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Protein

Calcium-transporting ATPase, endoplasmic reticulum-type

Gene

LCA1

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to an endomembrane compartment.1 Publication

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi319 – 3191Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi320 – 3201Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi322 – 3221Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi324 – 3241Calcium 2By similarity
Active sitei366 – 36614-aspartylphosphate intermediateBy similarity
Metal bindingi728 – 7281MagnesiumBy similarity
Metal bindingi732 – 7321MagnesiumBy similarity
Metal bindingi794 – 7941Calcium 1By similarity
Metal bindingi797 – 7971Calcium 1By similarity
Metal bindingi822 – 8221Calcium 2By similarity
Metal bindingi825 – 8251Calcium 1By similarity
Metal bindingi826 – 8261Calcium 1By similarity
Metal bindingi826 – 8261Calcium 2By similarity
Metal bindingi957 – 9571Calcium 1By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14597.
BRENDAi3.6.3.8. 3101.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase, endoplasmic reticulum-type (EC:3.6.3.8)
Gene namesi
Name:LCA1Imported
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6363CytoplasmicSequence analysisAdd
BLAST
Transmembranei64 – 8421Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini85 – 939LumenalSequence analysis
Transmembranei94 – 11421Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini115 – 21399CytoplasmicSequence analysisAdd
BLAST
Transmembranei214 – 23421Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini235 – 26733LumenalSequence analysisAdd
BLAST
Transmembranei268 – 28821Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini289 – 31224CytoplasmicSequence analysisAdd
BLAST
Transmembranei313 – 33321Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini334 – 800467LumenalSequence analysisAdd
BLAST
Transmembranei801 – 82121Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini822 – 86241CytoplasmicSequence analysisAdd
BLAST
Transmembranei863 – 88321Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini884 – 94461LumenalSequence analysisAdd
BLAST
Transmembranei945 – 96521Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini966 – 98116CytoplasmicSequence analysisAdd
BLAST
Transmembranei982 – 100221Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini1003 – 10075LumenalSequence analysis
Transmembranei1008 – 102821Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini1029 – 104820CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10481048Calcium-transporting ATPase, endoplasmic reticulum-typePRO_0000046409Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ42883.

Expressioni

Tissue specificityi

9-fold higher level in roots compared with leaves.1 Publication

Inductioni

3-fold in leaves and 2-fold in roots 1 day after exposure to high sodium chloride.1 Publication

Interactioni

Protein-protein interaction databases

STRINGi4081.Solyc01g096190.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ42883.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1038 – 10425Poly-ArgSequence analysis

