Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi: pentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform
  2. no protein annotated in this organism
  3. 6-phosphogluconate dehydrogenase, decarboxylating
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei71NADPBy similarity1
Binding sitei182NADP; via carbonyl oxygenBy similarity1
Binding sitei182SubstrateBy similarity1
Binding sitei250SubstrateBy similarity1
Binding sitei269SubstrateBy similarity1
Active sitei274Proton acceptorBy similarity1
Binding sitei360SubstrateBy similarity1
Binding sitei365SubstrateBy similarity1
Binding sitei396SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi36 – 43NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processCarbohydrate metabolism, Glucose metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform (EC:1.1.1.49)
Short name:
G6PD
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000681001 – 515Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoformAdd BLAST515

Proteomic databases

PRIDEiQ42919.

Structurei

3D structure databases

ProteinModelPortaliQ42919.
SMRiQ42919.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni212 – 216Substrate bindingBy similarity5

Sequence similaritiesi

Family and domain databases

HAMAPiMF_00966. G6PD. 1 hit.
InterProiView protein in InterPro
IPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiView protein in Pfam
PF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiView protein in PROSITE
PS00069. G6P_DEHYDROGENASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q42919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTNEWHVER RDSIGTESPV AREVLETGTL SIVVLGASGD LAKKKTFPAL
60 70 80 90 100
FHLYKQELLP PDEVHIFGYA RSKISDDELR NKLRSYLVPE KGASPKQLDD
110 120 130 140 150
VSKFLQLVKY VSGPYDSEDG FRLLDKEISE HEYLKNSKEG SSRRLFYLAL
160 170 180 190 200
PPSVYPSVCK MIKTCCMNKS DLGGWTRVVV EKPFGRDLES AEELSTQIGE
210 220 230 240 250
LFEEPQIYRI DHYLGKELVQ NMLVLRFANR FFLPLWNHNH IDNVQIVFRE
260 270 280 290 300
DFGTDGRGGY FDQYGIIRDI IPNHLLQVLC LIAMEKPVSL KPEHIRDEKV
310 320 330 340 350
KVLESVLPIR DDEVVLGQYE GYTDDPTVPD DSNTPTFATT ILRIHNERWE
360 370 380 390 400
GVPFIVKAGK ALNSRKAEIR VQFKDVPGDI FRSKKQGRNE FVIRLQPSEA
410 420 430 440 450
IYMKLTVKQP GLEMSAVQSE LDLSYGQRYQ GITIPEAYER LILDTIRGDQ
460 470 480 490 500
QHFVRRDELK ASWQIFTPLL HKIDRGELKP VPYNPGSRGP AEADELLEKA
510
GYVQTPGYIW IPPTL
Length:515
Mass (Da):58,923
Last modified:November 1, 1996 - v1
Checksum:i836C1B96F2709FC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18238 mRNA. Translation: AAB41552.1.
PIRiS57785.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiG6PD_MEDSA
AccessioniPrimary (citable) accession number: Q42919
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: May 10, 2017
This is version 90 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families