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Protein

Monodehydroascorbate reductase, seedling isozyme

Gene
N/A
Organism
Cucumis sativus (Cucumber)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalyzes the conversion of monodehydroascorbate to ascorbate, oxidizing NADH in the process.

Catalytic activityi

NADH + 2 monodehydroascorbate = NAD+ + 2 ascorbate.By similarity

Cofactori

FADUniRule annotationCurated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40FADBy similarity1
Binding sitei47FADBy similarity1
Binding sitei52FADBy similarity1
Binding sitei95FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei195NADBy similarity1
Binding sitei201NADBy similarity1
Binding sitei201NADPBy similarity1
Binding sitei260NAD; via amide nitrogenBy similarity1
Binding sitei260NADP; via amide nitrogenBy similarity1
Binding sitei297FADBy similarity1
Binding sitei315FAD; via amide nitrogenBy similarity1
Binding sitei319AscorbateBy similarity1
Binding sitei348FAD; via carbonyl oxygenBy similarity1
Binding sitei348NAD; via carbonyl oxygenBy similarity1
Binding sitei348NADP; via carbonyl oxygenBy similarity1
Binding sitei350AscorbateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 16FADBy similarity4
Nucleotide bindingi146 – 147FADBy similarity2
Nucleotide bindingi171 – 177NADBy similarity7
Nucleotide bindingi173 – 177NADPBy similarity5
Nucleotide bindingi313 – 314NADBy similarity2
Nucleotide bindingi313 – 314NADPBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, NAD, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Monodehydroascorbate reductase, seedling isozyme (EC:1.6.5.4)
Short name:
MDAR seedling
Alternative name(s):
Ascorbate free radical reductase seedling
Short name:
AFR reductase seedling
OrganismiCucumis sativus (Cucumber)Imported
Taxonomic identifieri3659 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsCucurbitalesCucurbitaceaeBenincaseaeCucumis

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002091401 – 434Monodehydroascorbate reductase, seedling isozymeAdd BLAST434

Structurei

3D structure databases

ProteinModelPortaliQ42711
SMRiQ42711
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Redox-active center

Phylogenomic databases

KOiK08232

Family and domain databases

Gene3Di3.30.390.30, 1 hit
3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR016156 FAD/NAD-linked_Rdtase_dimer_sf
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
SUPFAMiSSF51905 SSF51905, 1 hit
SSF55424 SSF55424, 1 hit

Sequencei

Sequence statusi: Complete.

Q42711-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADETFKYVI LGGGVAAGYA AREFVKQGLN PGELAIISKE AVAPYERPAL
60 70 80 90 100
SKAYLFPESP ARLPGFHVCV GSGGERLLPD WYKEKGIELI LSTEIVEADL
110 120 130 140 150
PAKRLRSAHG KIYNYQTLII ATGSTVIKLS DFGVQGADAK NIFYLREIDD
160 170 180 190 200
ADQLVEAIKA KENGKVVVVG GGYIGLELGA ALRINNFDVS MVYPEPWCMP
210 220 230 240 250
RLFTPEIAAF YEGYYAQKGI TIIKGTVAVG FTVDTNGEVK EVKLKDGRVL
260 270 280 290 300
EADIVVVGVG ARPLTSLFKG QIVEEKGGIK TDEFFKTSVP DVYAVGDVAT
310 320 330 340 350
FPLKLYNELR RVEHVDHSRK SAEQAVKAIK ASEEGKAIEE YDYLPYFYSR
360 370 380 390 400
SFDLSWQFYG DNVGDAVLFG DNSPDSATHK FGSYWIKDGK VVGAFLESGS
410 420 430
PEENKAIAKV ARIQPSVESS DLLLKEGISF ASKV
Length:434
Mass (Da):47,416
Last modified:November 1, 1996 - v1
Checksum:i302DA61F8C3E4E23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D26392 mRNA Translation: BAA05408.1
PIRiJU0182
RefSeqiNP_001267683.1, NM_001280754.1

Genome annotation databases

GeneIDi101210277
KEGGicsv:101210277

Similar proteinsi

Entry informationi

Entry nameiMDARS_CUCSA
AccessioniPrimary (citable) accession number: Q42711
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2004
Last sequence update: November 1, 1996
Last modified: May 23, 2018
This is version 84 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

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