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Reviewed, UniProtKB/Swiss-Prot Q42899 (GLNA1_LOTJA)

Last modified June 16, 2009. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Glutamine synthetase cytosolic isozyme
    EC=6.3.1.2
Alternative name(s):
    GS1
    Glutamate--ammonia ligase
Gene names
Name: GLN1
OrganismLotus japonicus
Taxonomic identifier34305 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonscore eudicotyledonsrosidseurosids IFabalesFabaceaePapilionoideaeLoteaeLotus

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Cytoplasm.

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 356356Glutamine synthetase cytosolic isozyme
PRO_0000153175

Experimental info

Sequence conflict351S → T in CAA73366. Ref.2
Sequence conflict3371N → Y in CAA63963. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q42899-1 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: F31F8696F645F731

FASTA35639,147
        10         20         30         40         50         60 
MSLLSDLINL NLSETTDKII AEYIWIGGSG LDMRSKARTL PGPVSDPSQL PKWNYDGSST 

        70         80         90        100        110        120 
GQAPGEDSEV ILYPQAIFRD PFRRGSNILV ICDAYTPAGE PIPTNKRHAA AKVFSHPDVV 

       130        140        150        160        170        180 
AEVPWYGIEQ EYTLLQKEVN WPVGWPIGGF PGPQGPYYCG IGADKAFGRD IVDAHYKACL 

       190        200        210        220        230        240 
YAGVNISGIN GEVMPGQWEF QVGPSVGISA GDEVWVARYI LERITEIAGV VLSFDPKPIK 

       250        260        270        280        290        300 
GDWNGAGAHT NYSTKTMRED GGYEVIKKAI DKLGLRHKEH IAAYGEGNER RLTGRHETAD 

       310        320        330        340        350 
INTFLWGVAN RGASIRVGRD TEKEGKGYFE DRRPASNMDP YVVTSMIADT TILWKP 

« Hide

References

[1]"Cloning of a subunit of the cytosolic glutamine synthetase from Lotus japonicus."
Ruiz M.T., Prosser I.M., Clarkson D.T.
Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Root.
[2]"Organization and expression of genes in the genomic region surrounding the glutamine synthetase gene Gln1 from Lotus japonicus."
Thykjaer T., Danielsen D., She Q., Stougaard J.
Mol. Gen. Genet. 255:628-636(1997) [PubMed: 9323367] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

X94299 mRNA. Translation: CAA63963.1.
Y12859 Genomic DNA. Translation: CAA73366.1.
UniGeneLja.3240

3D structure databases

SMRQ42899. Positions 4-355.
ModBaseSearch...

Enzyme and pathway databases

BRENDA6.3.1.2. 977.

Family and domain databases

InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat.
IPR008146. Gln_synth_cat.
[Graphical view]
Gene3DG3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
ProDomPD001057. Gln_synt_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA1_LOTJA
AccessionPrimary (citable) accession number: Q42899
Secondary accession number(s): O04880
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: December 1, 2000
Last modified: June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents