Reviewed, UniProtKB/Swiss-Prot Q42850 (PORB_HORVU)

Names and origin

Protein names

Recommended name:
    Protochlorophyllide reductase B, chloroplastic
      Short name=PCR B
    EC=1.3.1.33
Alternative name(s):
    NADPH-protochlorophyllide oxidoreductase B
      Short name=POR B

Gene names

Name:

PORB

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	395 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phototransformation of protochlorophyllide (Pchlide) to chlorophyllide (Chlide).
Catalytic activity	Chlorophyllide a + NADP+ = protochlorophyllide + NADPH.
Pathway	Porphyrin biosynthesis; chlorophyll biosynthesis.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. POR subfamily.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Photosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	binding Inferred from electronic annotation. Source: InterPro protochlorophyllide reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 59	59	Chloroplast Potential
	Chain	60 – 395	336	Protochlorophyllide reductase B, chloroplastic		PRO_0000023294

Sequences

	Sequence		Length	Mass (Da)
	Q42850-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BFCB90122D6F64B9	FASTA	395	42,148
	10 20 30 40 50 60 MALQAATSFL PSALSARKEG AAKDSAFFGV RLADGLKLDA TSLGLRTKRV NTSSVAIRAQ 70 80 90 100 110 120 AAAVSAPTAT PASPAGKKTV RTGNAIITGA SSGLGLATAK ALAESGKWHV IMACRDYLKT 130 140 150 160 170 180 ARAARAAGMP KGSYTIVHLD LASLDSVRQF VKNVRQLDMP IDVVVCNAAV YQPTAKEPSF 190 200 210 220 230 240 TADGFEMSVG VNHLGHFLLA RELLEDLKAS DYPSKRLIIV GSITGNTNTL AGNVPPKANL 250 260 270 280 290 300 GDLRGLAAGL NGVGSAAMID GAEFDGAKAY KDSKVCNMLT MQEFHRRYHE ETGVTFASLY 310 320 330 340 350 360 PGCIATTGLF REHIPLFRLL FPPFQKYITK GYVSEEEAGK RLAQVVSEPS LTKSGVYWSW 370 380 390 NKNSASFENQ LSEEASDTEK ARKVWELSEK LVGLA

References

[1]	"Two routes of chlorophyllide synthesis that are differentially regulated by light in barley (Hordeum vulgare L.)." Holtorf H., Reinbothe S., Reinbothe C., Bereza B., Apel K. Proc. Natl. Acad. Sci. U.S.A. 92:3254-3258(1995) [PubMed: 7724548] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X84738 mRNA. Translation: CAA59228.1.
PIR	S52285.
UniGene	Hv.601
3D structure databases
SMR	Q42850. Positions 84-390.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-46279N.
Organism-specific databases
Gramene	Q42850.
Enzyme and pathway databases
BRENDA	1.3.1.33. 283.
Gene expression databases
Genevestigator	Q42850.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR005979. Prochl_reduct. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH.
TIGRFAMs	TIGR01289. LPOR. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PORB_HORVU

Accession

Primary (citable) accession number: Q42850

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)