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Protein

Fructose-bisphosphate aldolase 1, chloroplastic

Gene

ALDCHL

Organism
Chlamydomonas reinhardtii (Chlamydomonas smithii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Pathway:iglycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (PGI1), Glucose-6-phosphate isomerase (GPI)
  3. no protein annotated in this organism
  4. Fructose-bisphosphate aldolase 1, chloroplastic (ALDCHL), Fructose-bisphosphate aldolase (FBA2), Fructose-bisphosphate aldolase (FBA1), Fructose-bisphosphate aldolase (FBA4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei74 – 741SubstrateBy similarity
Binding sitei164 – 1641SubstrateBy similarity
Active sitei204 – 2041Proton acceptorBy similarity
Active sitei246 – 2461Schiff-base intermediate with dihydroxyacetone-PBy similarity
Sitei377 – 3771Necessary for preference for fructose 1,6-bisphosphate over fructose 1-phosphateBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase 1, chloroplastic (EC:4.1.2.13)
Gene namesi
Name:ALDCHL
ORF Names:CHLREDRAFT_24459
OrganismiChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifieri3055 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas
ProteomesiUP000006906 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 377Fructose-bisphosphate aldolase 1, chloroplasticPRO_0000001111
Transit peptidei1 – ?ChloroplastSequence Analysis

Proteomic databases

PRIDEiQ42690.
ProMEXiQ42690.

Interactioni

Protein-protein interaction databases

STRINGi3055.EDO97897.

Structurei

3D structure databases

ProteinModelPortaliQ42690.
SMRiQ42690. Positions 28-363.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG3588.
InParanoidiQ42690.
KOiK01623.
OMAiTWQGKPE.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALMMKSSAS LKAVSAGRSR RAVVVRAGKY DEELIKTAGT VASKGRGILA
60 70 80 90 100
MDESNATCGK RLDSIGVENT EENRRAYREL LVTAPGLGQY ISGAILFEET
110 120 130 140 150
LYQSTASGKK FVDVMKEQNI VPGIKVDKGL VPLSNTNGES WCMGLDGLDK
160 170 180 190 200
RCAEYYKAGA RFAKWRSVVS IPHGPSIIAA RDCAYGLARY AAIAQNAGLV
210 220 230 240 250
PIVEPEVLLD GEHDIDRCLE VQEAIWAETF KYMADNKVMF EGILLKPAMV
260 270 280 290 300
TPGADCKNKA GPAKVAEYTL KMLRRRVPPA VPGIMFLSGG QSELESTLNL
310 320 330 340 350
NAMNQSPNPW HVSFSYARAL QNTVLKTWQG KPENVQAAQA ALLKRAKANS
360 370
DAQQGKYDAT TEGKEAAQGM YEKGYVY
Length:377
Mass (Da):40,985
Last modified:February 22, 2012 - v2
Checksum:i76B5BED0C422E77C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti16 – 2712AGRSR…VVVRA → LAALAAPSLCAP in AAC60574 (PubMed:8053679).CuratedAdd
BLAST
Sequence conflicti16 – 2712AGRSR…VVVRA → LAALAAPSLCAP in CAA49590 (PubMed:8053679).CuratedAdd
BLAST
Sequence conflicti133 – 1397LSNTNGE → CPTPTM in AAC60574 (PubMed:8053679).Curated
Sequence conflicti133 – 1397LSNTNGE → CPTPTM in CAA49590 (PubMed:8053679).Curated
Sequence conflicti148 – 1525LDKRC → WTSA in AAC60574 (PubMed:8053679).Curated
Sequence conflicti148 – 1525LDKRC → WTSA in CAA49590 (PubMed:8053679).Curated
Sequence conflicti178 – 1836IAARDC → MLPRL in AAC60574 (PubMed:8053679).Curated
Sequence conflicti178 – 1836IAARDC → MLPRL in CAA49590 (PubMed:8053679).Curated
Sequence conflicti240 – 2412FE → LQ in AAC60574 (PubMed:8053679).Curated
Sequence conflicti240 – 2412FE → LQ in CAA49590 (PubMed:8053679).Curated
Sequence conflicti274 – 2807RRRVPPA → AARAPP in AAC60574 (PubMed:8053679).Curated
Sequence conflicti274 – 2807RRRVPPA → AARAPP in CAA49590 (PubMed:8053679).Curated
Sequence conflicti321 – 3211Q → T in AAC60574 (PubMed:8053679).Curated
Sequence conflicti321 – 3211Q → T in CAA49590 (PubMed:8053679).Curated
Sequence conflicti330 – 3345GKPEN → ASPRT in AAC60574 (PubMed:8053679).Curated
Sequence conflicti330 – 3345GKPEN → ASPRT in CAA49590 (PubMed:8053679).Curated
Sequence conflicti338 – 3469AQAALLKRA → PRLAAQAR in AAC60574 (PubMed:8053679).Curated
Sequence conflicti338 – 3469AQAALLKRA → PRLAAQAR in CAA49590 (PubMed:8053679).Curated
Sequence conflicti374 – 3741G → GKG in AAC60574 (PubMed:8053679).Curated
Sequence conflicti374 – 3741G → GKG in CAA49590 (PubMed:8053679).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69969 mRNA. Translation: CAA49590.1.
S72951 Genomic DNA. Translation: AAC60574.1.
X85495 Genomic DNA. No translation available.
DS496165 Genomic DNA. Translation: EDO97897.1.
PIRiS48639.
RefSeqiXP_001700659.1. XM_001700607.1.
UniGeneiCre.9890.

