Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

1-aminocyclopropane-1-carboxylate synthase 3

Gene

ACS3

Organism
Solanum lycopersicum (Tomato) (Lycopersicon esculentum)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 1-aminocyclopropane-1-carboxylate, a direct precursor of ethylene in higher plants.

Catalytic activityi

S-adenosyl-L-methionine = 1-aminocyclopropane-1-carboxylate + methylthioadenosine.

Cofactori

Pathwayi: ethylene biosynthesis via S-adenosyl-L-methionine

This protein is involved in step 1 of the subpathway that synthesizes ethylene from S-adenosyl-L-methionine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 1-aminocyclopropane-1-carboxylate synthase 2 (ACS2), 1-aminocyclopropane-1-carboxylate synthase 3 (ACS3), 1-aminocyclopropane-1-carboxylate synthase 4 (ACS4)
  2. 1-aminocyclopropane-1-carboxylate oxidase 1 (ACO1), 1-aminocyclopropane-1-carboxylate oxidase 2 (ACO2), 1-aminocyclopropane-1-carboxylate oxidase 4 (ACO4)
This subpathway is part of the pathway ethylene biosynthesis via S-adenosyl-L-methionine, which is itself part of Alkene biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes ethylene from S-adenosyl-L-methionine, the pathway ethylene biosynthesis via S-adenosyl-L-methionine and in Alkene biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Ethylene biosynthesis, Fruit ripening

Keywords - Ligandi

Pyridoxal phosphate, S-adenosyl-L-methionine

Enzyme and pathway databases

BRENDAi4.4.1.14. 3101.
UniPathwayiUPA00384; UER00562.

Names & Taxonomyi

Protein namesi
Recommended name:
1-aminocyclopropane-1-carboxylate synthase 3 (EC:4.4.1.14)
Short name:
ACC synthase 3
Alternative name(s):
Le-ACS3
Short name:
ACS-3
S-adenosyl-L-methionine methylthioadenosine-lyase 3
Gene namesi
Name:ACS3
OrganismiSolanum lycopersicum (Tomato) (Lycopersicon esculentum)
Taxonomic identifieri4081 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeSolanoideaeSolaneaeSolanumLycopersicon
Proteomesi
  • UP000004994 Componenti: Chromosome 2

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4694691-aminocyclopropane-1-carboxylate synthase 3PRO_0000123913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei272 – 2721N6-(pyridoxal phosphate)lysine

Proteomic databases

PaxDbiQ42881.

Expressioni

Inductioni

By flooding.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi4081.Solyc02g091990.2.1.

Structurei

3D structure databases

ProteinModelPortaliQ42881.
SMRiQ42881. Positions 12-428.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0256. Eukaryota.
COG0436. LUCA.
InParanoidiQ42881.
KOiK01762.
OMAiSHHQMLK.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42881-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLLSEKATC NSHGQDSSYF LGWQEYEKNP YDEIQNPKGI IQMGLAENQL
60 70 80 90 100
SFDLLESWLA QNPDAAGFKR NGESIFRELA LFQDYHGLPA FKNAMTKFMS
110 120 130 140 150
EIRGNRVSFD SNNLVLTAGA TSANETLMFC LANQGDAFLL PTPYYPGFDR
160 170 180 190 200
DLKWRTGAEI VPIHCSSSNG FRITESALEE AYLDAKKRNL KVKGVLVTNP
210 220 230 240 250
SNPLGTTLNR NELELLLTFI DEKGIHLISD EIYSGTVFNS PGFVSVMEVL
260 270 280 290 300
IEKNYMKTRV WERVHIVYSL SKDLGLPGFR IGAIYSNDEM VVSAATKMSS
310 320 330 340 350
FGLVSSQTQY LLSCMLSDKK FTKKYISENQ KRLKKRHAML VKGLKSAGIN
360 370 380 390 400
CLESNAGLFC WVDMRHLLSS NNFDAEMDLW KKIVYDVGLN ISPGSSCHCT
410 420 430 440 450
EPGWFRVCFA NMSEDTLDLA MRRIKDFVES TAPNATNHQN QQQSNANSKK
460
KSFSKWVFRL SFNDRQRER
Length:469
Mass (Da):53,094
Last modified:November 1, 1996 - v1
Checksum:iFA5ADCCE3F54F5BC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti243 – 2431F → L in AAB48945 (Ref. 2) Curated
Sequence conflicti335 – 3351K → R in AAB48945 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34171 Genomic DNA. Translation: AAA78789.1.
U18055 Genomic DNA. Translation: AAB48946.1.
U17972 mRNA. Translation: AAB48945.1.
PIRiA57540.
RefSeqiNP_001234026.2. NM_001247097.2.
UniGeneiLes.19285.
Les.3642.

Genome annotation databases

EnsemblPlantsiSolyc02g091990.2.1; Solyc02g091990.2.1; Solyc02g091990.2.
GeneIDi778292.
GrameneiSolyc02g091990.2.1; Solyc02g091990.2.1; Solyc02g091990.2.
KEGGisly:778292.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L34171 Genomic DNA. Translation: AAA78789.1.
U18055 Genomic DNA. Translation: AAB48946.1.
U17972 mRNA. Translation: AAB48945.1.
PIRiA57540.
RefSeqiNP_001234026.2. NM_001247097.2.
UniGeneiLes.19285.
Les.3642.

3D structure databases

ProteinModelPortaliQ42881.
SMRiQ42881. Positions 12-428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4081.Solyc02g091990.2.1.

Proteomic databases

PaxDbiQ42881.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiSolyc02g091990.2.1; Solyc02g091990.2.1; Solyc02g091990.2.
GeneIDi778292.
GrameneiSolyc02g091990.2.1; Solyc02g091990.2.1; Solyc02g091990.2.
KEGGisly:778292.

Phylogenomic databases

eggNOGiKOG0256. Eukaryota.
COG0436. LUCA.
InParanoidiQ42881.
KOiK01762.
OMAiSHHQMLK.

Enzyme and pathway databases

UniPathwayiUPA00384; UER00562.
BRENDAi4.4.1.14. 3101.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR004839. Aminotransferase_I/II.
IPR004838. NHTrfase_class1_PyrdxlP-BS.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00105. AA_TRANSFER_CLASS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Analysis of LE-ACS3, a 1-aminocyclopropane-1-carboxylic acid synthase gene expressed during flooding in the roots of tomato plants."
    Olson D.C., Oetiker J.H., Yang S.F.
    J. Biol. Chem. 270:14056-14061(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. VFN8.
    Tissue: Leaf and Root.
  2. "The tomato 1-aminocyclopropane-1-carboxylate (ACC) synthase multigene family is encoded by at least eight members."
    Kawakita K., Hennig L., Rottmann W.R., Yu G.X., Zarembinski T.I., Taylor L.D., Theologis A.
    Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Rutgers.
  3. "Characterization and sequencing of the active site of 1-aminocyclopropane-1-carboxylate synthase."
    Yip W.K., Dong J.G., Kenny J.W., Thompson G.A., Yang S.F.
    Proc. Natl. Acad. Sci. U.S.A. 87:7930-7934(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry namei1A13_SOLLC
AccessioniPrimary (citable) accession number: Q42881
Secondary accession number(s): Q96571
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 1, 1996
Last modified: June 8, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.