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Q42690

- ALFC_CHLRE

UniProt

Q42690 - ALFC_CHLRE

Protein

Fructose-bisphosphate aldolase 1, chloroplastic

Gene

ALDCHL

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 2 (22 Feb 2012)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei74 – 741SubstrateBy similarity
    Binding sitei164 – 1641SubstrateBy similarity
    Active sitei204 – 2041Proton acceptorBy similarity
    Active sitei246 – 2461Schiff-base intermediate with dihydroxyacetone-PBy similarity
    Sitei377 – 3771Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

    GO - Molecular functioni

    1. fructose-bisphosphate aldolase activity Source: UniProtKB-EC

    GO - Biological processi

    1. glycolytic process Source: UniProtKB-UniPathway
    2. response to abscisic acid Source: EnsemblPlants/Gramene
    3. response to cadmium ion Source: EnsemblPlants/Gramene
    4. response to oxidative stress Source: EnsemblPlants/Gramene

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    Schiff base

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00183.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Fructose-bisphosphate aldolase 1, chloroplastic (EC:4.1.2.13)
    Gene namesi
    Name:ALDCHL
    ORF Names:CHLREDRAFT_24459
    OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
    Taxonomic identifieri3055 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
    ProteomesiUP000006906: Unassembled WGS sequence

    Subcellular locationi

    GO - Cellular componenti

    1. apoplast Source: EnsemblPlants/Gramene
    2. chloroplast envelope Source: EnsemblPlants/Gramene
    3. chloroplast stroma Source: EnsemblPlants/Gramene
    4. chloroplast thylakoid Source: EnsemblPlants/Gramene
    5. cytosolic ribosome Source: EnsemblPlants/Gramene
    6. membrane Source: EnsemblPlants/Gramene
    7. mitochondrion Source: EnsemblPlants/Gramene
    8. thylakoid lumen Source: EnsemblPlants/Gramene

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 377Fructose-bisphosphate aldolase 1, chloroplasticPRO_0000001111
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PRIDEiQ42690.
    ProMEXiQ42690.

    Interactioni

    Protein-protein interaction databases

    STRINGi3055.JGI24459.

    Structurei

    3D structure databases

    ProteinModelPortaliQ42690.
    SMRiQ42690. Positions 28-363.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG3588.
    KOiK01623.
    OMAiVEPEIML.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view]
    PfamiPF00274. Glycolytic. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q42690-1 [UniParc]FASTAAdd to Basket

