Q42806 (KPYC_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Pyruvate kinase, cytosolic isozyme Short name=PK EC=2.7.1.40
Organism	Glycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Pyruvate kinase, cytosolic isozyme		PRO_0000112125
Sites
	Metal binding	53	1	Potassium By similarity
	Metal binding	55	1	Potassium By similarity
	Metal binding	85	1	Potassium By similarity
	Metal binding	86	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	243	1	Magnesium Potential
	Metal binding	267	1	Magnesium By similarity
	Binding site	51	1	Substrate By similarity
	Binding site	266	1	Substrate; via amide nitrogen By similarity
	Binding site	267	1	Substrate; via amide nitrogen By similarity
	Binding site	299	1	Substrate By similarity
	Site	241	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42806 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BF0C06DAC6FD95F1	FASTA	511	55,302
	10 20 30 40 50 60 MANIDIEGIL KQQQPYDGRV PKTKIVCTLG PASRSVEMTE KLLRAGMNVA RFNFSHGTHD 70 80 90 100 110 120 YHQETLNNLK TAMHNTGILC AVMLDTKGPE IRTGFLKDGK PIQLKEGQEV TITTDYDIKG 130 140 150 160 170 180 DPEMISMSYK KLPVHLKPGN TILCSDGTIT LTVLSCDPDA GTVRCRCENT ATLGERKNVN 190 200 210 220 230 240 LPGVVVDLPT LTEKDKEDIL GWGVPNKIDM IALSFVRKGS DLVNVRKVLG PHAKNIQLMS 250 260 270 280 290 300 KVENQEGVLN FDEILRETDA FMVARGDLGM EIPVEKIFLA QKMMIYKCNL VGKPVVTATQ 310 320 330 340 350 360 MLESMIKSPR PTRAEATDVA NAVLDGTDCV MLSGESAAGA YPELAVKIMA RICIEAESSL 370 380 390 400 410 420 DYGAIFKEMI RSTPLPMSPL ESLASSAVRT ANKAKAKLIV VLTRGGSTAK LVAKYRPAVP 430 440 450 460 470 480 ILSVVVPVLS TDSFDWTCSD ETPARHSLIY RGLIPILGEG SAKATDAEST EVILEAALKS 490 500 510 ATERALCKPG DAVVALHRIG AASVIKICIV K

References

[1]	"A pyruvate kinase cDNA from soybean somatic embryos." Ma H., McMullen M.D., Finer J.J. Plant Physiol. 102:1345-1345(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L08632 mRNA. Translation: AAA17000.1.
PIR	T07787.
RefSeq	NP_001237968.1. NM_001251039.1.
UniGene	Gma.4073.
3D structure databases
ProteinModelPortal	Q42806.
ModBase	Search...
Proteomic databases
ProMEX	Q42806.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	GLYMA05G09310.1; GLYMA05G09310.1; GLYMA05G09310. GLYMA05G09310.2; GLYMA05G09310.2; GLYMA05G09310.
GeneID	547791.
KEGG	gmx:547791.
Phylogenomic databases
KO	K00873.
Enzyme and pathway databases
UniPathway	UPA00109; UER00188.
Gene expression databases
Genevestigator	Q42806.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYC_SOYBN

Accession

Primary (citable) accession number: Q42806

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program