Q42805 (PUR3_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylglycinamide formyltransferase, chloroplastic
EC=2.1.2.2

Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART
GMpurN

Gene names

Name:	PUR3
Synonyms:	PURN

Organism

Glycine max (Soybean) (Glycine hispida)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	295 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Highly expressed in young and mature nodules but weakly expressed in roots and leaves.
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: InterPro phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 62	62	Chloroplast Potential
	Chain	63 – 295	233	Phosphoribosylglycinamide formyltransferase, chloroplastic		PRO_0000029877
Regions
	Region	96 – 98	3	5'-phosphoribosylglycinamide binding By similarity
	Region	171 – 174	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	188	1	Proton donor By similarity
	Binding site	230	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	259	1	5'-phosphoribosylglycinamide By similarity
	Site	230	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42805 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 44908F208E95A752	FASTA	295	32,823
	10 20 30 40 50 60 MSVPSIPIVK QQFSPKFPRL PSSSLYPSSQ SQNLNVPSGA FHPISIVHKE VCSSSCKRIW 70 80 90 100 110 120 CSSSSSSTAE PKEGHEVRAQ VTVRRKKLGV FVSGGGTNFR AIHEATKRGS LHGDVLVLVT 130 140 150 160 170 180 NKSDCGGAEY ARNNGIPVIL YHISKDESNG SDLVDTLRKF EVDFILLAGY LNLYQWNDPS 190 200 210 220 230 240 LQKIYIQHSS ITSSSFWRQG IHGMKVHKAV IASGARFSGP TIHFVDEHYD TGRILAQRVV 250 260 270 280 290 PVQANDTVEE LAARVLKEEH QLYVEVVEAL CEERVVWRQD GVPLIQSKEN PNEFR

References

[1]

"Isolation of cDNAs encoding two purine biosynthetic enzymes of soybean and expression of the corresponding transcripts in roots and root nodules." Schnorr K.M., Laloue M., Hirel B. Plant Mol. Biol. 32:751-757(1996) [PubMed: 8980527] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X96865 mRNA. Translation: CAA65608.1.
PIR	T07781.
3D structure databases
ProteinModelPortal	Q42805.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Gene expression databases
Genevestigator	Q42805.
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR001555. GART_AS. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF53328. formyl_transf. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_SOYBN

Accession

Primary (citable) accession number: Q42805

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 8, 2000
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program