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Q42836

- HEM2_HORVU

UniProt

Q42836 - HEM2_HORVU

Protein

Delta-aminolevulinic acid dehydratase, chloroplastic

Gene

HEMB

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen By similarity.By similarity

    Catalytic activityi

    2 5-aminolevulinate = porphobilinogen + 2 H2O.

    Cofactori

    Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei294 – 2941Schiff-base intermediate with substrateBy similarity
    Binding sitei304 – 3041Substrate 1By similarity
    Binding sitei316 – 3161Substrate 1By similarity
    Metal bindingi332 – 3321MagnesiumBy similarity
    Active sitei347 – 3471Schiff-base intermediate with substrateBy similarity
    Binding sitei373 – 3731Substrate 2By similarity
    Binding sitei412 – 4121Substrate 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. porphobilinogen synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. chlorophyll biosynthetic process Source: UniProtKB-KW
    2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00251; UER00318.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
    Short name:
    ALADH
    Alternative name(s):
    Porphobilinogen synthase
    Gene namesi
    Name:HEMB
    Synonyms:ALAD
    OrganismiHordeum vulgare (Barley)
    Taxonomic identifieri4513 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

    Organism-specific databases

    GrameneiQ42836.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 428Delta-aminolevulinic acid dehydratase, chloroplasticPRO_0000013316
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PRIDEiQ42836.

    Expressioni

    Gene expression databases

    GenevestigatoriQ42836.

    Interactioni

    Subunit structurei

    Homooctamer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ42836.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ALADH family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view]
    PANTHERiPTHR11458. PTHR11458. 1 hit.
    PfamiPF00490. ALAD. 1 hit.
    [Graphical view]
    PRINTSiPR00144. DALDHYDRTASE.
    SMARTiSM01004. ALAD. 1 hit.
    [Graphical view]
    PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q42836-1 [UniParc]FASTAAdd to Basket

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    MASTVPFSPA KVQMFQATNC HGHAGFGSSF AVPRTGPRPR SVAVRVSSEQ    50
    EAAATVRAPS GRSIEECEAD AVAGRFPAPS CVCQTPKAPD GTPEIRPLDM 100
    AKRPRRNRRS PALRAAFQET SISPANLVLP LFIHEGEEDA PIGAMPGCFR 150
    LGWQHGLLAE VYKARDVGVN SFVLFPKVPD ALKSPTGVEA YNDNGLVPRT 200
    IRLLKDKFPD IIVYTDVALD PYSSDGHDGI VRKDGVILND ETVYQLCKQA 250
    VSQARAGADV VSPSNMMDGR VGAIRSALDA EGFNDVSIMS YTAKYASSFY 300
    GPFREALDSN PRFGDKKTYQ MNPANYREAL LETAADEAEG ADILLVKPGL 350
    PYLDIIRLSR DNSALPIAAY QVSGEYSMIK AGGALNMIDE EKVMMESLMC 400
    LRRAGADVIL TYFARQPPAV LCGMGTAK 428
    Length:428
    Mass (Da):46,213
    Last modified:November 1, 1996 - v1
    Checksum:i2E3F6C37109816F7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92402 mRNA. Translation: CAA63139.1.
    PIRiT04472.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92402 mRNA. Translation: CAA63139.1 .
    PIRi T04472.

    3D structure databases

    ProteinModelPortali Q42836.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q42836.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Gramenei Q42836.

    Enzyme and pathway databases

    UniPathwayi UPA00251 ; UER00318 .

    Gene expression databases

    Genevestigatori Q42836.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR001731. Porphobilinogen_synth.
    [Graphical view ]
    PANTHERi PTHR11458. PTHR11458. 1 hit.
    Pfami PF00490. ALAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00144. DALDHYDRTASE.
    SMARTi SM01004. ALAD. 1 hit.
    [Graphical view ]
    PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Bougri O.
      Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Bonus.

    Entry informationi

    Entry nameiHEM2_HORVU
    AccessioniPrimary (citable) accession number: Q42836
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3