Q42698 (GGPPS_CATRO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Geranylgeranyl pyrophosphate synthase, chloroplastic
Short name=GGPP synthase
Short name=GGPS

Alternative name(s):
(2E,6E)-farnesyl diphosphate synthase
Dimethylallyltranstransferase
EC=2.5.1.1
Farnesyl diphosphate synthase
Farnesyltranstransferase
EC=2.5.1.29
Geranyltranstransferase
EC=2.5.1.10

Gene names

Name:	GGPS1
Synonyms:	GGC1

Organism

Catharanthus roseus (Madagascar periwinkle) (Vinca rosea)

Taxonomic identifier

4058 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Gentianales › Apocynaceae › Rauvolfioideae › Vinceae › Catharanthus

Protein attributes

Sequence length	357 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate. (2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1. Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1. Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the FPP/GGPP synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC farnesyltranstransferase activity Inferred from electronic annotation. Source: EC geranyltranstransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Chloroplast Potential
	Chain	41 – 357	317	Geranylgeranyl pyrophosphate synthase, chloroplastic		PRO_0000016473
Sites
	Metal binding	145	1	Magnesium 1 By similarity
	Metal binding	145	1	Magnesium 2 By similarity
	Metal binding	151	1	Magnesium 1 By similarity
	Metal binding	151	1	Magnesium 2 By similarity
	Metal binding	283	1	Magnesium 3 By similarity
	Binding site	106	1	Isopentenyl diphosphate By similarity
	Binding site	109	1	Isopentenyl diphosphate By similarity
	Binding site	138	1	Isopentenyl diphosphate By similarity
	Binding site	156	1	Dimethylallyl diphosphate By similarity
	Binding site	157	1	Isopentenyl diphosphate By similarity
	Binding site	242	1	Dimethylallyl diphosphate By similarity
	Binding site	243	1	Dimethylallyl diphosphate By similarity
	Binding site	280	1	Dimethylallyl diphosphate By similarity
	Binding site	297	1	Dimethylallyl diphosphate By similarity
	Binding site	307	1	Dimethylallyl diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42698 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 81C52FDEA1E06FA8	FASTA	357	38,787
	10 20 30 40 50 60 MRSNLCHPLK NQLPISFFLS GTIRKPIFSC SRLSISAIIT KEQTQEESES KSKKEVAFSS 70 80 90 100 110 120 SSSFDFKAYM IGKANSVNKA LEDAVLVREP LKIHESMRYS LLAGGKRVRP MLCIAACELF 130 140 150 160 170 180 GGTESVAMPS ACAVEMIHTM SLMHDDLPCM DNDDLRRGKP TNHKVFGEDV AVLAGDALLA 190 200 210 220 230 240 FAFEHIATAT KGVSSERIVR VVGELAKCIG SEGLVAGQVV DVCSEGIADV GLEHLEFIHI 250 260 270 280 290 300 HKTAALLEGS VVLGAIVGGA NDEQISKLRK FARCIGLLFQ VVDDILDVTK SSQELGKTAG 310 320 330 340 350 KDLVADKVTY PKLLGIDKSR EFAEKLNREA QEQLAEFDPE KAAPLIALAN YIAYRDN

References

[1]	"Nucleotide sequence of a Catharanthus roseus geranylgeranyl pyrophosphate synthase gene." Bantignies B., Liboz T., Ambid C. Plant Gene Register PGR95-119 Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. G. Don c20.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92893 Genomic DNA. Translation: CAA63486.1.
PIR	T09966.
3D structure databases
ProteinModelPortal	Q42698.
SMR	Q42698. Positions 68-352.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
PROSITE	PS00723. POLYPRENYL_SYNTHASE_1. 1 hit. PS00444. POLYPRENYL_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGPPS_CATRO

Accession

Primary (citable) accession number: Q42698

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	November 1, 1996
Last modified:	September 21, 2011
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program