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Protein

Pyruvate kinase, cytosolic isozyme

Gene
N/A
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511SubstrateBy similarity
Metal bindingi53 – 531PotassiumBy similarity
Metal bindingi55 – 551PotassiumBy similarity
Metal bindingi85 – 851PotassiumBy similarity
Metal bindingi86 – 861Potassium; via carbonyl oxygenBy similarity
Sitei241 – 2411Transition state stabilizerBy similarity
Metal bindingi243 – 2431MagnesiumSequence Analysis
Binding sitei266 – 2661Substrate; via amide nitrogenBy similarity
Metal bindingi267 – 2671MagnesiumBy similarity
Binding sitei267 – 2671Substrate; via amide nitrogenBy similarity
Binding sitei299 – 2991SubstrateBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. magnesium ion binding Source: InterPro
  3. potassium ion binding Source: InterPro
  4. pyruvate kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. glycolytic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase, cytosolic isozyme (EC:2.7.1.40)
Short name:
PK
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827 Componenti: Chromosome 5

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 511511Pyruvate kinase, cytosolic isozymePRO_0000112125Add
BLAST

Proteomic databases

PRIDEiQ42806.
ProMEXiQ42806.

Expressioni

Gene expression databases

GenevestigatoriQ42806.

Interactioni

Subunit structurei

Homotetramer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ42806.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

InParanoidiQ42806.
KOiK00873.
OMAiPRTKLVC.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q42806-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANIDIEGIL KQQQPYDGRV PKTKIVCTLG PASRSVEMTE KLLRAGMNVA
60 70 80 90 100
RFNFSHGTHD YHQETLNNLK TAMHNTGILC AVMLDTKGPE IRTGFLKDGK
110 120 130 140 150
PIQLKEGQEV TITTDYDIKG DPEMISMSYK KLPVHLKPGN TILCSDGTIT
160 170 180 190 200
LTVLSCDPDA GTVRCRCENT ATLGERKNVN LPGVVVDLPT LTEKDKEDIL
210 220 230 240 250
GWGVPNKIDM IALSFVRKGS DLVNVRKVLG PHAKNIQLMS KVENQEGVLN
260 270 280 290 300
FDEILRETDA FMVARGDLGM EIPVEKIFLA QKMMIYKCNL VGKPVVTATQ
310 320 330 340 350
MLESMIKSPR PTRAEATDVA NAVLDGTDCV MLSGESAAGA YPELAVKIMA
360 370 380 390 400
RICIEAESSL DYGAIFKEMI RSTPLPMSPL ESLASSAVRT ANKAKAKLIV
410 420 430 440 450
VLTRGGSTAK LVAKYRPAVP ILSVVVPVLS TDSFDWTCSD ETPARHSLIY
460 470 480 490 500
RGLIPILGEG SAKATDAEST EVILEAALKS ATERALCKPG DAVVALHRIG
510
AASVIKICIV K
Length:511
Mass (Da):55,302
Last modified:November 1, 1996 - v1
Checksum:iBF0C06DAC6FD95F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08632 mRNA. Translation: AAA17000.1.
PIRiT07787.
RefSeqiNP_001237968.1. NM_001251039.1.
UniGeneiGma.4073.

Genome annotation databases

EnsemblPlantsiGLYMA05G09310.1; GLYMA05G09310.1; GLYMA05G09310.
GLYMA05G09310.2; GLYMA05G09310.2; GLYMA05G09310.
GeneIDi547791.
KEGGigmx:547791.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08632 mRNA. Translation: AAA17000.1.
PIRiT07787.
RefSeqiNP_001237968.1. NM_001251039.1.
UniGeneiGma.4073.

3D structure databases

ProteinModelPortaliQ42806.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ42806.
ProMEXiQ42806.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGLYMA05G09310.1; GLYMA05G09310.1; GLYMA05G09310.
GLYMA05G09310.2; GLYMA05G09310.2; GLYMA05G09310.
GeneIDi547791.
KEGGigmx:547791.

Phylogenomic databases

InParanoidiQ42806.
KOiK00873.
OMAiPRTKLVC.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Gene expression databases

GenevestigatoriQ42806.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A pyruvate kinase cDNA from soybean somatic embryos."
    Ma H., McMullen M.D., Finer J.J.
    Plant Physiol. 102:1345-1345(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiKPYC_SOYBN
AccessioniPrimary (citable) accession number: Q42806
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 29, 2015
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.