Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Glutamyl-tRNA reductase 1, chloroplastic

Gene

HEMA1

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).1 Publication

Miscellaneous

Was shown to bind heme, but the precise role of heme is unclear.
During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA (By similarity).By similarity

Catalytic activityi

L-glutamate 1-semialdehyde + NADP+ + tRNA(Glu) = L-glutamyl-tRNA(Glu) + NADPH.

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu).
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Glutamyl-tRNA reductase 2 (HEMA2), Glutamyl-tRNA reductase 1, chloroplastic (HEMA1), Glutamyl-tRNA reductase 3, chloroplastic (HEMA3), Glutamyl-tRNA reductase (hemA1)
  2. Glutamate-1-semialdehyde 2,1-aminomutase, chloroplastic (GSA)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-aminolevulinate from L-glutamyl-tRNA(Glu), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei125NucleophileBy similarity1
Sitei174Important for activityBy similarity1
Binding sitei184SubstrateBy similarity1
Binding sitei195SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi266 – 271NADPBy similarity6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processChlorophyll biosynthesis, Porphyrin biosynthesis
LigandNADP

Enzyme and pathway databases

BRENDAi1.2.1.70 2687
UniPathwayiUPA00251; UER00316

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA reductase 1, chloroplastic (EC:1.2.1.70)
Short name:
GluTR
Gene namesi
Name:HEMA1
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 43Chloroplast1 PublicationAdd BLAST43
ChainiPRO_000001331144 – 527Glutamyl-tRNA reductase 1, chloroplasticAdd BLAST484

Proteomic databases

PRIDEiQ42843

Expressioni

Gene expression databases

ExpressionAtlasiQ42843 baseline and differential

Interactioni

Subunit structurei

Homodimer.1 Publication

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B29model-A76-460[»]
1B61model-A76-460[»]
ProteinModelPortaliQ42843
SMRiQ42843
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni124 – 127Substrate bindingBy similarity4
Regioni189 – 191Substrate bindingBy similarity3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi286 – 289Poly-Val4

Sequence similaritiesi

Belongs to the glutamyl-tRNA reductase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IGX5 Eukaryota
COG0373 LUCA
KOiK02492

Family and domain databases

Gene3Di3.30.460.30, 1 hit
HAMAPiMF_00087 Glu_tRNA_reductase, 1 hit
InterProiView protein in InterPro
IPR000343 4pyrrol_synth_GluRdtase
IPR015896 4pyrrol_synth_GluRdtase_dimer
IPR015895 4pyrrol_synth_GluRdtase_N
IPR018214 GluRdtase_CS
IPR036453 GluRdtase_dimer_dom_sf
IPR036343 GluRdtase_N_sf
IPR036291 NAD(P)-bd_dom_sf
IPR006151 Shikm_DH/Glu-tRNA_Rdtase
PfamiView protein in Pfam
PF00745 GlutR_dimer, 1 hit
PF05201 GlutR_N, 1 hit
PF01488 Shikimate_DH, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
SSF69075 SSF69075, 1 hit
SSF69742 SSF69742, 1 hit
TIGRFAMsiTIGR01035 hemA, 1 hit
PROSITEiView protein in PROSITE
PS00747 GLUTR, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42843-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGATSATAA AGAFAAAKAR GPAAACPWLV AAGGRRRSGV VRCDAGGDAQ
60 70 80 90 100
AASKAASITA LEQFKISADR YMKEKSSIAV IGLSVHTAPV EMREKLAVAE
110 120 130 140 150
ELWPRAISEL TSLNHIEEAA VLSTCNRMEI YVVALSWNRG IREVVDWMSK
160 170 180 190 200
KSGIPASELR EHLFMLRDSD ATRHLFEVSA GLDSLVLGEG QILAQVKQVV
210 220 230 240 250
RNGQNSGGLG KNIDRMFKDA ITAGKRVRCE TNISAGAVSV SSAAVELAMM
260 270 280 290 300
KLPKSECLSA RMLLIGAGKM GKLVVKHLIA KGCKKVVVVN RSVERVDAIR
310 320 330 340 350
EEMKDIEIVY RPLTEMYEAA ADADVVFTST ASESLLFTKE HAEVLPPISL
360 370 380 390 400
AMGGVRLFVD ISVPRNVGAC LSEVEHARVY NVDDLKEVVE ANKEDRVRKA
410 420 430 440 450
MEAQTIITQE LKRFEAWRDS LETVPTIKKL RSYADRIRAS ELEKCLQKIG
460 470 480 490 500
EDNLNKKMRR SIEELSTGIV NKLLHGPLQH LRCDGSDSRT LDETLENMHA
510 520
LNRMFSLDTE KAVLEQKIKA KVEKTQS
Length:527
Mass (Da):57,653
Last modified:November 1, 1997 - v1
Checksum:iD329E56458BE1165
GO

Sequence cautioni

The sequence CAA60054 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92403 mRNA Translation: CAA63140.1
X86101 mRNA Translation: CAA60054.1 Different initiation.
PIRiT05732

Genome annotation databases

KEGGiag:CAA63140

Similar proteinsi

Entry informationi

Entry nameiHEM11_HORVU
AccessioniPrimary (citable) accession number: Q42843
Secondary accession number(s): Q42844
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: May 23, 2018
This is version 121 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health