Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calcium-transporting ATPase, endoplasmic reticulum-type

Gene

LCA1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment.1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi319Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi320Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi322Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi324Calcium 2By similarity1
Active sitei3664-aspartylphosphate intermediateBy similarity1
Metal bindingi728MagnesiumBy similarity1
Metal bindingi732MagnesiumBy similarity1
Metal bindingi794Calcium 1By similarity1
Metal bindingi797Calcium 1By similarity1
Metal bindingi822Calcium 2By similarity1
Metal bindingi825Calcium 1By similarity1
Metal bindingi826Calcium 1By similarity1
Metal bindingi826Calcium 2By similarity1
Metal bindingi957Calcium 1By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14597.
BRENDAi3.6.3.8. 3101.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase, endoplasmic reticulum-type (EC:3.6.3.8)
Gene namesi
Name:LCA1Imported
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 63CytoplasmicSequence analysisAdd BLAST63
Transmembranei64 – 84Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini85 – 93LumenalSequence analysis9
Transmembranei94 – 114Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini115 – 213CytoplasmicSequence analysisAdd BLAST99
Transmembranei214 – 234Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini235 – 267LumenalSequence analysisAdd BLAST33
Transmembranei268 – 288Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini289 – 312CytoplasmicSequence analysisAdd BLAST24
Transmembranei313 – 333Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini334 – 800LumenalSequence analysisAdd BLAST467
Transmembranei801 – 821Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini822 – 862CytoplasmicSequence analysisAdd BLAST41
Transmembranei863 – 883Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini884 – 944LumenalSequence analysisAdd BLAST61
Transmembranei945 – 965Helical; Name=8Sequence analysisAdd BLAST21
Topological domaini966 – 981CytoplasmicSequence analysisAdd BLAST16
Transmembranei982 – 1002Helical; Name=9Sequence analysisAdd BLAST21
Topological domaini1003 – 1007LumenalSequence analysis5
Transmembranei1008 – 1028Helical; Name=10Sequence analysisAdd BLAST21
Topological domaini1029 – 1048CytoplasmicSequence analysisAdd BLAST20

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464091 – 1048Calcium-transporting ATPase, endoplasmic reticulum-typeAdd BLAST1048

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ42883.

Expressioni

Tissue specificityi

9-fold higher level in roots compared with leaves.1 Publication

Inductioni

3-fold in leaves and 2-fold in roots 1 day after exposure to high sodium chloride.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi4081.Solyc01g096190.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ42883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1038 – 1042Poly-ArgSequence analysis5

