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Q42919 (G6PD_MEDSA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform

Short name=G6PD
EC=1.1.1.49
OrganismMedicago sativa (Alfalfa)
Taxonomic identifier3879 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Protein attributes

Sequence length515 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis By similarity. HAMAP-Rule MF_00966

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. HAMAP-Rule MF_00966

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subcellular location

Cytoplasm HAMAP-Rule MF_00966.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
   Cellular componentCytoplasm
   LigandNADP
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processpentose-phosphate shunt

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionNADP binding

Inferred from electronic annotation. Source: InterPro

glucose-6-phosphate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 515515Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform HAMAP-Rule MF_00966
PRO_0000068100

Regions

Nucleotide binding36 – 438NADP By similarity
Region212 – 2165Substrate binding By similarity

Sites

Active site2741Proton acceptor By similarity
Binding site711NADP By similarity
Binding site1821NADP; via carbonyl oxygen By similarity
Binding site1821Substrate By similarity
Binding site2501Substrate By similarity
Binding site2691Substrate By similarity
Binding site3601Substrate By similarity
Binding site3651Substrate By similarity
Binding site3961Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q42919 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 836C1B96F2709FC7

FASTA51558,923
        10         20         30         40         50         60 
MGTNEWHVER RDSIGTESPV AREVLETGTL SIVVLGASGD LAKKKTFPAL FHLYKQELLP 

        70         80         90        100        110        120 
PDEVHIFGYA RSKISDDELR NKLRSYLVPE KGASPKQLDD VSKFLQLVKY VSGPYDSEDG 

       130        140        150        160        170        180 
FRLLDKEISE HEYLKNSKEG SSRRLFYLAL PPSVYPSVCK MIKTCCMNKS DLGGWTRVVV 

       190        200        210        220        230        240 
EKPFGRDLES AEELSTQIGE LFEEPQIYRI DHYLGKELVQ NMLVLRFANR FFLPLWNHNH 

       250        260        270        280        290        300 
IDNVQIVFRE DFGTDGRGGY FDQYGIIRDI IPNHLLQVLC LIAMEKPVSL KPEHIRDEKV 

       310        320        330        340        350        360 
KVLESVLPIR DDEVVLGQYE GYTDDPTVPD DSNTPTFATT ILRIHNERWE GVPFIVKAGK 

       370        380        390        400        410        420 
ALNSRKAEIR VQFKDVPGDI FRSKKQGRNE FVIRLQPSEA IYMKLTVKQP GLEMSAVQSE 

       430        440        450        460        470        480 
LDLSYGQRYQ GITIPEAYER LILDTIRGDQ QHFVRRDELK ASWQIFTPLL HKIDRGELKP 

       490        500        510 
VPYNPGSRGP AEADELLEKA GYVQTPGYIW IPPTL 

« Hide

References

[1]"Stress responses in alfalfa (Medicago sativa L.) XIX. Transcriptional activation of oxidative pentose phosphate pathway genes at the onset of the isoflavonoid phytoalexin response."
Fahrendorf T., Ni W., Shorrosh B.S., Dixon R.A.
Plant Mol. Biol. 28:885-900(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Apollo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U18238 mRNA. Translation: AAB41552.1.
PIRS57785.

3D structure databases

ProteinModelPortalQ42919.
SMRQ42919. Positions 28-511.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ42919.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00115; UER00408.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
PROSITEPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameG6PD_MEDSA
AccessionPrimary (citable) accession number: Q42919
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: February 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways