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Protein

Pyruvate kinase, cytosolic isozyme

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase, cytosolic (GAPC)
  2. Phosphoglycerate kinase, cytosolic
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
  4. no protein annotated in this organism
  5. Pyruvate kinase (LOC107803087), Pyruvate kinase (LOC107824278), Pyruvate kinase (LOC107761670), Pyruvate kinase (LOC107807979), Pyruvate kinase (LOC107830802), Pyruvate kinase (LOC107830802), Pyruvate kinase, cytosolic isozyme, Pyruvate kinase (LOC107787533), Pyruvate kinase (LOC107797877), Pyruvate kinase (LOC107778911), Pyruvate kinase (LOC107821513), Pyruvate kinase (LOC107830388), Pyruvate kinase (LOC107820699), Pyruvate kinase (LOC107830388), Pyruvate kinase (LOC107822892), Pyruvate kinase (LOC107783505), Pyruvate kinase (LOC107827388), Pyruvate kinase (LOC107777547), Pyruvate kinase isozyme G, chloroplastic, Pyruvate kinase, Pyruvate kinase isozyme A, chloroplastic, Pyruvate kinase (LOC107799668), Pyruvate kinase (LOC107773422), Pyruvate kinase (LOC107787620), Pyruvate kinase (LOC107818508), Pyruvate kinase (LOC107760370), Pyruvate kinase (PKTL7), Pyruvate kinase (LOC107782907), Pyruvate kinase (LOC107818508)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei48SubstrateBy similarity1
Metal bindingi50PotassiumBy similarity1
Metal bindingi52PotassiumBy similarity1
Metal bindingi82PotassiumBy similarity1
Metal bindingi83Potassium; via carbonyl oxygenBy similarity1
Sitei238Transition state stabilizerBy similarity1
Metal bindingi240MagnesiumSequence analysis1
Binding sitei263Substrate; via amide nitrogenBy similarity1
Metal bindingi264MagnesiumBy similarity1
Binding sitei264Substrate; via amide nitrogenBy similarity1
Binding sitei296SubstrateBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase, cytosolic isozyme (EC:2.7.1.40)
Short name:
PK
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana
Proteomesi
  • UP000084051 Componenti: Genome assembly

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121271 – 508Pyruvate kinase, cytosolic isozymeAdd BLAST508

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ42954.
SMRiQ42954.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

KOiK00873.

Family and domain databases

CDDicd00288. Pyruvate_Kinase. 1 hit.
Gene3Di2.40.33.10. 1 hit.
3.40.1380.20. 1 hit.
InterProiView protein in InterPro
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom_sf.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR036918. Pyrv_Knase_C_sf.
IPR015806. Pyrv_Knase_insert_dom_sf.
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiView protein in Pfam
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiView protein in PROSITE
PS00110. PYRUVATE_KINASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q42954-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIENNNNGV NFCTVKRPKT KIVCTLGPAS RSVPMIEKLL RAGMNVARFN
60 70 80 90 100
FSHGSHDYHQ ETIDNLRQAM ESTGILCAVM LDTKGPEIRT GFLKDAKPVQ
110 120 130 140 150
LKQGQEITIS TDYSIKGDES MICMSYKKLA EDVKPQSVIL CADGQITFTV
160 170 180 190 200
LSCDKENGLD RCRCENTAVL GERKNVNLPG VIVDLPTLTD KDKDDILNWG
210 220 230 240 250
VPNHIDMIAL SFVRKGSDLV EVRKLLGEHA KNILLMSKVE NQEGVANFDD
260 270 280 290 300
ILLNSDAFMV ARGDLGMEIP IEKIFLAQKV MIYKCNIQGK PVVTATQMLE
310 320 330 340 350
SMIKSPRPTR AEATDVANAV LDGTDCVMLS GETAAGAYPD LAVGTMAKIC
360 370 380 390 400
IEAESTIDYP DVFKRIMSNA PVPMSPLESL ASSAVRTANS AKAALILVLT
410 420 430 440 450
RGGSTAKLVA KYRPGMPILS VVVPEIKTDS FDWTCSDESP ARHSLIFRGL
460 470 480 490 500
VPVLHAGSAR ASHEESTEEA LDFALQHAKT KGLCKQGDSV VALHRVGTAS

VIKIVTVK
Length:508
Mass (Da):55,133
Last modified:November 1, 1996 - v1
Checksum:iA8FC5DA9D7CB9989
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z29492 mRNA. Translation: CAA82628.1.
PIRiS41379.
RefSeqiNP_001312569.1. NM_001325640.1.
UniGeneiNta.4194.

Genome annotation databases

GeneIDi107797877.
KEGGinta:107797877.

Similar proteinsi

Entry informationi

Entry nameiKPYC_TOBAC
AccessioniPrimary (citable) accession number: Q42954
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: February 28, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome