Reviewed, UniProtKB/Swiss-Prot Q42899 (GLNA1_LOTJA)

Names and origin

Protein names

Recommended name:
    Glutamine synthetase cytosolic isozyme
    EC=6.3.1.2
Alternative name(s):
    GS1
    Glutamate--ammonia ligase

Gene names

Name:

GLN1

Organism

Lotus japonicus

Taxonomic identifier

34305 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Loteae › Lotus

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 356	356	Glutamine synthetase cytosolic isozyme		PRO_0000153175
Experimental info
	Sequence conflict	35	1	S → T in CAA73366. Ref.2
	Sequence conflict	337	1	N → Y in CAA63963. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q42899-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: F31F8696F645F731	FASTA	356	39,147
	10 20 30 40 50 60 MSLLSDLINL NLSETTDKII AEYIWIGGSG LDMRSKARTL PGPVSDPSQL PKWNYDGSST 70 80 90 100 110 120 GQAPGEDSEV ILYPQAIFRD PFRRGSNILV ICDAYTPAGE PIPTNKRHAA AKVFSHPDVV 130 140 150 160 170 180 AEVPWYGIEQ EYTLLQKEVN WPVGWPIGGF PGPQGPYYCG IGADKAFGRD IVDAHYKACL 190 200 210 220 230 240 YAGVNISGIN GEVMPGQWEF QVGPSVGISA GDEVWVARYI LERITEIAGV VLSFDPKPIK 250 260 270 280 290 300 GDWNGAGAHT NYSTKTMRED GGYEVIKKAI DKLGLRHKEH IAAYGEGNER RLTGRHETAD 310 320 330 340 350 INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIADT TILWKP

References

[1]	"Cloning of a subunit of the cytosolic glutamine synthetase from Lotus japonicus." Ruiz M.T., Prosser I.M., Clarkson D.T. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Root.
[2]	"Organization and expression of genes in the genomic region surrounding the glutamine synthetase gene Gln1 from Lotus japonicus." Thykjaer T., Danielsen D., She Q., Stougaard J. Mol. Gen. Genet. 255:628-636(1997) [PubMed: 9323367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X94299 mRNA. Translation: CAA63963.1. Y12859 Genomic DNA. Translation: CAA73366.1.
UniGene	Lja.3240
3D structure databases
SMR	Q42899. Positions 4-355.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.3.1.2. 977.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
ProDom	PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA1_LOTJA

Accession

Primary (citable) accession number: Q42899
Secondary accession number(s): O04880

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	December 1, 2000
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)