ID   HEM2_HORVU              Reviewed;         428 AA.
AC   Q42836;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 55.
DE   RecName: Full=Delta-aminolevulinic acid dehydratase, chloroplastic;
DE            Short=ALADH;
DE            EC=4.2.1.24;
DE   AltName: Full=Porphobilinogen synthase;
DE   Flags: Precursor;
GN   Name=HEMB; Synonyms=ALAD;
OS   Hordeum vulgare (Barley).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Poales; Poaceae; BEP clade;
OC   Pooideae; Triticeae; Hordeum.
OX   NCBI_TaxID=4513;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Bonus;
RA   Bougri O.;
RL   Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: 2 5-aminolevulinate = porphobilinogen + 2
CC       H(2)O.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- PATHWAY: Porphyrin metabolism; protoporphyrin-IX biosynthesis;
CC       coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
CC   -!- SUBUNIT: Homooctamer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the ALADH family.
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DR   EMBL; X92402; CAA63139.1; -; mRNA.
DR   PIR; T04472; T04472.
DR   HSSP; P13716; 1E51.
DR   Gramene; Q42836; -.
DR   BRENDA; 4.2.1.24; 283.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004655; F:porphobilinogen synthase activity; IEA:EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001731; 4pyrrol_synth_porphobiln_synth.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   PANTHER; PTHR11458; AlaD_dehydratase; 1.
DR   Pfam; PF00490; ALAD; 1.
DR   PRINTS; PR00144; DALDHYDRTASE.
DR   ProDom; PD002304; AlaD_dehydratase; 1.
DR   PROSITE; PS00169; D_ALA_DEHYDRATASE; 1.
PE   2: Evidence at transcript level;
KW   Chlorophyll biosynthesis; Chloroplast; Lyase; Magnesium; Plastid;
KW   Porphyrin biosynthesis; Transit peptide.
FT   TRANSIT       1      ?       Chloroplast (Potential).
FT   CHAIN         ?    428       Delta-aminolevulinic acid dehydratase,
FT                                chloroplastic.
FT                                /FTId=PRO_0000013316.
FT   REGION      215    233       Magnesium-binding (By similarity).
FT   ACT_SITE    347    347       By similarity.
SQ   SEQUENCE   428 AA;  46213 MW;  2E3F6C37109816F7 CRC64;
     MASTVPFSPA KVQMFQATNC HGHAGFGSSF AVPRTGPRPR SVAVRVSSEQ EAAATVRAPS
     GRSIEECEAD AVAGRFPAPS CVCQTPKAPD GTPEIRPLDM AKRPRRNRRS PALRAAFQET
     SISPANLVLP LFIHEGEEDA PIGAMPGCFR LGWQHGLLAE VYKARDVGVN SFVLFPKVPD
     ALKSPTGVEA YNDNGLVPRT IRLLKDKFPD IIVYTDVALD PYSSDGHDGI VRKDGVILND
     ETVYQLCKQA VSQARAGADV VSPSNMMDGR VGAIRSALDA EGFNDVSIMS YTAKYASSFY
     GPFREALDSN PRFGDKKTYQ MNPANYREAL LETAADEAEG ADILLVKPGL PYLDIIRLSR
     DNSALPIAAY QVSGEYSMIK AGGALNMIDE EKVMMESLMC LRRAGADVIL TYFARQPPAV
     LCGMGTAK
//