Reviewed, UniProtKB/Swiss-Prot Q42919 (G6PD_MEDSA)

Names and origin

Protein names	Recommended name:     Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform       Short name=G6PD     EC=1.1.1.49
Organism	Medicago sativa (Alfalfa)
Taxonomic identifier	3879 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Trifolieae › Medicago

Protein attributes

Sequence length	515 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glucose 6-phosphate + NADP(+) = D-glucono-1,5-lactone 6-phosphate + NADPH.
Pathway	Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro glucose-6-phosphate dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 515	515	Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform		PRO_0000068100
Sites
	Active site	274	1	Proton acceptor By similarity
	Binding site	38	1	NADP By similarity
	Binding site	71	1	NADP By similarity
	Binding site	212	1	Substrate By similarity
	Binding site	216	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42919-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 836C1B96F2709FC7	FASTA	515	58,923
	10 20 30 40 50 60 MGTNEWHVER RDSIGTESPV AREVLETGTL SIVVLGASGD LAKKKTFPAL FHLYKQELLP 70 80 90 100 110 120 PDEVHIFGYA RSKISDDELR NKLRSYLVPE KGASPKQLDD VSKFLQLVKY VSGPYDSEDG 130 140 150 160 170 180 FRLLDKEISE HEYLKNSKEG SSRRLFYLAL PPSVYPSVCK MIKTCCMNKS DLGGWTRVVV 190 200 210 220 230 240 EKPFGRDLES AEELSTQIGE LFEEPQIYRI DHYLGKELVQ NMLVLRFANR FFLPLWNHNH 250 260 270 280 290 300 IDNVQIVFRE DFGTDGRGGY FDQYGIIRDI IPNHLLQVLC LIAMEKPVSL KPEHIRDEKV 310 320 330 340 350 360 KVLESVLPIR DDEVVLGQYE GYTDDPTVPD DSNTPTFATT ILRIHNERWE GVPFIVKAGK 370 380 390 400 410 420 ALNSRKAEIR VQFKDVPGDI FRSKKQGRNE FVIRLQPSEA IYMKLTVKQP GLEMSAVQSE 430 440 450 460 470 480 LDLSYGQRYQ GITIPEAYER LILDTIRGDQ QHFVRRDELK ASWQIFTPLL HKIDRGELKP 490 500 510 VPYNPGSRGP AEADELLEKA GYVQTPGYIW IPPTL

References

[1]

"Stress responses in alfalfa (Medicago sativa L.) XIX. Transcriptional activation of oxidative pentose phosphate pathway genes at the onset of the isoflavonoid phytoalexin response." Fahrendorf T., Ni W., Shorrosh B.S., Dixon R.A. Plant Mol. Biol. 28:885-900(1995) [PubMed: 7640360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Apollo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U18238 mRNA. Translation: AAB41552.1.
PIR	S57785.
3D structure databases
HSSP	HSSP built from PDB template 1QKI based on UniProtKB P11413.
ModBase	Search...
Family and domain databases
InterPro	IPR001282. Glc-6-P_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR23429. G6PDH. 1 hit.
Pfam	PF02781. G6PD_C. 1 hit. PF00479. G6PD_N. 1 hit. [Graphical view]
PIRSF	PIRSF000110. G6PD. 1 hit.
PRINTS	PR00079. G6PDHDRGNASE.
ProDom	PD001129. G6PD. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00871. zwf. 1 hit.
PROSITE	PS00069. G6P_DEHYDROGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G6PD_MEDSA

Accession

Primary (citable) accession number: Q42919

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	November 25, 2008
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)