Q42876 (AMPL2_SOLLC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leucine aminopeptidase 2, chloroplastic
EC=3.4.11.1

Alternative name(s):
Leucyl aminopeptidase 2
Short name=LAP 2
Proline aminopeptidase 2
EC=3.4.11.5
Prolyl aminopeptidase 2

Gene names

Name:

LAPA2

Organism

Solanum lycopersicum (Tomato) (Lycopersicon esculentum)

Taxonomic identifier

4081 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum › Lycopersicon

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins.
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. Release of N-terminal proline from a peptide.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Plastid › chloroplast.
Induction	By wounding.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: InterPro metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 48	48	Chloroplast Potential
	Chain	49 – 569	521	Leucine aminopeptidase 2, chloroplastic		PRO_0000026804
Regions
	Compositional bias	167 – 172	6	Poly-Ala
Sites
	Active site	351	1	Potential
	Active site	428	1	Potential
	Metal binding	339	1	Zinc 2 By similarity
	Metal binding	344	1	Zinc 1 By similarity
	Metal binding	344	1	Zinc 2 By similarity
	Metal binding	364	1	Zinc 2 By similarity
	Metal binding	424	1	Zinc 1 By similarity
	Metal binding	426	1	Zinc 1 By similarity
	Metal binding	426	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42876 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: E8DADAC26A3DC47B	FASTA	569	59,549
	10 20 30 40 50 60 MNGVLCSSSS SFHSYPSIFT KFQSSPIWSF SISVTPLCSR RAKRMAHSIA RDTLGLTHTN 70 80 90 100 110 120 QSDAPKISFA AKEIDLVEWK GDILTVGATE KDLARDGNSK FQNPLLQKLD SKLSGLLSEA 130 140 150 160 170 180 SSEEDFSGKA GQSTILRLPG LGSKRIALVG LGSPTSSTAA YRCLGEAAAA AAKSAQASNI 190 200 210 220 230 240 AIALASTDGL SAELKLSSAS AITTGAVLGT FEDNRFKSES KKPTLKSLDI LGLGTGPEIE 250 260 270 280 290 300 KKIKYAADVC AGVILGRELV NAPANVLTPA VLAEEAKKIA STYSDVFSAN ILDVEQCKEL 310 320 330 340 350 360 KMGSYLRVAA ASANPAHFIH LCYKPSSGEI KKKIALVGKG LTFDSGGYNI KTGPGCSIEL 370 380 390 400 410 420 MKFDMGGAAA VLGAAKALGQ IKPAGVEVHF IVAACENMIS GTGMRPGDII TASNGKTIEV 430 440 450 460 470 480 NNTDAEGRLT LSVGISCNQG VEKIVDLATL TGACVVALGP SIAGIFTPSD DLAKEVVAAS 490 500 510 520 530 540 EVSGEKLWRL PMEDSYWDSM KSGVADMVNT GGRPGGAITA ALFLKQFVNE KVQWMHIDLA 550 560 GPVWSDKKKN ATGFGVSTLV EWVLKNSTN

References

[1]	"Nature and regulation of pistil-expressed genes in tomato." Milligan S.B., Gasser C.S. Plant Mol. Biol. 28:691-711(1995) [PubMed: 7647301] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. VF36. Tissue: Pistil.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U20594 mRNA. Translation: AAA80499.1.
PIR	S57812.
RefSeq	NP_001233884.1. NM_001246955.1.
UniGene	Les.307.
3D structure databases
ProteinModelPortal	Q42876.
ModBase	Search...
Protein family/group databases
MEROPS	M17.002.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	544277.
Family and domain databases
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR023042. Peptidase_M17_cytosol_amino. IPR008283. Peptidase_M17_N. [Graphical view]
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPL2_SOLLC

Accession

Primary (citable) accession number: Q42876

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program