Q42870 (PLSC_LIMDO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-acyl-sn-glycerol-3-phosphate acyltransferase
Short name=1-AGP acyltransferase
Short name=1-AGPAT
EC=2.3.1.51

Alternative name(s):
Lysophosphatidic acid acyltransferase
Short name=LPAAT

Gene names

Name:

PLSC

Organism

Limnanthes douglasii (Douglas's meadowfoam)

Taxonomic identifier

28973 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Limnanthaceae › Limnanthes

Protein attributes

Sequence length	281 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position. This enzyme uses erucoyl-CoA as an acyl donor.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 2/3.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 281	281	1-acyl-sn-glycerol-3-phosphate acyltransferase		PRO_0000208187
Regions
	Transmembrane	40 – 60	21	Helical; Potential
	Transmembrane	71 – 91	21	Helical; Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Motif	109 – 114	6	HXXXXD motif
Experimental info
	Sequence conflict	46	1	I → V in CAA86877. Ref.2
	Sequence conflict	188	1	R → G in CAA86877. Ref.2
	Sequence conflict	262	1	V → I in CAA86877. Ref.2
	Sequence conflict	281	1	N → K in CAA86877. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q42870 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 9C880BD9E492EE2A	FASTA	281	31,717
	10 20 30 40 50 60 MAKTRTSSLR NRRQLKPAVA ATADDDKDGV FMVLLSCFKI FVCFAIVLIT AVAWGLIMVL 70 80 90 100 110 120 LLPWPYMRIR LGNLYGHIIG GLVIWIYGIP IKIQGSEHTK KRAIYISNHA SPIDAFFVMW 130 140 150 160 170 180 LAPIGTVGVA KKEVIWYPLL GQLYTLAHHI RIDRSNPAAA IQSMKEAVRV ITEKNLSLIM 190 200 210 220 230 240 FPEGTRSRDG RLLPFKKGFV HLALQSHLPI VPMILTGTHL AWRKGTFRVR PVPITVKYLP 250 260 270 280 PINTDDWTVD KIDDYVKMIH DVYVRNLPAS QKPLGSTNRS N

References

[1]	"A plant acyltransferase involved in triacylglycerol biosynthesis complements an Escherichia coli sn-1-acylglycerol-3-phosphate acyltransferase mutant." Hanke C., Wolter F.P., Coleman J., Peterek G., Frentzen M. Eur. J. Biochem. 232:806-810(1995) [PubMed: 7588719] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification of a cDNA that encodes a 1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthes douglasii." Brown A.P., Brough C.L., Kroon J., Slabas A.R. Plant Mol. Biol. 29:267-278(1995) [PubMed: 7579178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X83266 mRNA. Translation: CAA58239.1. Z46836 mRNA. Translation: CAA86877.1.
PIR	S60477.
3D structure databases
ProteinModelPortal	Q42870.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.51. 3028.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR004552. AGP_acyltrans. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00530. AGP_acyltrn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSC_LIMDO

Accession

Primary (citable) accession number: Q42870
Secondary accession number(s): Q40120

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	May 31, 2011
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program