ID   PORB_HORVU              Reviewed;         395 AA.
AC   Q42850;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=Protochlorophyllide reductase B, chloroplastic;
DE            Short=PCR B;
DE            EC=1.3.1.33;
DE   AltName: Full=NADPH-protochlorophyllide oxidoreductase B;
DE            Short=POR B;
DE   Flags: Precursor;
GN   Name=PORB;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Pooideae; Triticeae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=95241482; PubMed=7724548; DOI=10.1073/pnas.92.8.3254;
RA   Holtorf H., Reinbothe S., Reinbothe C., Bereza B., Apel K.;
RT   "Two routes of chlorophyllide synthesis that are differentially
RT   regulated by light in barley (Hordeum vulgare L.).";
RL   Proc. Natl. Acad. Sci. U.S.A. 92:3254-3258(1995).
CC   -!- FUNCTION: Phototransformation of protochlorophyllide (Pchlide) to
CC       chlorophyllide (Chlide).
CC   -!- CATALYTIC ACTIVITY: Chlorophyllide a + NADP(+) =
CC       protochlorophyllide + NADPH.
CC   -!- PATHWAY: Porphyrin biosynthesis; chlorophyll biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases
CC       (SDR) family. POR subfamily.
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DR   EMBL; X84738; CAA59228.1; -; mRNA.
DR   PIR; S52285; S52285.
DR   UniGene; Hv.601; -.
DR   HSSP; P14061; 1FDS.
DR   Gramene; Q42850; -.
DR   BRENDA; 1.3.1.33; 283.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0016630; F:protochlorophyllide reductase activity; IEA:EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   InterPro; IPR002198; DH_sc/Rdtase_SDR.
DR   InterPro; IPR002347; Glc/ribitol_DH.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR005979; Prochl_reduct.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR19410; ADH_short_C2; 1.
DR   Pfam; PF00106; adh_short; 1.
DR   PRINTS; PR00081; GDHRDH.
DR   TIGRFAMs; TIGR01289; LPOR; 1.
PE   2: Evidence at transcript level;
KW   Chlorophyll biosynthesis; Chloroplast; NADP; Oxidoreductase;
KW   Photosynthesis; Plastid; Transit peptide.
FT   TRANSIT       1     59       Chloroplast (Potential).
FT   CHAIN        60    395       Protochlorophyllide reductase B,
FT                                chloroplastic.
FT                                /FTId=PRO_0000023294.
SQ   SEQUENCE   395 AA;  42148 MW;  BFCB90122D6F64B9 CRC64;
     MALQAATSFL PSALSARKEG AAKDSAFFGV RLADGLKLDA TSLGLRTKRV NTSSVAIRAQ
     AAAVSAPTAT PASPAGKKTV RTGNAIITGA SSGLGLATAK ALAESGKWHV IMACRDYLKT
     ARAARAAGMP KGSYTIVHLD LASLDSVRQF VKNVRQLDMP IDVVVCNAAV YQPTAKEPSF
     TADGFEMSVG VNHLGHFLLA RELLEDLKAS DYPSKRLIIV GSITGNTNTL AGNVPPKANL
     GDLRGLAAGL NGVGSAAMID GAEFDGAKAY KDSKVCNMLT MQEFHRRYHE ETGVTFASLY
     PGCIATTGLF REHIPLFRLL FPPFQKYITK GYVSEEEAGK RLAQVVSEPS LTKSGVYWSW
     NKNSASFENQ LSEEASDTEK ARKVWELSEK LVGLA
//