Reviewed, UniProtKB/Swiss-Prot Q42699 (METE_CATRO)

Names and origin

Protein names

Recommended name:
    5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
    EC=2.1.1.14
Alternative name(s):
    Vitamin-B12-independent methionine synthase isozyme
    Cobalamin-independent methionine synthase isozyme

Gene names

Name:

METE

Organism

Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)

Taxonomic identifier

4058 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthus

Protein attributes

Sequence length	765 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation.
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine.
Cofactor	Zinc By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1.
Subcellular location	Cytoplasm Potential.
Miscellaneous	Has an absolute requirement for a polyglutamylated folate as substrate.
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 765	765	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase		PRO_0000098697
Sites
	Metal binding	647	1	Zinc By similarity
	Metal binding	649	1	Zinc By similarity
	Metal binding	733	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42699-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 7398A1DC8AE960CE	FASTA	765	84,857
	10 20 30 40 50 60 MASHIVGYPR MGPKRELKFA LESFWDKKSS AEDLQKVAAD LRSSIWKQMA DAGIKYIPSN 70 80 90 100 110 120 TFSYYDQVLD TATMLGAVPP RYNFAGGEIG FDTYFSMARG NASVPAMEMT KWFDTNYHYI 130 140 150 160 170 180 VPELGPEVNF SYASHKAVNE YKEAKELGVD TVPVLVGPVT FLLLSKPAKG VEKTFPLLSL 190 200 210 220 230 240 LDKILPVYKE VIGELKAAGA SWIQFDEPTL VLDLESHQLE AFTKAYSELE STLSGLNVIV 250 260 270 280 290 300 ETYFADIPAE TYKILTALKG VTGFGFDLVR GAKTLDLIKG GFPSGKYLFA GVVDGRNIWA 310 320 330 340 350 360 NDLAASLSTL QSLEGIVGKD KLVVSTSCSL LHTAVDLVNE PKLDKEIKSW LAFAAQKVVE 370 380 390 400 410 420 VNALAKALAG EKDEAFFSEN AAAQASRKSS PRVTNQAVQK AAAALRGSDH RRATTVSARL 430 440 450 460 470 480 DAQQKKLNLP VLPTTTIGSF PQTLELRRVR REYKAKKISE DDYVKAIKEE ISKVVKLQEE 490 500 510 520 530 540 LDIDVLVHGE PERNDMVEYF GEQLSGFAFT ANGWVQSYGS RCVKPPIIYG DVSRPNPMTV 550 560 570 580 590 600 FWSQTAQSMT KRPMKGMLTG PVTILNWSFV RNDQPRFETC YQIALAIKDE VEDLEKAGIN 610 620 630 640 650 660 VIQIDEAALR EGLPLRKAEH AFYLDWAVHS FRITNLPLQD TTQIHTHMCY SNFNDIIHSI 670 680 690 700 710 720 IDMDADVMTI ENSRSSEKLL SVFREGVKYG AGIGPGVYDI HSPRIPSTEE IADRINKMLA 730 740 750 760 VLDTNILWVN PDCGLKTRKY AEVKPALENM VSAAKLIRTQ LASAK

References

[1]

"Vitamin-B12-independent methionine synthase from a higher plant (Catharanthus roseus). Molecular characterization, regulation, heterologous expression, and enzyme properties." Eichel J., Gonzalez J.C., Hotze M., Matthews R.G., Schroeder J. Eur. J. Biochem. 230:1053-1058(1995) [PubMed: 7601135] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X83499 mRNA. Translation: CAA58474.1.
PIR	S57636.
3D structure databases
SMR	Q42699. Positions 2-760.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.1.1.14. 20471.
Family and domain databases
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR002629. Methionine_synth. IPR006276. MeTrfase_B12_ind. [Graphical view]
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
ProDom	PD004692. Methionine_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01371. met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_CATRO

Accession

Primary (citable) accession number: Q42699

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)