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    MALMMKSSAS LKAVSAGRSR RAVVVRAGKY DEELIKTAGT VASKGRGILA    50
    MDESNATCGK RLDSIGVENT EENRRAYREL LVTAPGLGQY ISGAILFEET 100
    LYQSTASGKK FVDVMKEQNI VPGIKVDKGL VPLSNTNGES WCMGLDGLDK 150
    RCAEYYKAGA RFAKWRSVVS IPHGPSIIAA RDCAYGLARY AAIAQNAGLV 200
    PIVEPEVLLD GEHDIDRCLE VQEAIWAETF KYMADNKVMF EGILLKPAMV 250
    TPGADCKNKA GPAKVAEYTL KMLRRRVPPA VPGIMFLSGG QSELESTLNL 300
    NAMNQSPNPW HVSFSYARAL QNTVLKTWQG KPENVQAAQA ALLKRAKANS 350
    DAQQGKYDAT TEGKEAAQGM YEKGYVY 377
    Length:377
    Mass (Da):40,985
    Last modified:February 22, 2012 - v2
    Checksum:i76B5BED0C422E77C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti16 – 2712AGRSR…VVVRA → LAALAAPSLCAP in AAC60574. (PubMed:8053679)CuratedAdd
    BLAST
    Sequence conflicti16 – 2712AGRSR…VVVRA → LAALAAPSLCAP in CAA49590. (PubMed:8053679)CuratedAdd
    BLAST
    Sequence conflicti133 – 1397LSNTNGE → CPTPTM in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti133 – 1397LSNTNGE → CPTPTM in CAA49590. (PubMed:8053679)Curated
    Sequence conflicti148 – 1525LDKRC → WTSA in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti148 – 1525LDKRC → WTSA in CAA49590. (PubMed:8053679)Curated
    Sequence conflicti178 – 1836IAARDC → MLPRL in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti178 – 1836IAARDC → MLPRL in CAA49590. (PubMed:8053679)Curated
    Sequence conflicti240 – 2412FE → LQ in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti240 – 2412FE → LQ in CAA49590. (PubMed:8053679)Curated
    Sequence conflicti274 – 2807RRRVPPA → AARAPP in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti274 – 2807RRRVPPA → AARAPP in CAA49590. (PubMed:8053679)Curated
    Sequence conflicti321 – 3211Q → T in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti321 – 3211Q → T in CAA49590. (PubMed:8053679)Curated
    Sequence conflicti330 – 3345GKPEN → ASPRT in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti330 – 3345GKPEN → ASPRT in CAA49590. (PubMed:8053679)Curated
    Sequence conflicti338 – 3469AQAALLKRA → PRLAAQAR in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti338 – 3469AQAALLKRA → PRLAAQAR in CAA49590. (PubMed:8053679)Curated
    Sequence conflicti374 – 3741G → GKG in AAC60574. (PubMed:8053679)Curated
    Sequence conflicti374 – 3741G → GKG in CAA49590. (PubMed:8053679)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69969 mRNA. Translation: CAA49590.1.
    S72951 Genomic DNA. Translation: AAC60574.1.
    X85495 Genomic DNA. No translation available.
    DS496165 Genomic DNA. Translation: EDO97897.1.
    PIRiS48639.
    RefSeqiXP_001700659.1. XM_001700607.1.
    UniGeneiCre.9890.

    Genome annotation databases

    EnsemblPlantsiEDO97897; EDO97897; CHLREDRAFT_24459.
    GeneIDi5726208.
    KEGGicre:CHLREDRAFT_24459.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X69969 mRNA. Translation: CAA49590.1 .
    S72951 Genomic DNA. Translation: AAC60574.1 .
    X85495 Genomic DNA. No translation available.
    DS496165 Genomic DNA. Translation: EDO97897.1 .
    PIRi S48639.
    RefSeqi XP_001700659.1. XM_001700607.1.
    UniGenei Cre.9890.

    3D structure databases

    ProteinModelPortali Q42690.
    SMRi Q42690. Positions 28-363.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3055.JGI24459.

    Proteomic databases

    PRIDEi Q42690.
    ProMEXi Q42690.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi EDO97897 ; EDO97897 ; CHLREDRAFT_24459 .
    GeneIDi 5726208.
    KEGGi cre:CHLREDRAFT_24459.

    Phylogenomic databases

    eggNOGi COG3588.
    KOi K01623.
    OMAi VEPEIML.

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00183 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR000741. FBA_I.
    [Graphical view ]
    Pfami PF00274. Glycolytic. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression and sequence of the only detectable aldolase in Chlamydomonas reinhardtii."
      Schnarrenberger C., Pelzer-Reith B., Yatsuki H., Freund S., Jacobshagen S., Hori K.
      Arch. Biochem. Biophys. 313:173-178(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    2. "The plastid aldolase gene from Chlamydomonas reinhardtii: intron/exon organization, evolution, and promoter structure."
      Pelzer-Reith B., Freund S., Schnarrenberger C., Yatsuki H., Hori K.
      Mol. Gen. Genet. 248:481-486(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The Chlamydomonas genome reveals the evolution of key animal and plant functions."
      Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J., Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L., Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H., Kapitonov V.V., Ren Q., Ferris P.
      , Lindquist E., Shapiro H., Lucas S.M., Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L., Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H., Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M., Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A., Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L., Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C., Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J., Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L., Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L., Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C., Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J., Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P., Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R., Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P., Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y., Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L., Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.
      Science 318:245-250(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: CC-503.

    Entry informationi

    Entry nameiALFC_CHLRE
    AccessioniPrimary (citable) accession number: Q42690
    Secondary accession number(s): A8JE10, Q36725
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: February 22, 2012
    Last modified: October 1, 2014
    This is version 78 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3