Reviewed, UniProtKB/Swiss-Prot Q42796 (F16P1_SOYBN)

Names and origin

Protein names

Recommended name:
    Fructose-1,6-bisphosphatase, chloroplastic
      Short name=FBPase
    EC=3.1.3.11
Alternative name(s):
    D-fructose-1,6-bisphosphate 1-phosphohydrolase

Gene names

Name:

FBP

Organism

Glycine max (Soybean)

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Homotetramer By similarity.
Subcellular location	Plastid › chloroplast.
Induction	Light activation through pH changes, Mg2+ levels and also by light-modulated reduction of essential disulfide groups via the ferredoxin-thioredoxin f system By similarity.
Miscellaneous	In plants there are two FBPase isozymes: one in the cytosol and the other in the chloroplast.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Calvin cycle Carbohydrate metabolism
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Chloroplast Potential
	Chain	51 – 402	352	Fructose-1,6-bisphosphatase, chloroplastic		PRO_0000008819
Regions
	Region	180 – 183	4	Substrate binding By similarity
Sites
	Metal binding	127	1	Magnesium 1 By similarity
	Metal binding	156	1	Magnesium 1 By similarity
	Metal binding	156	1	Magnesium 2 By similarity
	Metal binding	177	1	Magnesium 2 By similarity
	Metal binding	177	1	Magnesium 3 By similarity
	Metal binding	179	1	Magnesium 2; via carbonyl oxygen By similarity
	Metal binding	180	1	Magnesium 3 By similarity
	Metal binding	353	1	Magnesium 3 By similarity
	Binding site	285	1	Substrate By similarity
	Binding site	317	1	Substrate By similarity
	Binding site	335	1	Substrate By similarity
	Binding site	337	1	Substrate By similarity
	Binding site	347	1	Substrate By similarity
Amino acid modifications
	Disulfide bond	221 ↔ 226		Redox-active (light-modulated) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42796-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 2EC4876454C59A3B	FASTA	402	43,897
	10 20 30 40 50 60 MAAATASTQL IFSKPCSPSR LCPFQLCVFD TKQVLSSGRR RHVGGSGVRC MAVGEAATTG 70 80 90 100 110 120 TKKRSGYELQ TLTSWLLKQE QAGVIDAELT IVLSSISMAC KQIASLVQRA NISNLTGVQG 130 140 150 160 170 180 AVNVQGEDQK KLDVVSNEVF SNCLRSSGRT GIIASEEEDV PVAVEESYSG NYIVVFDPLD 190 200 210 220 230 240 GSSNIDAAAS TGSNFWIYSP NDECLADIDD DPTLDTTEQR CIVNVCQPGS NLLAAGYCMY 250 260 270 280 290 300 SSSIIFVLTL GNGVFVFTLD PMYGEFVLTQ ENLQIPRAGK IYAFNEGNYQ LWDEKLKKYI 310 320 330 340 350 360 DDLKDPGQSG KPYSARYIGS LVGDFHRTLL YGGIYGYPRD KKSKNGKLRL LYECAPINFI 370 380 390 400 VEQAGGKGTD GLQVLRLQGT EIHQRVPLYI GEEVEKVEKY LA

References

[1]	"Molecular characterization of a cDNA encoding chloroplastic fructose-1,6-bisphosphatase from soybean (Glycine max L.)." Jeon Y.H., Bhoo S.H., Hahn T.R. Mol. Cells 8:113-116(1998) [PubMed: 9571641] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Jinhung. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L34841 mRNA. Translation: AAA33956.1.
PIR	T07134.
UniGene	Gma.33674
3D structure databases
SMR	Q42796. Positions 63-402.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.1.3.11. 299.
Gene expression databases
Genevestigator	Q42796.
Family and domain databases
InterPro	IPR000146. Fructose_bisphosphatase. [Graphical view]
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16P1_SOYBN

Accession

Primary (citable) accession number: Q42796

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	February 9, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)