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
InParanoidiQ42883.
KOiK01537.
OMAiPIFMDDC.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42883-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKPFPAWS WSVDQCLKEY QVKLEKGLST YEVDKRRERY GLNELEKEKG
60 70 80 90 100
KPLWRLVLEQ FDDTLVKILL GAAFISFVLA YVNQDETGES GFEAYVEPLV
110 120 130 140 150
ILWILVLNAI VGVWQESNAE KALEALKEMQ GESAKVLRDG YLVPDFPAKE
160 170 180 190 200
LVPGDIVELR VGDKVPADMR VATLKSSTLR VEQSSLTGES MPVTKSTDFL
210 220 230 240 250
ATDDCELQAK ENMVFAGTTV VNGSCICIVV NTGMCTEIGK IQRQIHDASM
260 270 280 290 300
EESDTPLKKK LDEFGNRLTF AIGVVCLVVW AINYKYFLSW EVVDDWPSDF
310 320 330 340 350
RFSFEKCAYY FKIAVALAVA AIPEGLPSVI TTCLALGTRK MAQKNAIVRK
360 370 380 390 400
LQSVETLGCT TVICSDKTGT LTTNQMSVSE FFTLGRKTTA CRVFGVEGTT
410 420 430 440 450
YDPKDGGIMN WNCCKMDANL LLMAEICAIC NDAGVFCDGR LFKATGLPTE
460 470 480 490 500
AALKVLVEKM GVPDSKARCK IRDAQIVSSY LIDRNTVKLG CCDWWMKRSK
510 520 530 540 550
RVATLEFDRV RKSMGVIVRE PNGSNRLLVK GAFESLLERS TYVQLADGST
560 570 580 590 600
VPLDESCRQL LLLKQLEMSS KGLRCLGLAY KDDLGELSGY YAATHPAHKK
610 620 630 640 650
LLDPSCYSSI ESDLVFVGVV GLRDPPREEV HRAVNDCRRA GIKIMVITGD
660 670 680 690 700
NKSTAEAVCR EIQLFSNGEN LRGSSFTGKE FMAFSSQQQI EILSQDGGKV
710 720 730 740 750
FSRAEPRHKQ EIVRMLKEMG EIVAMTGDGV NDAPALKLAD IGIAMGITGT
760 770 780 790 800
EVAKEASDMV LADDNFSTIV SAVAEGRSIY NNMKAFIRYM ISSNVGEVIS
810 820 830 840 850
IFLTAVLGIP ECLIPVQLLW VNLVTDGPPA TALGFNPADV DIMQKPPRKN
860 870 880 890 900
TDALINSWVF FRYMVIGSYV GIATVGIFIV WYTQASFLGI NIVSDGHTLV
910 920 930 940 950
ELSQLRNWGE CSTWTNFTVS PFKAGNRLIT FSDPCEYFTV GKVKAMTLSL
960 970 980 990 1000
SVLVAIEMFN SLNALSEDNS LIKMPPWRNP WLLVAMSLSF ALHSVILYVP
1010 1020 1030 1040
FLADIFGIVP LSLYEWLLVI LLSAPVILID EVLKFVGRRR RRTKLKAA
Length:1,048
Mass (Da):116,128
Last modified:November 1, 1996 - v1
Checksum:iD029CEE2A8E1A294
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96324 Genomic DNA. Translation: AAA34138.1.
AF050495 mRNA. Translation: AAD11617.1.
AF050496 mRNA. Translation: AAD11618.1.
PIRiA46284. S27763.
RefSeqiNP_001234073.1. NM_001247144.2.
XP_010323817.1. XM_010325515.1.
XP_010323824.1. XM_010325522.1.
XP_010323830.1. XM_010325528.1.
UniGeneiLes.34.

Genome annotation databases

EnsemblPlantsiSolyc01g096190.2.1; Solyc01g096190.2.1; Solyc01g096190.2.
GeneIDi543554.
GrameneiSolyc01g096190.2.1; Solyc01g096190.2.1; Solyc01g096190.2.
KEGGisly:543554.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96324 Genomic DNA. Translation: AAA34138.1.
AF050495 mRNA. Translation: AAD11617.1.
AF050496 mRNA. Translation: AAD11618.1.
PIRiA46284. S27763.
RefSeqiNP_001234073.1. NM_001247144.2.
XP_010323817.1. XM_010325515.1.
XP_010323824.1. XM_010325522.1.
XP_010323830.1. XM_010325528.1.
UniGeneiLes.34.

3D structure databases

ProteinModelPortaliQ42883.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc01g096190.2.1.

Proteomic databases

PaxDbiQ42883.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSolyc01g096190.2.1; Solyc01g096190.2.1; Solyc01g096190.2.
GeneIDi543554.
GrameneiSolyc01g096190.2.1; Solyc01g096190.2.1; Solyc01g096190.2.
KEGGisly:543554.

Phylogenomic databases

eggNOGiKOG0202. Eukaryota.
COG0474. LUCA.
InParanoidiQ42883.
KOiK01537.
OMAiPIFMDDC.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14597.
BRENDAi3.6.3.8. 3101.

Family and domain databases

Gene3Di1.20.1110.10. 3 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Higher plant Ca2+-ATPase: primary structure and regulation of mRNA abundance by salt."
    Wimmers L.E., Ewing N.N., Bennett A.B.
    Proc. Natl. Acad. Sci. U.S.A. 89:9205-9209(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, INDUCTION.
  2. "Alternative transcription initiation sites generate two LCA1 Ca2+-ATPase mRNA transcripts in tomato roots."
    Navarro-Avino J.P., Hentzen A.E., Bennett A.B.
    Plant Mol. Biol. 40:133-140(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Ohio state 41 Publication.
    Tissue: Root1 Publication.

Entry informationi

Entry nameiECAP_SOLLC
AccessioniPrimary (citable) accession number: Q42883
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.