Reviewed, UniProtKB/Swiss-Prot Q42797 (TCMO_SOYBN)

Names and origin

Protein names

Recommended name:
    Trans-cinnamate 4-monooxygenase
    EC=1.14.13.11
Alternative name(s):
    Cinnamic acid 4-hydroxylase
      Short name=CA4H
      Short name=C4H
    P450C4H
    Cytochrome P450 73

Gene names

Name:

CYP73A11

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	506 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Controls carbon flux to pigments essential for pollination or UV protection, to numerous pytoalexins synthesized by plants when challenged by pathogens, and to lignins.
Catalytic activity	Trans-cinnamate + NADPH + O2 = 4-hydroxycinnamate + NADP+ + H2O.
Cofactor	Heme group By similarity.
Pathway	Phenylpropanoid metabolism; trans-4-coumarate biosynthesis; trans-4-coumarate from trans-cinnamate: step 1/1.
Induction	By fungal elicitor.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro trans-cinnamate 4-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 506	506	Trans-cinnamate 4-monooxygenase		PRO_0000052254
Sites
	Metal binding	448	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42797-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 0BA3F890EEE4FE19	FASTA	506	58,011
	10 20 30 40 50 60 MDLLLLEKTL IGLFLAAVVA IAVSTLRGRK FKLPPGPLPV PIFGNWLQVG DDLNHRNLTD 70 80 90 100 110 120 LAKKFGDIFL LRMGQRNLVV VSSPELAKEV LHTQGVEFGS RTRNVVFDIF TGKGQDMVFT 130 140 150 160 170 180 VYGEHWRKMR RIMTVPFFTN KVVQQYRHGW ESEAAAVVED VKKNPDAAVS GTVIRRRLQL 190 200 210 220 230 240 MMYNNMYRIM FDRRFESEED PIFQRLRALN GERSRLAQSF EYNYGDFIPI LRPFLKGYLK 250 260 270 280 290 300 ICKEVKETRL KLFKDYFVDE RKKLGSTKST NNNNELKCAI DHILDAQRKG EINEDNVLYI 310 320 330 340 350 360 VENINVAAIE TTLWSIEWGI AELVNHPEIQ QKLRDEIDRV LGAGHQVTEP DIQKLPYLQA 370 380 390 400 410 420 VVKETLRLRM AIPLLVPHMN LHDAKLGGYD IPAESKILVN AWWLANNPAH WKKPEEFRPE 430 440 450 460 470 480 RFFEEESLVE ANGNDFRYLP FGVGRRSCPG IILALPILGI TLGRLVQNFE LLPPPGQSQI 490 500 DTSEKGGQFS LHILKHSTIV AKPRSF

References

[1]	"Identification of elicitor-induced cytochrome P450s of soybean (Glycine max L.) using differential display of mRNA." Schopfer C.R., Ebel J. Mol. Gen. Genet. 258:315-322(1998) [PubMed: 9648734] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Harosoy 63.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X92437 mRNA. Translation: CAA63172.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.13.11. 299.
Gene expression databases
Genevestigator	Q42797.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TCMO_SOYBN

Accession

Primary (citable) accession number: Q42797

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	October 13, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)