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Q42899

- GLNA1_LOTJA

UniProt

Q42899 - GLNA1_LOTJA

Protein

Glutamine synthetase cytosolic isozyme

Gene

GLN1

Organism
Lotus japonicus (Lotus corniculatus var. japonicus)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. glutamate-ammonia ligase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glutamine biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Ligase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamine synthetase cytosolic isozyme (EC:6.3.1.2)
    Alternative name(s):
    GS1
    Glutamate--ammonia ligase
    Gene namesi
    Name:GLN1
    OrganismiLotus japonicus (Lotus corniculatus var. japonicus)
    Taxonomic identifieri34305 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaeLoteaeLotus

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 356356Glutamine synthetase cytosolic isozymePRO_0000153175Add
    BLAST

    Proteomic databases

    ProMEXiQ42899.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ42899.
    SMRiQ42899. Positions 4-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glutamine synthetase family.Curated

    Family and domain databases

    Gene3Di3.30.590.10. 1 hit.
    InterProiIPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view]
    PfamiPF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF54368. SSF54368. 1 hit.
    PROSITEiPS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q42899-1 [UniParc]FASTAAdd to Basket

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    MSLLSDLINL NLSETTDKII AEYIWIGGSG LDMRSKARTL PGPVSDPSQL    50
    PKWNYDGSST GQAPGEDSEV ILYPQAIFRD PFRRGSNILV ICDAYTPAGE 100
    PIPTNKRHAA AKVFSHPDVV AEVPWYGIEQ EYTLLQKEVN WPVGWPIGGF 150
    PGPQGPYYCG IGADKAFGRD IVDAHYKACL YAGVNISGIN GEVMPGQWEF 200
    QVGPSVGISA GDEVWVARYI LERITEIAGV VLSFDPKPIK GDWNGAGAHT 250
    NYSTKTMRED GGYEVIKKAI DKLGLRHKEH IAAYGEGNER RLTGRHETAD 300
    INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIADT 350
    TILWKP 356
    Length:356
    Mass (Da):39,147
    Last modified:December 1, 2000 - v2
    Checksum:iF31F8696F645F731
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti35 – 351S → T in CAA73366. (PubMed:9323367)Curated
    Sequence conflicti337 – 3371N → Y in CAA63963. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94299 mRNA. Translation: CAA63963.1.
    Y12859 Genomic DNA. Translation: CAA73366.1.
    UniGeneiLja.3240.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X94299 mRNA. Translation: CAA63963.1 .
    Y12859 Genomic DNA. Translation: CAA73366.1 .
    UniGenei Lja.3240.

    3D structure databases

    ProteinModelPortali Q42899.
    SMRi Q42899. Positions 4-355.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    ProMEXi Q42899.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.30.590.10. 1 hit.
    InterProi IPR008147. Gln_synt_beta.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    IPR008146. Gln_synth_cat_dom.
    IPR027303. Gln_synth_gly_rich_site.
    IPR027302. Gln_synth_N_conserv_site.
    [Graphical view ]
    Pfami PF00120. Gln-synt_C. 1 hit.
    PF03951. Gln-synt_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54368. SSF54368. 1 hit.
    PROSITEi PS00180. GLNA_1. 1 hit.
    PS00181. GLNA_ATP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a subunit of the cytosolic glutamine synthetase from Lotus japonicus."
      Ruiz M.T., Prosser I.M., Clarkson D.T.
      Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Tissue: Root.
    2. "Organization and expression of genes in the genomic region surrounding the glutamine synthetase gene Gln1 from Lotus japonicus."
      Thykjaer T., Danielsen D., She Q., Stougaard J.
      Mol. Gen. Genet. 255:628-636(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiGLNA1_LOTJA
    AccessioniPrimary (citable) accession number: Q42899
    Secondary accession number(s): O04880
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3