ID   KPYC_SOYBN              Reviewed;         511 AA.
AC   Q42806;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Pyruvate kinase, cytosolic isozyme;
DE            Short=PK;
DE            EC=2.7.1.40;
OS   Glycine max (Soybean).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Fabales; Fabaceae; Papilionoideae; Phaseoleae;
OC   Glycine.
OX   NCBI_TaxID=3847;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=94105349; PubMed=8278551; DOI=10.1104/pp.102.4.1345;
RA   Ma H., McMullen M.D., Finer J.J.;
RT   "A pyruvate kinase cDNA from soybean somatic embryos.";
RL   Plant Physiol. 102:1345-1345(1993).
CC   -!- CATALYTIC ACTIVITY: ATP + pyruvate = ADP + phosphoenolpyruvate.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- COFACTOR: Potassium (By similarity).
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 5/5.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the pyruvate kinase family.
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DR   EMBL; L08632; AAA17000.1; -; mRNA.
DR   PIR; T07787; T07787.
DR   UniGene; Gma.4073; -.
DR   HSSP; P14178; 1E0T.
DR   BRENDA; 2.7.1.40; 299.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR   GO; GO:0004743; F:pyruvate kinase activity; IEA:EC.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   InterPro; IPR001697; Pyr_Knase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase_cat.
DR   InterPro; IPR015794; Pyrv_Knase_a/b.
DR   InterPro; IPR018209; Pyrv_Knase_AS.
DR   InterPro; IPR015793; Pyrv_Knase_brl.
DR   Gene3D; G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1.
DR   Gene3D; G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1.
DR   PANTHER; PTHR11817; Pyruvate_kinase; 1.
DR   Pfam; PF00224; PK; 1.
DR   Pfam; PF02887; PK_C; 1.
DR   PRINTS; PR01050; PYRUVTKNASE.
DR   ProDom; PD001009; Pyruvate_kinase; 2.
DR   TIGRFAMs; TIGR01064; pyruv_kin; 1.
DR   PROSITE; PS00110; PYRUVATE_KINASE; 1.
PE   2: Evidence at transcript level;
KW   ATP-binding; Cytoplasm; Glycolysis; Kinase; Magnesium; Metal-binding;
KW   Nucleotide-binding; Pyruvate; Transferase.
FT   CHAIN         1    511       Pyruvate kinase, cytosolic isozyme.
FT                                /FTId=PRO_0000112125.
FT   ACT_SITE    241    241       By similarity.
FT   METAL       243    243       Magnesium (Potential).
FT   METAL       264    264       Magnesium (Potential).
FT   METAL       265    265       Magnesium (Potential).
SQ   SEQUENCE   511 AA;  55302 MW;  BF0C06DAC6FD95F1 CRC64;
     MANIDIEGIL KQQQPYDGRV PKTKIVCTLG PASRSVEMTE KLLRAGMNVA RFNFSHGTHD
     YHQETLNNLK TAMHNTGILC AVMLDTKGPE IRTGFLKDGK PIQLKEGQEV TITTDYDIKG
     DPEMISMSYK KLPVHLKPGN TILCSDGTIT LTVLSCDPDA GTVRCRCENT ATLGERKNVN
     LPGVVVDLPT LTEKDKEDIL GWGVPNKIDM IALSFVRKGS DLVNVRKVLG PHAKNIQLMS
     KVENQEGVLN FDEILRETDA FMVARGDLGM EIPVEKIFLA QKMMIYKCNL VGKPVVTATQ
     MLESMIKSPR PTRAEATDVA NAVLDGTDCV MLSGESAAGA YPELAVKIMA RICIEAESSL
     DYGAIFKEMI RSTPLPMSPL ESLASSAVRT ANKAKAKLIV VLTRGGSTAK LVAKYRPAVP
     ILSVVVPVLS TDSFDWTCSD ETPARHSLIY RGLIPILGEG SAKATDAEST EVILEAALKS
     ATERALCKPG DAVVALHRIG AASVIKICIV K
//