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Q42954 (KPYC_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase, cytosolic isozyme

Short name=PK
EC=2.7.1.40
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length508 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the pyruvate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Magnesium
Metal-binding
Nucleotide-binding
Potassium
Pyruvate
   Molecular functionKinase
Transferase
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

magnesium ion binding

Inferred from electronic annotation. Source: InterPro

potassium ion binding

Inferred from electronic annotation. Source: InterPro

pyruvate kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 508508Pyruvate kinase, cytosolic isozyme
PRO_0000112127

Sites

Metal binding501Potassium By similarity
Metal binding521Potassium By similarity
Metal binding821Potassium By similarity
Metal binding831Potassium; via carbonyl oxygen By similarity
Metal binding2401Magnesium Potential
Metal binding2641Magnesium By similarity
Binding site481Substrate By similarity
Binding site2631Substrate; via amide nitrogen By similarity
Binding site2641Substrate; via amide nitrogen By similarity
Binding site2961Substrate By similarity
Site2381Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q42954 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: A8FC5DA9D7CB9989

FASTA50855,133
        10         20         30         40         50         60 
MAIENNNNGV NFCTVKRPKT KIVCTLGPAS RSVPMIEKLL RAGMNVARFN FSHGSHDYHQ 

        70         80         90        100        110        120 
ETIDNLRQAM ESTGILCAVM LDTKGPEIRT GFLKDAKPVQ LKQGQEITIS TDYSIKGDES 

       130        140        150        160        170        180 
MICMSYKKLA EDVKPQSVIL CADGQITFTV LSCDKENGLD RCRCENTAVL GERKNVNLPG 

       190        200        210        220        230        240 
VIVDLPTLTD KDKDDILNWG VPNHIDMIAL SFVRKGSDLV EVRKLLGEHA KNILLMSKVE 

       250        260        270        280        290        300 
NQEGVANFDD ILLNSDAFMV ARGDLGMEIP IEKIFLAQKV MIYKCNIQGK PVVTATQMLE 

       310        320        330        340        350        360 
SMIKSPRPTR AEATDVANAV LDGTDCVMLS GETAAGAYPD LAVGTMAKIC IEAESTIDYP 

       370        380        390        400        410        420 
DVFKRIMSNA PVPMSPLESL ASSAVRTANS AKAALILVLT RGGSTAKLVA KYRPGMPILS 

       430        440        450        460        470        480 
VVVPEIKTDS FDWTCSDESP ARHSLIFRGL VPVLHAGSAR ASHEESTEEA LDFALQHAKT 

       490        500 
KGLCKQGDSV VALHRVGTAS VIKIVTVK 

« Hide

References

[1]"Molecular characterization of plastid pyruvate kinase from castor and tobacco."
Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z29492 mRNA. Translation: CAA82628.1.
PIRS41379.

3D structure databases

ProteinModelPortalQ42954.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYC_TOBAC
AccessionPrimary (citable) accession number: Q42954
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: June 11, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways