ID   GLNA1_LOTJA             Reviewed;         356 AA.
AC   Q42899; O04880;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   16-JUN-2009, entry version 59.
DE   RecName: Full=Glutamine synthetase cytosolic isozyme;
DE            EC=6.3.1.2;
DE   AltName: Full=GS1;
DE   AltName: Full=Glutamate--ammonia ligase;
GN   Name=GLN1;
OS   Lotus japonicus.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Loteae; Lotus.
OX   NCBI_TaxID=34305;
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RC   TISSUE=Root;
RA   Ruiz M.T., Prosser I.M., Clarkson D.T.;
RT   "Cloning of a subunit of the cytosolic glutamine synthetase from Lotus
RT   japonicus.";
RL   Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9323367; DOI=10.1007/s004380050537;
RA   Thykjaer T., Danielsen D., She Q., Stougaard J.;
RT   "Organization and expression of genes in the genomic region
RT   surrounding the glutamine synthetase gene Gln1 from Lotus japonicus.";
RL   Mol. Gen. Genet. 255:628-636(1997).
CC   -!- CATALYTIC ACTIVITY: ATP + L-glutamate + NH(3) = ADP + phosphate +
CC       L-glutamine.
CC   -!- SUBUNIT: Homooctamer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glutamine synthetase family.
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DR   EMBL; X94299; CAA63963.1; -; mRNA.
DR   EMBL; Y12859; CAA73366.1; -; Genomic_DNA.
DR   UniGene; Lja.3240; -.
DR   SMR; Q42899; 4-355.
DR   BRENDA; 6.3.1.2; 977.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004356; F:glutamate-ammonia ligase activity; IEA:EC.
DR   GO; GO:0006542; P:glutamine biosynthetic process; IEA:InterPro.
DR   InterPro; IPR008147; Gln_synt_beta.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat.
DR   InterPro; IPR008146; Gln_synth_cat.
DR   Gene3D; G3DSA:3.30.590.10; ATP-gua_Ptrans; 1.
DR   Pfam; PF00120; Gln-synt_C; 1.
DR   Pfam; PF03951; Gln-synt_N; 1.
DR   ProDom; PD001057; Gln_synt_C; 1.
DR   PROSITE; PS00180; GLNA_1; 1.
DR   PROSITE; PS00181; GLNA_ATP; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Ligase; Nucleotide-binding.
FT   CHAIN         1    356       Glutamine synthetase cytosolic isozyme.
FT                                /FTId=PRO_0000153175.
FT   CONFLICT     35     35       S -> T (in Ref. 2; CAA73366).
FT   CONFLICT    337    337       N -> Y (in Ref. 1; CAA63963).
SQ   SEQUENCE   356 AA;  39147 MW;  F31F8696F645F731 CRC64;
     MSLLSDLINL NLSETTDKII AEYIWIGGSG LDMRSKARTL PGPVSDPSQL PKWNYDGSST
     GQAPGEDSEV ILYPQAIFRD PFRRGSNILV ICDAYTPAGE PIPTNKRHAA AKVFSHPDVV
     AEVPWYGIEQ EYTLLQKEVN WPVGWPIGGF PGPQGPYYCG IGADKAFGRD IVDAHYKACL
     YAGVNISGIN GEVMPGQWEF QVGPSVGISA GDEVWVARYI LERITEIAGV VLSFDPKPIK
     GDWNGAGAHT NYSTKTMRED GGYEVIKKAI DKLGLRHKEH IAAYGEGNER RLTGRHETAD
     INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIADT TILWKP
//