Q42942 (UMPS_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uridine 5'-monophosphate synthase
Short name=UMP synthase

Including the following 2 domains:

1. Orotate phosphoribosyltransferase
   Short name=OPRTase
   EC=2.4.2.10
2. Orotidine 5'-phosphate decarboxylase
   EC=4.1.1.23
   Alternative name(s):
   OMPdecase

Gene names

Name:

PYR5-6

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	461 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Orotidine 5'-phosphate = UMP + CO2.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. In the C-terminal section; belongs to the OMP decarboxylase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Glycosyltransferase Lyase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: InterPro 'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	orotate phosphoribosyltransferase activity Inferred from electronic annotation. Source: EC orotidine-5'-phosphate decarboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 461	›461	Uridine 5'-monophosphate synthase		PRO_0000139654
Sites
	Active site	291	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q42942 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: AA96479701957CD1	FASTA	461	49,761
	10 20 30 40 50 60 DISAVKFGEF KLKSGISSPI YIDLRLIVSY PSILRQISQT LVGSLPSSTK YDVVCGVPYT 70 80 90 100 110 120 ALPIATCIST AHDVPMLMRR KEVKDYGTAK AIEGAFKPGQ ACLIVEDLVT SGASVLETAA 130 140 150 160 170 180 PLRAAGLTVT DAVVMIDREQ GGRENLAENG ITLHSMVKLT EMVKILKEKG RVSEETEKMV 190 200 210 220 230 240 KKFLEENRKV AVPVKETKVS LRLPYQERAK IAKNPTGKKL FEIMVQKETN LCLSADVATA 250 260 270 280 290 300 AELLDIADKV GPEICMLKTH VDILPDFTPD FGSKLRSIAD KHNFLIFEDR KFADIGNTVT 310 320 330 340 350 360 MQYEGGIFRI LDWADITNAH IISGPGIVDG LKLKGLSRGR GLLLLAEMSS AGNLATGAYT 370 380 390 400 410 420 AAAVKIAEDH SDFVIGFISV NPASWPNAPG NPSLIHATPG VQLVKGGDAL GQLYNTPSAV 430 440 450 460 IADRGSDIII VGRGIIKAAN PIEAAREYRL QGWDAYLVNC K

References

[1]	"Nucleotide sequence of a cDNA encoding UMP synthase from Nicotiana tabacum." Maier T., Zhou L., Thornburg R.W. Plant Gene Register PGR95-025 Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. SR1. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U22260 mRNA. Translation: AAC49115.1.
PIR	T02058.
3D structure databases
ProteinModelPortal	Q42942.
SMR	Q42942. Positions 203-457.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase. IPR018089. OMPdecase_AS. IPR001754. OMPdeCOase_dom. IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF00215. OMPdecase. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
SMART	SM00934. OMPdecase. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00336. PyrE. 1 hit. TIGR01740. PyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UMPS_TOBAC

Accession

Primary (citable) accession number: Q42942

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 21, 2011
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program