Genome annotation databases

EnsemblPlantsiEDO97897; EDO97897; CHLREDRAFT_24459.
GeneIDi5726208.
KEGGicre:CHLREDRAFT_24459.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X69969 mRNA. Translation: CAA49590.1.
S72951 Genomic DNA. Translation: AAC60574.1.
X85495 Genomic DNA. No translation available.
DS496165 Genomic DNA. Translation: EDO97897.1.
PIRiS48639.
RefSeqiXP_001700659.1. XM_001700607.1.
UniGeneiCre.9890.

3D structure databases

ProteinModelPortaliQ42690.
SMRiQ42690. Positions 28-363.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3055.EDO97897.

Proteomic databases

PRIDEiQ42690.
ProMEXiQ42690.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiEDO97897; EDO97897; CHLREDRAFT_24459.
GeneIDi5726208.
KEGGicre:CHLREDRAFT_24459.

Phylogenomic databases

eggNOGiCOG3588.
InParanoidiQ42690.
KOiK01623.
OMAiTWQGKPE.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR029769. FBA_euk-type.
IPR000741. FBA_I.
[Graphical view]
PANTHERiPTHR11627. PTHR11627. 1 hit.
PfamiPF00274. Glycolytic. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Expression and sequence of the only detectable aldolase in Chlamydomonas reinhardtii."
    Schnarrenberger C., Pelzer-Reith B., Yatsuki H., Freund S., Jacobshagen S., Hori K.
    Arch. Biochem. Biophys. 313:173-178(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
  2. "The plastid aldolase gene from Chlamydomonas reinhardtii: intron/exon organization, evolution, and promoter structure."
    Pelzer-Reith B., Freund S., Schnarrenberger C., Yatsuki H., Hori K.
    Mol. Gen. Genet. 248:481-486(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The Chlamydomonas genome reveals the evolution of key animal and plant functions."
    Merchant S.S., Prochnik S.E., Vallon O., Harris E.H., Karpowicz S.J., Witman G.B., Terry A., Salamov A., Fritz-Laylin L.K., Marechal-Drouard L., Marshall W.F., Qu L.H., Nelson D.R., Sanderfoot A.A., Spalding M.H., Kapitonov V.V., Ren Q., Ferris P.
    , Lindquist E., Shapiro H., Lucas S.M., Grimwood J., Schmutz J., Cardol P., Cerutti H., Chanfreau G., Chen C.L., Cognat V., Croft M.T., Dent R., Dutcher S., Fernandez E., Fukuzawa H., Gonzalez-Ballester D., Gonzalez-Halphen D., Hallmann A., Hanikenne M., Hippler M., Inwood W., Jabbari K., Kalanon M., Kuras R., Lefebvre P.A., Lemaire S.D., Lobanov A.V., Lohr M., Manuell A., Meier I., Mets L., Mittag M., Mittelmeier T., Moroney J.V., Moseley J., Napoli C., Nedelcu A.M., Niyogi K., Novoselov S.V., Paulsen I.T., Pazour G.J., Purton S., Ral J.P., Riano-Pachon D.M., Riekhof W., Rymarquis L., Schroda M., Stern D., Umen J., Willows R., Wilson N., Zimmer S.L., Allmer J., Balk J., Bisova K., Chen C.J., Elias M., Gendler K., Hauser C., Lamb M.R., Ledford H., Long J.C., Minagawa J., Page M.D., Pan J., Pootakham W., Roje S., Rose A., Stahlberg E., Terauchi A.M., Yang P., Ball S., Bowler C., Dieckmann C.L., Gladyshev V.N., Green P., Jorgensen R., Mayfield S., Mueller-Roeber B., Rajamani S., Sayre R.T., Brokstein P., Dubchak I., Goodstein D., Hornick L., Huang Y.W., Jhaveri J., Luo Y., Martinez D., Ngau W.C., Otillar B., Poliakov A., Porter A., Szajkowski L., Werner G., Zhou K., Grigoriev I.V., Rokhsar D.S., Grossman A.R.
    Science 318:245-250(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC-503.

Entry informationi

Entry nameiALFC_CHLRE
AccessioniPrimary (citable) accession number: Q42690
Secondary accession number(s): A8JE10, Q36725
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: February 22, 2012
Last modified: July 22, 2015
This is version 83 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.