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Protein

Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform

Gene
N/A
Organism
Medicago sativa (Alfalfa)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis (By similarity).By similarity

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei71 – 711NADPBy similarity
Binding sitei182 – 1821NADP; via carbonyl oxygenBy similarity
Binding sitei182 – 1821SubstrateBy similarity
Binding sitei250 – 2501SubstrateBy similarity
Binding sitei269 – 2691SubstrateBy similarity
Active sitei274 – 2741Proton acceptorBy similarity
Binding sitei360 – 3601SubstrateBy similarity
Binding sitei365 – 3651SubstrateBy similarity
Binding sitei396 – 3961SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438NADPBy similarity

GO - Molecular functioni

  1. glucose-6-phosphate dehydrogenase activity Source: UniProtKB-EC
  2. NADP binding Source: InterPro

GO - Biological processi

  1. glucose metabolic process Source: UniProtKB-KW
  2. pentose-phosphate shunt Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoform (EC:1.1.1.49)
Short name:
G6PD
OrganismiMedicago sativa (Alfalfa)
Taxonomic identifieri3879 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeTrifolieaeMedicago

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 515515Glucose-6-phosphate 1-dehydrogenase, cytoplasmic isoformPRO_0000068100Add
BLAST

Proteomic databases

PRIDEiQ42919.

Structurei

3D structure databases

ProteinModelPortaliQ42919.
SMRiQ42919. Positions 28-511.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni212 – 2165Substrate bindingBy similarity

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42919-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGTNEWHVER RDSIGTESPV AREVLETGTL SIVVLGASGD LAKKKTFPAL
60 70 80 90 100
FHLYKQELLP PDEVHIFGYA RSKISDDELR NKLRSYLVPE KGASPKQLDD
110 120 130 140 150
VSKFLQLVKY VSGPYDSEDG FRLLDKEISE HEYLKNSKEG SSRRLFYLAL
160 170 180 190 200
PPSVYPSVCK MIKTCCMNKS DLGGWTRVVV EKPFGRDLES AEELSTQIGE
210 220 230 240 250
LFEEPQIYRI DHYLGKELVQ NMLVLRFANR FFLPLWNHNH IDNVQIVFRE
260 270 280 290 300
DFGTDGRGGY FDQYGIIRDI IPNHLLQVLC LIAMEKPVSL KPEHIRDEKV
310 320 330 340 350
KVLESVLPIR DDEVVLGQYE GYTDDPTVPD DSNTPTFATT ILRIHNERWE
360 370 380 390 400
GVPFIVKAGK ALNSRKAEIR VQFKDVPGDI FRSKKQGRNE FVIRLQPSEA
410 420 430 440 450
IYMKLTVKQP GLEMSAVQSE LDLSYGQRYQ GITIPEAYER LILDTIRGDQ
460 470 480 490 500
QHFVRRDELK ASWQIFTPLL HKIDRGELKP VPYNPGSRGP AEADELLEKA
510
GYVQTPGYIW IPPTL
Length:515
Mass (Da):58,923
Last modified:November 1, 1996 - v1
Checksum:i836C1B96F2709FC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18238 mRNA. Translation: AAB41552.1.
PIRiS57785.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18238 mRNA. Translation: AAB41552.1.
PIRiS57785.

3D structure databases

ProteinModelPortaliQ42919.
SMRiQ42919. Positions 28-511.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ42919.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Stress responses in alfalfa (Medicago sativa L.) XIX. Transcriptional activation of oxidative pentose phosphate pathway genes at the onset of the isoflavonoid phytoalexin response."
    Fahrendorf T., Ni W., Shorrosh B.S., Dixon R.A.
    Plant Mol. Biol. 28:885-900(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Apollo.

Entry informationi

Entry nameiG6PD_MEDSA
AccessioniPrimary (citable) accession number: Q42919
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: March 4, 2015
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.