Reviewed, UniProtKB/Swiss-Prot Q42806 (KPYC_SOYBN)

Names and origin

Protein names	Recommended name:     Pyruvate kinase, cytosolic isozyme       Short name=PK     EC=2.7.1.40
Organism	Glycine max (Soybean)
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	511 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 511	511	Pyruvate kinase, cytosolic isozyme		PRO_0000112125
Sites
	Active site	241	1	By similarity
	Metal binding	243	1	Magnesium Potential
	Metal binding	264	1	Magnesium Potential
	Metal binding	265	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q42806-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BF0C06DAC6FD95F1	FASTA	511	55,302
	10 20 30 40 50 60 MANIDIEGIL KQQQPYDGRV PKTKIVCTLG PASRSVEMTE KLLRAGMNVA RFNFSHGTHD 70 80 90 100 110 120 YHQETLNNLK TAMHNTGILC AVMLDTKGPE IRTGFLKDGK PIQLKEGQEV TITTDYDIKG 130 140 150 160 170 180 DPEMISMSYK KLPVHLKPGN TILCSDGTIT LTVLSCDPDA GTVRCRCENT ATLGERKNVN 190 200 210 220 230 240 LPGVVVDLPT LTEKDKEDIL GWGVPNKIDM IALSFVRKGS DLVNVRKVLG PHAKNIQLMS 250 260 270 280 290 300 KVENQEGVLN FDEILRETDA FMVARGDLGM EIPVEKIFLA QKMMIYKCNL VGKPVVTATQ 310 320 330 340 350 360 MLESMIKSPR PTRAEATDVA NAVLDGTDCV MLSGESAAGA YPELAVKIMA RICIEAESSL 370 380 390 400 410 420 DYGAIFKEMI RSTPLPMSPL ESLASSAVRT ANKAKAKLIV VLTRGGSTAK LVAKYRPAVP 430 440 450 460 470 480 ILSVVVPVLS TDSFDWTCSD ETPARHSLIY RGLIPILGEG SAKATDAEST EVILEAALKS 490 500 510 ATERALCKPG DAVVALHRIG AASVIKICIV K

References

[1]	"A pyruvate kinase cDNA from soybean somatic embryos." Ma H., McMullen M.D., Finer J.J. Plant Physiol. 102:1345-1345(1993) [PubMed: 8278551] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	L08632 mRNA. Translation: AAA17000.1.
PIR	T07787.
UniGene	Gma.4073
3D structure databases
HSSP	HSSP built from PDB template 1E0T based on UniProtKB P14178.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 299.
Family and domain databases
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYC_SOYBN

Accession

Primary (citable) accession number: Q42806

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)