Reviewed, UniProtKB/Swiss-Prot Q42961 (PGKH_TOBAC)

Names and origin

Protein names	Recommended name:     Phosphoglycerate kinase, chloroplastic     EC=2.7.2.3
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: InterPro reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoglycerate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 75	75	Chloroplast Potential
	Chain	76 – 481	406	Phosphoglycerate kinase, chloroplastic		PRO_0000023892
Regions
	Nucleotide binding	431 – 434	4	ATP By similarity
	Region	99 – 101	3	Substrate binding By similarity
	Region	138 – 141	4	Substrate binding By similarity
Sites
	Binding site	115	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	229	1	Substrate By similarity
	Binding site	280	1	ATP By similarity
	Binding site	371	1	ATP; via carbonyl oxygen By similarity
	Binding site	402	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42961-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7AA497C747D0716D	FASTA	481	50,177
	10 20 30 40 50 60 MASATASHTL CGIPATSSST TNKAIAPSSA RFLAKTPLRR LGFAGAAADS LFTNHVATKL 70 80 90 100 110 120 RSLKSSSKPI RGVASMAKKS VGDLTAAELK GKKVFVRADL NVPLDDNQNI TDDTRIRAAV 130 140 150 160 170 180 PTIKHLMANG AKVILSSHLG RPKGVTPKYS LAPLVPRLSE LLGIQVVKVE DCIGPEVEKL 190 200 210 220 230 240 VASLPEGGVL LLENVRFYKE EEKNEPEFAK KLASLADLYV NDAFGTAHRA HASTEGVTKF 250 260 270 280 290 300 LKPSVAGFLL QKELDYLVGA VSNPKRPFAA IVGGSKVSSK IGVIESLLEK CDILLLGGGM 310 320 330 340 350 360 IFTFYKAQGL SVGSSLVEED KLELATSLLE KAKAKGVSLL LPSDVVIADK FAPDANSKIV 370 380 390 400 410 420 PASAIPDGWM GLDIGPDSVK TFNDALDTTK TVIWNGPMGV FEFDKFAVGT EAIAKKLADL 430 440 450 460 470 480 SGKGVTTIIG GGDSVAAVEK VGVASVMSHI STGGGASLEL LEGKVLPGVI ALDEADAPVA V

References

[1]	"Nucleotide sequences of cDNAs encoding the chloroplastic and cytosolic phosphoglycerate kinases from tobacco." Rao S.K., Bringloe D.H., Dyer T.A., Raines C.A., Bradbeer J.W. Plant Gene Register PGR95-090 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Samsun. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z48977 mRNA. Translation: CAA88841.1.
PIR	T03660.
3D structure databases
SMR	Q42961. Positions 76-474.
ModBase	Search...
Proteomic databases
PRIDE	Q42961.
Enzyme and pathway databases
BRENDA	2.7.2.3. 298.
Family and domain databases
InterPro	IPR001576. Phosphoglycerate_kinase. IPR015901. Phosphoglycerate_kinase_C. IPR015911. Phosphoglycerate_kinase_CS. IPR015824. Phosphoglycerate_kinase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.1270. Phosphoglycerate_kinase_C. 1 hit. G3DSA:3.40.50.1260. Phosphoglycerate_kinase_N. 1 hit.
PANTHER	PTHR11406. PGK. 1 hit.
Pfam	PF00162. PGK. 1 hit. [Graphical view]
PRINTS	PR00477. PHGLYCKINASE.
PROSITE	PS00111. PGLYCERATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGKH_TOBAC

Accession

Primary (citable) accession number: Q42961

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	January 19, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)