ID   MDARS_CUCSA             Reviewed;         434 AA.
AC   Q42711;
DT   19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 48.
DE   RecName: Full=Monodehydroascorbate reductase, seedling isozyme;
DE            Short=MDAR seedling;
DE            EC=1.6.5.4;
DE   AltName: Full=Ascorbate free radical reductase seedling;
DE            Short=AFR reductase seedling;
OS   Cucumis sativus (Cucumber).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids I; Cucurbitales; Cucurbitaceae; Cucumis.
OX   NCBI_TaxID=3659;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Seedling cotyledon;
RX   MEDLINE=94332398; PubMed=8055175;
RA   Sano S., Asada K.;
RT   "cDNA cloning of monodehydroascorbate radical reductase from Cucumber:
RT   a high degree of homology in terms of amino acid sequence between this
RT   enzyme and bacterial flavoenzymes.";
RL   Plant Cell Physiol. 35:425-437(1994).
CC   -!- FUNCTION: Catalyzes the conversion of monodehydroascorbate to
CC       ascorbate, oxidizing NADH in the process.
CC   -!- CATALYTIC ACTIVITY: NADH + 2 monodehydroascorbate = NAD(+) + 2
CC       ascorbate.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the FAD-dependent oxidoreductase family.
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DR   EMBL; D26392; BAA05408.1; -; mRNA.
DR   PIR; JU0182; JU0182.
DR   BRENDA; 1.6.5.4; 1241.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:FAD binding; IEA:InterPro.
DR   GO; GO:0016656; F:monodehydroascorbate reductase (NADH) activity; IEA:EC.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013027; FAD_pyr_nucl-diS_OxRdtase.
DR   InterPro; IPR001327; Pyr_OxRdtase_NAD_bd.
DR   Pfam; PF00070; Pyr_redox; 1.
DR   Pfam; PF07992; Pyr_redox_2; 1.
DR   PRINTS; PR00368; FADPNR.
DR   ProDom; PD000139; FAD_pyr_redox; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; FAD; Flavoprotein; NAD; Oxidoreductase;
KW   Redox-active center.
FT   CHAIN         1    434       Monodehydroascorbate reductase, seedling
FT                                isozyme.
FT                                /FTId=PRO_0000209140.
FT   NP_BIND       7     24       FAD (By similarity).
FT   NP_BIND     165    182       NAD (By similarity).
FT   NP_BIND     191    195       FAD (By similarity).
SQ   SEQUENCE   434 AA;  47416 MW;  302DA61F8C3E4E23 CRC64;
     MADETFKYVI LGGGVAAGYA AREFVKQGLN PGELAIISKE AVAPYERPAL SKAYLFPESP
     ARLPGFHVCV GSGGERLLPD WYKEKGIELI LSTEIVEADL PAKRLRSAHG KIYNYQTLII
     ATGSTVIKLS DFGVQGADAK NIFYLREIDD ADQLVEAIKA KENGKVVVVG GGYIGLELGA
     ALRINNFDVS MVYPEPWCMP RLFTPEIAAF YEGYYAQKGI TIIKGTVAVG FTVDTNGEVK
     EVKLKDGRVL EADIVVVGVG ARPLTSLFKG QIVEEKGGIK TDEFFKTSVP DVYAVGDVAT
     FPLKLYNELR RVEHVDHSRK SAEQAVKAIK ASEEGKAIEE YDYLPYFYSR SFDLSWQFYG
     DNVGDAVLFG DNSPDSATHK FGSYWIKDGK VVGAFLESGS PEENKAIAKV ARIQPSVESS
     DLLLKEGISF ASKV
//