Reviewed, UniProtKB/Swiss-Prot Q42713 (PLSB_CARTI)

Names and origin

Protein names	Recommended name:     Glycerol-3-phosphate acyltransferase, chloroplastic       Short name=GPAT     EC=2.3.1.15
Organism	Carthamus tinctorius (Safflower)
Taxonomic identifier	4222 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Carduoideae › Cardueae › Carthamus

Protein attributes

Sequence length	463 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Esterifies acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate. The enzyme from chilling-resistant plants discriminates against non-fluid palmitic acid and selects oleic acid whereas the enzyme from sensitive plants accepts both fatty acids.
Catalytic activity	Acyl-CoA + sn-glycerol 3-phosphate = CoA + 1-acyl-sn-glycerol 3-phosphate.
Pathway	Phospholipid metabolism; CDP-diacylglycerol biosynthesis; CDP-diacylglycerol from sn-glycerol 3-phosphate: step 1/3.
Subcellular location	Plastid › chloroplast stroma.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the GPAT/DAPAT family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 91	91	Chloroplast Potential
	Chain	92 – 463	372	Glycerol-3-phosphate acyltransferase, chloroplastic		PRO_0000024695
Regions
	Motif	229 – 234	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q42713-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 5792E933068A534D	FASTA	463	50,835
	10 20 30 40 50 60 MSIFFSPSSP TLFFSTTNAN PRVSPSSSPS SAFTPPLSSS RLRPILRGFP CLAFSAPANA 70 80 90 100 110 120 AHGTAETVHG NKWPSPSSSS SAATQPSAGS DHGHSRTFID ARSEQDLLSG IQRELEAGTL 130 140 150 160 170 180 PKHIAQAMEE LYQNYKNAVL QSAAPHAEDI VLSNMRVAFD RMFLDVKEPF EFSPYHEAIL 190 200 210 220 230 240 EPFNYYMFGQ NYIRPLVNFR ESYVGNVSVF GVMEEQLKQG DKVVLISNHQ TEADPAVIAL 250 260 270 280 290 300 MLETTNPHIS ENIIYVAGDR VITDPLCKPF SMGRNLLCVY SKKHMNDVPE LAEMKKRSNT 310 320 330 340 350 360 RSLKGRMALL LRGGSKIIWI APSGGRDRPD PITNQWFPAP FDATSLDNMR RLVDHAGLVG 370 380 390 400 410 420 HIYPLAILCH DIMPPPLQVE KEIGEKSWIS FHGTGISVAP EINFQEVTAS CGSPEEAKAA 430 440 450 460 YSQALYDSVC EQYKVLHSAV HGGKGLEAST PSVSLSQPLQ FLD

References

[1]	"Nucleotide sequence of a cDNA from Carthamus tinctorius encoding a glycerol-3-phosphate acyl transferase." Bhella R.S., Mackenzie S.L. Plant Physiol. 106:1713-1714(1994) [PubMed: 7846182] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Cotyledon.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L33841 mRNA. Translation: AAA74319.1.
3D structure databases
HSSP	HSSP built from PDB template 1K30 based on UniProtKB P10349.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.15. 66868.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR016222. Glycerol-3-P_O-acyltransferase. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
PIRSF	PIRSF000431. Glycerol-3-P_O-acyltransfrase. 1 hit.
SMART	SM00563. PlsC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PLSB_CARTI

Accession

Primary (citable) accession number: Q42713

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)