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Q42836

- HEM2_HORVU

UniProt

Q42836 - HEM2_HORVU

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Protein

Delta-aminolevulinic acid dehydratase, chloroplastic

Gene

HEMB

Organism
Hordeum vulgare (Barley)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen (By similarity).By similarity

Catalytic activityi

2 5-aminolevulinate = porphobilinogen + 2 H2O.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per monomer. The first magnesium ion is required for catalysis. The second functions as allosteric activator.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei294 – 2941Schiff-base intermediate with substrateBy similarity
Binding sitei304 – 3041Substrate 1By similarity
Binding sitei316 – 3161Substrate 1By similarity
Metal bindingi332 – 3321MagnesiumBy similarity
Active sitei347 – 3471Schiff-base intermediate with substrateBy similarity
Binding sitei373 – 3731Substrate 2By similarity
Binding sitei412 – 4121Substrate 2By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. porphobilinogen synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. chlorophyll biosynthetic process Source: UniProtKB-KW
  2. protoporphyrinogen IX biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Chlorophyll biosynthesis, Heme biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00318.

Names & Taxonomyi

Protein namesi
Recommended name:
Delta-aminolevulinic acid dehydratase, chloroplastic (EC:4.2.1.24)
Short name:
ALADH
Alternative name(s):
Porphobilinogen synthase
Gene namesi
Name:HEMB
Synonyms:ALAD
OrganismiHordeum vulgare (Barley)
Taxonomic identifieri4513 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladePooideaeTriticeaeHordeum

Organism-specific databases

GrameneiQ42836.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 428Delta-aminolevulinic acid dehydratase, chloroplasticPRO_0000013316
Transit peptidei1 – ?ChloroplastSequence Analysis

Proteomic databases

PRIDEiQ42836.

Expressioni

Gene expression databases

GenevestigatoriQ42836.

Interactioni

Subunit structurei

Homooctamer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ42836.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ALAD family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view]
PANTHERiPTHR11458. PTHR11458. 1 hit.
PfamiPF00490. ALAD. 1 hit.
[Graphical view]
PRINTSiPR00144. DALDHYDRTASE.
SMARTiSM01004. ALAD. 1 hit.
[Graphical view]
PROSITEiPS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42836-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASTVPFSPA KVQMFQATNC HGHAGFGSSF AVPRTGPRPR SVAVRVSSEQ
60 70 80 90 100
EAAATVRAPS GRSIEECEAD AVAGRFPAPS CVCQTPKAPD GTPEIRPLDM
110 120 130 140 150
AKRPRRNRRS PALRAAFQET SISPANLVLP LFIHEGEEDA PIGAMPGCFR
160 170 180 190 200
LGWQHGLLAE VYKARDVGVN SFVLFPKVPD ALKSPTGVEA YNDNGLVPRT
210 220 230 240 250
IRLLKDKFPD IIVYTDVALD PYSSDGHDGI VRKDGVILND ETVYQLCKQA
260 270 280 290 300
VSQARAGADV VSPSNMMDGR VGAIRSALDA EGFNDVSIMS YTAKYASSFY
310 320 330 340 350
GPFREALDSN PRFGDKKTYQ MNPANYREAL LETAADEAEG ADILLVKPGL
360 370 380 390 400
PYLDIIRLSR DNSALPIAAY QVSGEYSMIK AGGALNMIDE EKVMMESLMC
410 420
LRRAGADVIL TYFARQPPAV LCGMGTAK
Length:428
Mass (Da):46,213
Last modified:November 1, 1996 - v1
Checksum:i2E3F6C37109816F7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92402 mRNA. Translation: CAA63139.1.
PIRiT04472.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92402 mRNA. Translation: CAA63139.1 .
PIRi T04472.

3D structure databases

ProteinModelPortali Q42836.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PRIDEi Q42836.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Gramenei Q42836.

Enzyme and pathway databases

UniPathwayi UPA00251 ; UER00318 .

Gene expression databases

Genevestigatori Q42836.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
InterProi IPR013785. Aldolase_TIM.
IPR001731. Porphobilinogen_synth.
[Graphical view ]
PANTHERi PTHR11458. PTHR11458. 1 hit.
Pfami PF00490. ALAD. 1 hit.
[Graphical view ]
PRINTSi PR00144. DALDHYDRTASE.
SMARTi SM01004. ALAD. 1 hit.
[Graphical view ]
PROSITEi PS00169. D_ALA_DEHYDRATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Bougri O.
    Submitted (OCT-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Bonus.

Entry informationi

Entry nameiHEM2_HORVU
AccessioniPrimary (citable) accession number: Q42836
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: November 26, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3