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
InParanoidiQ42883.
KOiK01537.
OMAiFCDGRLF.
OrthoDBiEOG093600UE.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKPFPAWS WSVDQCLKEY QVKLEKGLST YEVDKRRERY GLNELEKEKG
60 70 80 90 100
KPLWRLVLEQ FDDTLVKILL GAAFISFVLA YVNQDETGES GFEAYVEPLV
110 120 130 140 150
ILWILVLNAI VGVWQESNAE KALEALKEMQ GESAKVLRDG YLVPDFPAKE
160 170 180 190 200
LVPGDIVELR VGDKVPADMR VATLKSSTLR VEQSSLTGES MPVTKSTDFL
210 220 230 240 250
ATDDCELQAK ENMVFAGTTV VNGSCICIVV NTGMCTEIGK IQRQIHDASM
260 270 280 290 300
EESDTPLKKK LDEFGNRLTF AIGVVCLVVW AINYKYFLSW EVVDDWPSDF
310 320 330 340 350
RFSFEKCAYY FKIAVALAVA AIPEGLPSVI TTCLALGTRK MAQKNAIVRK
360 370 380 390 400
LQSVETLGCT TVICSDKTGT LTTNQMSVSE FFTLGRKTTA CRVFGVEGTT
410 420 430 440 450
YDPKDGGIMN WNCCKMDANL LLMAEICAIC NDAGVFCDGR LFKATGLPTE
460 470 480 490 500
AALKVLVEKM GVPDSKARCK IRDAQIVSSY LIDRNTVKLG CCDWWMKRSK
510 520 530 540 550
RVATLEFDRV RKSMGVIVRE PNGSNRLLVK GAFESLLERS TYVQLADGST
560 570 580 590 600
VPLDESCRQL LLLKQLEMSS KGLRCLGLAY KDDLGELSGY YAATHPAHKK
610 620 630 640 650
LLDPSCYSSI ESDLVFVGVV GLRDPPREEV HRAVNDCRRA GIKIMVITGD
660 670 680 690 700
NKSTAEAVCR EIQLFSNGEN LRGSSFTGKE FMAFSSQQQI EILSQDGGKV
710 720 730 740 750
FSRAEPRHKQ EIVRMLKEMG EIVAMTGDGV NDAPALKLAD IGIAMGITGT
760 770 780 790 800
EVAKEASDMV LADDNFSTIV SAVAEGRSIY NNMKAFIRYM ISSNVGEVIS
810 820 830 840 850
IFLTAVLGIP ECLIPVQLLW VNLVTDGPPA TALGFNPADV DIMQKPPRKN
860 870 880 890 900
TDALINSWVF FRYMVIGSYV GIATVGIFIV WYTQASFLGI NIVSDGHTLV
910 920 930 940 950
ELSQLRNWGE CSTWTNFTVS PFKAGNRLIT FSDPCEYFTV GKVKAMTLSL
960 970 980 990 1000
SVLVAIEMFN SLNALSEDNS LIKMPPWRNP WLLVAMSLSF ALHSVILYVP
1010 1020 1030 1040
FLADIFGIVP LSLYEWLLVI LLSAPVILID EVLKFVGRRR RRTKLKAA
Length:1,048
Mass (Da):116,128
Last modified:November 1, 1996 - v1
Checksum:iD029CEE2A8E1A294
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96324 Genomic DNA. Translation: AAA34138.1.
AF050495 mRNA. Translation: AAD11617.1.
AF050496 mRNA. Translation: AAD11618.1.
PIRiA46284. S27763.
RefSeqiNP_001234073.1. NM_001247144.2.
XP_010323817.1. XM_010325515.1.
XP_010323824.1. XM_010325522.1.
XP_010323830.1. XM_010325528.1.
UniGeneiLes.34.

Genome annotation databases

EnsemblPlantsiSolyc01g096190.2.1; Solyc01g096190.2.1; Solyc01g096190.2.
GeneIDi543554.
GrameneiSolyc01g096190.2.1; Solyc01g096190.2.1; Solyc01g096190.2.
KEGGisly:543554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96324 Genomic DNA. Translation: AAA34138.1.
AF050495 mRNA. Translation: AAD11617.1.
AF050496 mRNA. Translation: AAD11618.1.
PIRiA46284. S27763.
RefSeqiNP_001234073.1. NM_001247144.2.
XP_010323817.1. XM_010325515.1.
XP_010323824.1. XM_010325522.1.
XP_010323830.1. XM_010325528.1.
UniGeneiLes.34.

3D structure databases

ProteinModelPortaliQ42883.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc01g096190.2.1.

Proteomic databases

PaxDbiQ42883.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSolyc01g096190.2.1; Solyc01g096190.2.1; Solyc01g096190.2.
GeneIDi543554.
GrameneiSolyc01g096190.2.1; Solyc01g096190.2.1; Solyc01g096190.2.
KEGGisly:543554.

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
InParanoidiQ42883.
KOiK01537.
OMAiFCDGRLF.
OrthoDBiEOG093600UE.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14597.
BRENDAi3.6.3.8. 3101.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECAP_SOLLC
AccessioniPrimary (citable) accession number: Q42883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: November 1, 1996
Last modified: September 7, 2016
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.