Reviewed, UniProtKB/Swiss-Prot Q42885 (AROC2_SOLLC)

Names and origin

Protein names

Recommended name:
    Chorismate synthase 2, chloroplastic
    EC=4.2.3.5
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate phospholyase 2

Gene names

Name:

CS2

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	431 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the last common step of the biosynthesis of aromatic amino acids, produced via the shikimic acid pathway.
Catalytic activity	5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate.
Cofactor	Reduced flavin.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Predominantly expressed in flowers and roots and, to a lesser extent, in stems, leaves, and cotyledons.
Sequence similarities	Belongs to the chorismate synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Lyase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	chorismate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 48	48	Chloroplast Potential
	Chain	49 – 431	383	Chorismate synthase 2, chloroplastic		PRO_0000002297

Sequences

	Sequence		Length	Mass (Da)
	Q42885-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: D72D26C0F4F6B003	FASTA	431	46,871
	10 20 30 40 50 60 MASSMLTKQF LGAPFSSFGS GQQPSKLCSS NLRFPTHRSQ PKRLEIQAAG NTFGNYFRVT 70 80 90 100 110 120 TFGESHGGGV GCIIDGCPPR LPLSESDMQV ELDRRRPGQS RITTPRKETD TCKISSGTAD 130 140 150 160 170 180 GLTTGSPIKV EVPNTDQRGN DYSEMSLAYR PSHADATYDF KYGVRSVQGG GRSSARETIG 190 200 210 220 230 240 RVAAGAVAKK ILKLYSGTEI LAYVSQVHNV VLPEDLVDNQ IVTLEQIESN IVRCPNPEYA 250 260 270 280 290 300 EKMIGAIDYV RVRGDSVGGV VTCIVRNVPR GLGTPVFDKL EAELAKACMS LPATKGFEFG 310 320 330 340 350 360 SGFAGTFMTG SEHNDEFFMD EHDQIRTKTN RSGGIQGGIS NGEIINMRVA FKPTSTIARK 370 380 390 400 410 420 QHTVSRDKHE TELIARGRHD PCVVPRAVPM VEAMVALVLV DQLMTQYAQC MLFPVNLTLQ 430 EPLQPSTTKS A

References

[1]	"Differential expression of tomato (Lycopersicon esculentum L.) genes encoding shikimate pathway isoenzymes. II. Chorismate synthase." Goerlach J., Schmid J., Amrheim N. Plant Mol. Biol. 23:707-716(1993) [PubMed: 8251624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. UC82B.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z21791 mRNA. Translation: CAA79854.1.
PIR	S40409.
UniGene	Les.3660
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.2.3.5. 281054.
Family and domain databases
InterPro	IPR000453. Chorismate_synth. IPR020541. Chorismate_synthase_CS. [Graphical view]
PANTHER	PTHR21085. Chorismate_synth. 1 hit.
Pfam	PF01264. Chorismate_synt. 1 hit. [Graphical view]
PIRSF	PIRSF001456. Chorismate_synth. 1 hit.
ProDom	PD002941. Chorismate_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00033. aroC. 1 hit.
PROSITE	PS00787. CHORISMATE_SYNTHASE_1. 1 hit. PS00788. CHORISMATE_SYNTHASE_2. 1 hit. PS00789. CHORISMATE_SYNTHASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROC2_SOLLC

Accession

Primary (citable) accession number: Q42885

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	September 22, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)