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Protein

Delta-aminolevulinic acid dehydratase, chloroplastic

Gene

HEMB

Organism
Hordeum vulgare (Barley)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator.By similarity

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Delta-aminolevulinic acid dehydratase, chloroplastic (HEMB)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Uroporphyrinogen decarboxylase (DCUP)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes coproporphyrinogen-III from 5-aminolevulinate, the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei294Schiff-base intermediate with substrateBy similarity1
Binding sitei304Substrate 1By similarity1
Binding sitei316Substrate 1By similarity1
Metal bindingi332MagnesiumBy similarity1
Active sitei347Schiff-base intermediate with substrateBy similarity1
Binding sitei373Substrate 2By similarity1
Binding sitei412Substrate 2By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Lyase
Biological processChlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEMB
Synonyms:ALAD
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBOP cladePooideaeTriticodaeTriticeaeHordeinaeHordeum

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000013316? – 428Delta-aminolevulinic acid dehydratase, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Proteomic databases

PRIDEiQ42836

Expressioni

Gene expression databases

ExpressionAtlasiQ42836 baseline and differential

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ42836
SMRiQ42836
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2794 Eukaryota
COG0113 LUCA

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR001731 ALAD
IPR030656 ALAD_AS
IPR013785 Aldolase_TIM
PANTHERiPTHR11458 PTHR11458, 1 hit
PfamiView protein in Pfam
PF00490 ALAD, 1 hit
PRINTSiPR00144 DALDHYDRTASE
SMARTiView protein in SMART
SM01004 ALAD, 1 hit
PROSITEiView protein in PROSITE
PS00169 D_ALA_DEHYDRATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42836-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASTVPFSPA KVQMFQATNC HGHAGFGSSF AVPRTGPRPR SVAVRVSSEQ
60 70 80 90 100
EAAATVRAPS GRSIEECEAD AVAGRFPAPS CVCQTPKAPD GTPEIRPLDM
110 120 130 140 150
AKRPRRNRRS PALRAAFQET SISPANLVLP LFIHEGEEDA PIGAMPGCFR
160 170 180 190 200
LGWQHGLLAE VYKARDVGVN SFVLFPKVPD ALKSPTGVEA YNDNGLVPRT
210 220 230 240 250
IRLLKDKFPD IIVYTDVALD PYSSDGHDGI VRKDGVILND ETVYQLCKQA
260 270 280 290 300
VSQARAGADV VSPSNMMDGR VGAIRSALDA EGFNDVSIMS YTAKYASSFY
310 320 330 340 350
GPFREALDSN PRFGDKKTYQ MNPANYREAL LETAADEAEG ADILLVKPGL
360 370 380 390 400
PYLDIIRLSR DNSALPIAAY QVSGEYSMIK AGGALNMIDE EKVMMESLMC
410 420
LRRAGADVIL TYFARQPPAV LCGMGTAK
Length:428
Mass (Da):46,213
Last modified:November 1, 1996 - v1
Checksum:i2E3F6C37109816F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92402 mRNA Translation: CAA63139.1
PIRiT04472

Similar proteinsi

Entry informationi

Entry nameiHEM2_HORVU
AccessioniPrimary (citable) accession number: Q42836
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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