Reviewed, UniProtKB/Swiss-Prot Q42858 (PAL2_IPOBA)

Names and origin

Protein names	Recommended name:     Phenylalanine ammonia-lyase     EC=4.3.1.24
Organism	Ipomoea batatas (Sweet potato) (Batate)
Taxonomic identifier	4120 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Convolvulaceae › Ipomoeeae › Ipomoea

Protein attributes

Sequence length	708 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a key enzyme of plant metabolism catalyzing the first reaction in the biosynthesis from L-phenylalanine of a wide variety of natural products based on the phenylpropane skeleton.
Catalytic activity	L-phenylalanine = trans-cinnamate + ammonia.
Pathway	Phenylpropanoid metabolism; cinnamic acid biosynthesis; trans-cinnamic acid from L-phenylalanine: step 1/1.
Subcellular location	Cytoplasm Probable.
Post-translational modification	Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly By similarity.
Sequence similarities	Belongs to the PAL/histidase family.

Ontologies

Keywords
Biological process	Phenylpropanoid metabolism
Cellular component	Cytoplasm
Molecular function	Lyase
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: InterPro biosynthetic process Inferred from electronic annotation. Source: InterPro phenylpropanoid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ammonia ligase activity Inferred from electronic annotation. Source: InterPro ammonia-lyase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 708	708	Phenylalanine ammonia-lyase		PRO_0000215394
Amino acid modifications
	Modified residue	195	1	2,3-didehydroalanine (Ser) By similarity
	Cross-link	194 ↔ 196		5-imidazolinone (Ala-Gly) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42858-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: C10F736C41F1BEC6	FASTA	708	77,383
	10 20 30 40 50 60 MEGAIANGHT NDFCIKVDPL NWEMAADSLK GSHLDEVKRM VAEFRNPAVK IGGQTLTSLR 70 80 90 100 110 120 SPPIAARDNA SKWSSPRLPA RRESSSDWVM NSMNNGTDSY GVTTGFGATS HRRTKNGHAL 130 140 150 160 170 180 QQELIRFLNA GIFGTGTGAS HTLPHSATRA AMLVRINTLL QGYSGIRFEI LEAITKLLNH 190 200 210 220 230 240 NITPCLPLRG TITASGDLVP LSYIAGLLTG RPNSKAVGPN GEALTAEEAF KLAGVQGGFF 250 260 270 280 290 300 ELQPKEGLAL VNGTAVGSGM ASMVLFEANV LAVLSEVLSA IFAEVMNGKP EFTDHLTHKL 310 320 330 340 350 360 KHHPGQIEAA AIMEHILDRS YYMKAAQKLH EMDPLQKPKQ DRYALRTSPQ WLGPQIEVIR 370 380 390 400 410 420 QATKMIEREI NSVNDNPLID VSRNKALHGG NFQGTPIGVS MDNSRLALAS IGKLIFAQFS 430 440 450 460 470 480 ELVNDYYNNG LPSNLTAGRN PSLDYGFKGV EIAMASYCSE LQFLANPVTN HVQSAEQHNQ 490 500 510 520 530 540 DVNSLGLISA RKTAEAVDVL KLMSSTYLVA LCQAIDLRHL EENLKNAVRN TVNQVAKRTL 550 560 570 580 590 600 TMGVNGELHP SRFCEKDLLR VVDREYVFAY ADDPCSANYP LFQKLRQVLV DHALQNGEHE 610 620 630 640 650 660 KNVSTSIFQK IAAFEDELKA VLPKEVEGAR SAIENGNPAI PNRITECRSY PLYKFVREEL 670 680 690 700 GTEMLTGEKV KSPGEVCDKV FTAVCDGGII DPLLECLKSW DGAPLPIC

References

[1]	Tanaka Y. Submitted (DEC-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Beniazuma. Tissue: Root.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D78640 mRNA. Translation: BAA11459.1.
PIR	T10909.
3D structure databases
HSSP	HSSP built from PDB template 1GKM based on UniProtKB P21310.
SMR	Q42858. Positions 18-708.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.3.1.5. 21210.
Family and domain databases
InterPro	IPR001106. Phe/His_NH3-lyase. IPR005922. Phe_NH3-lyase. [Graphical view]
Pfam	PF00221. PAL. 1 hit. [Graphical view]
TIGRFAMs	TIGR01226. phe_am_lyase. 1 hit.
PROSITE	PS00488. PAL_HISTIDASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PAL2_IPOBA

Accession

Primary (citable) accession number: Q42858

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)