT03661.
RefSeqiNP_001312375.1. NM_001325446.1.
UniGeneiNta.7278.

3D structure databases

ProteinModelPortaliQ42962.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ42962.
ProMEXiQ42962.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107787830.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00185.

Family and domain databases

CDDicd00318. Phosphoglycerate_kinase. 1 hit.
Gene3Di3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPiMF_00145. Phosphoglyc_kinase. 1 hit.
InterProiIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERiPTHR11406. PTHR11406. 1 hit.
PfamiPF00162. PGK. 1 hit.
[Graphical view]
PIRSFiPIRSF000724. Pgk. 1 hit.
PRINTSiPR00477. PHGLYCKINASE.
SUPFAMiSSF53748. SSF53748. 1 hit.
PROSITEiPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPGKY_TOBAC
AccessioniPrimary (citable) accession number: Q42962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 30, 2016
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.