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Reviewed, UniProtKB/Swiss-Prot Q42840 (HEM6_HORVU)

Last modified January 19, 2010. Version 57. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Coproporphyrinogen-III oxidase, chloroplastic
      Short name=Coproporphyrinogenase
      Short name=Coprogen oxidase
    EC=1.3.3.3
Gene names
Name: CPX
OrganismHordeum vulgare (Barley)
Taxonomic identifier4513 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

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Protein attributes

Sequence length391 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity.

Catalytic activity

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1.

Subunit structure

Homodimer By similarity.

Subcellular location

Plastid › chloroplast Probable.

Sequence similarities

Belongs to the aerobic coproporphyrinogen-III oxidase family.

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Ontologies

Keywords
   Biological processHeme biosynthesis
Porphyrin biosynthesis
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processheme biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncoproporphyrinogen oxidase activity

Inferred from electronic annotation. Source: EC

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 391Coproporphyrinogen-III oxidase, chloroplasticPRO_0000006032

Regions

Region125 – 13410Important for dimerization By similarity
Region195 – 1973Substrate binding By similarity
Region331 – 36636Important for dimerization By similarity
Region349 – 3546Substrate binding By similarity

Sites

Active site1931Proton donor By similarity
Binding site1791Substrate By similarity
Site2661Important for dimerization By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q42840-1 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: BD3549559F2EB2CB

FASTA39143,556
        10         20         30         40         50         60 
MASSLLTTPS QTLAPNPAAA RARRSSPAAA QVSFSSPLLP GRRALRCARP VAIEKEVPEK 

        70         80         90        100        110        120 
EAPTTFLRED GSGAGSGSVR ERFEGMIRRV QGEICAALEE ADGSGKRFVE DVWSRPGGVC 

       130        140        150        160        170        180 
VHSRVLQDGN VFEKAGVNVS AVIGVCPRSA YRAAKGAAKN GAADGHKAGP VPFFSAGISS 

       190        200        210        220        230        240 
VLHPKNPFAP TLHFNYRYFE TDAPKDVPGA PRSWWFGGGT DLTPSYLIEE DVKHFHSVQK 

       250        260        270        280        290        300 
QTCDKFDPSF YPRFKKWCDD YFYIKHRNER RGLGGIFFDD LNDYDQDMLL NFATECAGSV 

       310        320        330        340        350        360 
IPAYIPIIER RKDTPFNEEQ KAWQQVRRGR YVEFNLVYDR GTTFGLKTGG RIESILVSLP 

       370        380        390 
LTARWEYDHK PEEGSEEWKL LDACINPKEW L

« Hide

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References

[1]"Coproporphyrinogen III oxidase from barley and tobacco -- sequence analysis and initial expression studies."
Kruse E., Mock H.-P., Grimm B.
Planta 196:796-803(1995) [PubMed: 7580857] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Svalofs Bonus.
Tissue: Leaf.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X82830 mRNA. Translation: CAA58037.1.
PIRT04486.

3D structure databases

SMRQ42840. Positions 76-391.
ModBaseSearch...

Organism-specific databases

GrameneQ42840.

Enzyme and pathway databases

BRENDA1.3.3.3. 283.

Gene expression databases

GenevestigatorQ42840.

Family and domain databases

InterProIPR001260. Coprogen_oxidas.
IPR018375. Coproporphyrinogen-3_ox_CS.
[Graphical view]
Gene3DG3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
PANTHERPTHR10755. Coprogen_oxidas. 1 hit.
PfamPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSPR00073. COPRGNOXDASE.
PROSITEPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameHEM6_HORVU
AccessionPrimary (citable) accession number: Q42840
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents