Reviewed, UniProtKB/Swiss-Prot Q42881 (1A13_SOLLC)

Names and origin

Protein names

Recommended name:
    1-aminocyclopropane-1-carboxylate synthase 3
      Short name=ACC synthase 3
    EC=4.4.1.14
Alternative name(s):
    Le-ACS3
      Short name=ACS-3
    S-adenosyl-L-methionine methylthioadenosine-lyase 3

Gene names

Name:

ACS3

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the formation of 1-aminocyclopropane-1-carboxylate, a direct precursor of ethylene in higher plants.
Catalytic activity	S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.
Cofactor	Pyridoxal phosphate.
Pathway	Alkene biosynthesis; ethylene biosynthesis via S-adenosyl-L-methionine; ethylene from S-adenosyl-L-methionine: step 1/2.
Subunit structure	Homodimer By similarity.
Induction	By flooding.
Sequence similarities	Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.

Ontologies

Keywords
Biological process	Ethylene biosynthesis Fruit ripening
Ligand	Pyridoxal phosphate S-adenosyl-L-methionine
Molecular function	Lyase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	ethylene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW ripening Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-aminocyclopropane-1-carboxylate synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity, transferring nitrogenous groups Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	1-aminocyclopropane-1-carboxylate synthase 3		PRO_0000123913
Amino acid modifications
	Modified residue	272	1	N6-(pyridoxal phosphate)lysine
Experimental info
	Sequence conflict	243	1	F → L in AAB48945. Ref.2
	Sequence conflict	335	1	K → R in AAB48945. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q42881-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: FA5ADCCE3F54F5BC	FASTA	469	53,094
	10 20 30 40 50 60 MKLLSEKATC NSHGQDSSYF LGWQEYEKNP YDEIQNPKGI IQMGLAENQL SFDLLESWLA 70 80 90 100 110 120 QNPDAAGFKR NGESIFRELA LFQDYHGLPA FKNAMTKFMS EIRGNRVSFD SNNLVLTAGA 130 140 150 160 170 180 TSANETLMFC LANQGDAFLL PTPYYPGFDR DLKWRTGAEI VPIHCSSSNG FRITESALEE 190 200 210 220 230 240 AYLDAKKRNL KVKGVLVTNP SNPLGTTLNR NELELLLTFI DEKGIHLISD EIYSGTVFNS 250 260 270 280 290 300 PGFVSVMEVL IEKNYMKTRV WERVHIVYSL SKDLGLPGFR IGAIYSNDEM VVSAATKMSS 310 320 330 340 350 360 FGLVSSQTQY LLSCMLSDKK FTKKYISENQ KRLKKRHAML VKGLKSAGIN CLESNAGLFC 370 380 390 400 410 420 WVDMRHLLSS NNFDAEMDLW KKIVYDVGLN ISPGSSCHCT EPGWFRVCFA NMSEDTLDLA 430 440 450 460 MRRIKDFVES TAPNATNHQN QQQSNANSKK KSFSKWVFRL SFNDRQRER

References

[1]	"Analysis of LE-ACS3, a 1-aminocyclopropane-1-carboxylic acid synthase gene expressed during flooding in the roots of tomato plants." Olson D.C., Oetiker J.H., Yang S.F. J. Biol. Chem. 270:14056-14061(1995) [PubMed: 7775465] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. VFN8. Tissue: Leaf and Root.
[2]	"The tomato 1-aminocyclopropane-1-carboxylate (ACC) synthase multigene family is encoded by at least eight members." Kawakita K., Hennig L., Rottmann W.R., Yu G.X., Zarembinski T.I., Taylor L.D., Theologis A. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Rutgers.
[3]	"Characterization and sequencing of the active site of 1-aminocyclopropane-1-carboxylate synthase." Yip W.K., Dong J.G., Kenny J.W., Thompson G.A., Yang S.F. Proc. Natl. Acad. Sci. U.S.A. 87:7930-7934(1990) [PubMed: 2122449] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L34171 Genomic DNA. Translation: AAA78789.1. U18055 Genomic DNA. Translation: AAB48946.1. U17972 mRNA. Translation: AAB48945.1.
PIR	A57540.
UniGene	Les.3642
3D structure databases
HSSP	HSSP built from PDB template 1M7Y based on UniProtKB P37821.
SMR	Q42881. Positions 12-428.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.4.1.14. 281054.
Family and domain databases
InterPro	IPR001176. ACC_synthase. IPR004839. Aminotransferase_I/II. IPR004838. NHTrfase_class1_PyrdxlP-BS. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
Pfam	PF00155. Aminotran_1_2. 1 hit. [Graphical view]
PRINTS	PR00753. ACCSYNTHASE.
PROSITE	PS00105. AA_TRANSFER_CLASS_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

1A13_SOLLC

Accession

Primary (citable) accession number: Q42881
Secondary accession number(s): Q96571

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	November 1, 1996
Last modified:	September 22, 2009
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)