Reviewed, UniProtKB/Swiss-Prot Q42698 (GGPPS_CATRO)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl pyrophosphate synthetase, chloroplastic
      Short name=GGPP synthetase
      Short name=GGPS
Including the following 3 domains:
    1- Recommended name:
            Dimethylallyltranstransferase
              EC=2.5.1.1
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10
    3- Recommended name:
            Farnesyltranstransferase
              EC=2.5.1.29

Gene names

Name:	GGPS1
Synonyms:	GGC1

Organism

Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)

Taxonomic identifier

4058 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate. Trans,trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Pathway	Isoprenoid biosynthesis; farnesyl-PP biosynthesis; farnesyl-PP from geranyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranyl-PP biosynthesis; geranyl-PP from dimethylallyl-PP and isopentenyl-PP: step 1/1. Isoprenoid biosynthesis; geranylgeranyl-PP biosynthesis; geranylgeranyl-PP from farnesyl-PP and isopentenyl-PP: step 1/1.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the FPP/GGPP synthetase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC farnesyltranstransferase activity Inferred from electronic annotation. Source: EC geranyltranstransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Chloroplast Potential
	Chain	41 – 357	317	Geranylgeranyl pyrophosphate synthetase, chloroplastic		PRO_0000016473

Sequences

	Sequence		Length	Mass (Da)
	Q42698-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 81C52FDEA1E06FA8	FASTA	357	38,787
	10 20 30 40 50 60 MRSNLCHPLK NQLPISFFLS GTIRKPIFSC SRLSISAIIT KEQTQEESES KSKKEVAFSS 70 80 90 100 110 120 SSSFDFKAYM IGKANSVNKA LEDAVLVREP LKIHESMRYS LLAGGKRVRP MLCIAACELF 130 140 150 160 170 180 GGTESVAMPS ACAVEMIHTM SLMHDDLPCM DNDDLRRGKP TNHKVFGEDV AVLAGDALLA 190 200 210 220 230 240 FAFEHIATAT KGVSSERIVR VVGELAKCIG SEGLVAGQVV DVCSEGIADV GLEHLEFIHI 250 260 270 280 290 300 HKTAALLEGS VVLGAIVGGA NDEQISKLRK FARCIGLLFQ VVDDILDVTK SSQELGKTAG 310 320 330 340 350 KDLVADKVTY PKLLGIDKSR EFAEKLNREA QEQLAEFDPE KAAPLIALAN YIAYRDN

References

[1]	"Nucleotide sequence of a Catharanthus roseus geranylgeranyl pyrophosphate synthase gene." Bantignies B., Liboz T., Ambid C. Plant Gene Register PGR95-119 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. G. Don c20.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X92893 Genomic DNA. Translation: CAA63486.1.
PIR	T09966.
3D structure databases
SMR	Q42698. Positions 68-352.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.1. 20471. 2.5.1.10. 20471. 2.5.1.29. 20471.
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGPPS_CATRO

Accession

Primary (citable) accession number: Q42698

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)