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Q42954

- KPYC_TOBAC

UniProt

Q42954 - KPYC_TOBAC

Protein

Pyruvate kinase, cytosolic isozyme

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + pyruvate = ADP + phosphoenolpyruvate.

    Cofactori

    Magnesium.By similarity
    Potassium.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei48 – 481SubstrateBy similarity
    Metal bindingi50 – 501PotassiumBy similarity
    Metal bindingi52 – 521PotassiumBy similarity
    Metal bindingi82 – 821PotassiumBy similarity
    Metal bindingi83 – 831Potassium; via carbonyl oxygenBy similarity
    Sitei238 – 2381Transition state stabilizerBy similarity
    Metal bindingi240 – 2401MagnesiumSequence Analysis
    Binding sitei263 – 2631Substrate; via amide nitrogenBy similarity
    Metal bindingi264 – 2641MagnesiumBy similarity
    Binding sitei264 – 2641Substrate; via amide nitrogenBy similarity
    Binding sitei296 – 2961SubstrateBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. magnesium ion binding Source: InterPro
    3. potassium ion binding Source: InterPro
    4. pyruvate kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00188.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pyruvate kinase, cytosolic isozyme (EC:2.7.1.40)
    Short name:
    PK
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 508508Pyruvate kinase, cytosolic isozymePRO_0000112127Add
    BLAST

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ42954.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the pyruvate kinase family.Curated

    Family and domain databases

    Gene3Di2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProiIPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view]
    PANTHERiPTHR11817. PTHR11817. 1 hit.
    PfamiPF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view]
    PRINTSiPR01050. PYRUVTKNASE.
    SUPFAMiSSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
    PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q42954-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAIENNNNGV NFCTVKRPKT KIVCTLGPAS RSVPMIEKLL RAGMNVARFN    50
    FSHGSHDYHQ ETIDNLRQAM ESTGILCAVM LDTKGPEIRT GFLKDAKPVQ 100
    LKQGQEITIS TDYSIKGDES MICMSYKKLA EDVKPQSVIL CADGQITFTV 150
    LSCDKENGLD RCRCENTAVL GERKNVNLPG VIVDLPTLTD KDKDDILNWG 200
    VPNHIDMIAL SFVRKGSDLV EVRKLLGEHA KNILLMSKVE NQEGVANFDD 250
    ILLNSDAFMV ARGDLGMEIP IEKIFLAQKV MIYKCNIQGK PVVTATQMLE 300
    SMIKSPRPTR AEATDVANAV LDGTDCVMLS GETAAGAYPD LAVGTMAKIC 350
    IEAESTIDYP DVFKRIMSNA PVPMSPLESL ASSAVRTANS AKAALILVLT 400
    RGGSTAKLVA KYRPGMPILS VVVPEIKTDS FDWTCSDESP ARHSLIFRGL 450
    VPVLHAGSAR ASHEESTEEA LDFALQHAKT KGLCKQGDSV VALHRVGTAS 500
    VIKIVTVK 508
    Length:508
    Mass (Da):55,133
    Last modified:November 1, 1996 - v1
    Checksum:iA8FC5DA9D7CB9989
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z29492 mRNA. Translation: CAA82628.1.
    PIRiS41379.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z29492 mRNA. Translation: CAA82628.1 .
    PIRi S41379.

    3D structure databases

    ProteinModelPortali Q42954.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00188 .

    Family and domain databases

    Gene3Di 2.40.33.10. 1 hit.
    3.20.20.60. 2 hits.
    3.40.1380.20. 1 hit.
    InterProi IPR001697. Pyr_Knase.
    IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
    IPR011037. Pyrv_Knase-like_insert_dom.
    IPR015794. Pyrv_Knase_a/b.
    IPR018209. Pyrv_Knase_AS.
    IPR015793. Pyrv_Knase_brl.
    IPR015795. Pyrv_Knase_C.
    IPR015806. Pyrv_Knase_insert_dom.
    [Graphical view ]
    PANTHERi PTHR11817. PTHR11817. 1 hit.
    Pfami PF00224. PK. 1 hit.
    PF02887. PK_C. 1 hit.
    [Graphical view ]
    PRINTSi PR01050. PYRUVTKNASE.
    SUPFAMi SSF50800. SSF50800. 1 hit.
    SSF51621. SSF51621. 2 hits.
    SSF52935. SSF52935. 1 hit.
    TIGRFAMsi TIGR01064. pyruv_kin. 1 hit.
    PROSITEi PS00110. PYRUVATE_KINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of plastid pyruvate kinase from castor and tobacco."
      Blakeley S.D., Gottlob-Mchugh S., Wan J., Crews L., Miki B., Ko K., Dennis D.T.
      Plant Mol. Biol. 27:79-89(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiKPYC_TOBAC
    AccessioniPrimary (citable) accession number: Q42954
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3