Reviewed, UniProtKB/Swiss-Prot Q42689 (GLNA2_CHLRE)

Names and origin

Protein names

Recommended name:
    Glutamine synthetase, chloroplastic
    EC=6.3.1.2
Alternative name(s):
    GS2
    Glutamate--ammonia ligase

Gene names

Name:

GLN2

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	380 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 380		Glutamine synthetase, chloroplastic		PRO_0000011176

Sequences

	Sequence		Length	Mass (Da)
	Q42689-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 7BD53794374E6A8C	FASTA	380	41,285
	10 20 30 40 50 60 MAFALRGVTA KASGRTAGAR SSGRTLTVRV QAYGMAAEYI WADGNEGKPE KGMIFNEMRS 70 80 90 100 110 120 KTKCFEAPLG LDASEYPDWS FDGSSTGQAE GNNSDCILRP VRVVTDPIRG APHVLVMCEV 130 140 150 160 170 180 FAPDGKPHST NTRAKLREII DDKVTAEDCW YGFEQEYTML AKTSGHIYGW PAGGFPAPQG 190 200 210 220 230 240 PFYCGVGAES AFGRPLAEAH MEACMKAGLV ISGINAEVMP GQWEYQIGPV GPLALGDEVM 250 260 270 280 290 300 LSRWLLHRLG EDFGIVSTFN PKPVRTGDWN GTGAHTNFST KGMRVPGGMK VIEEAVEKLS 310 320 330 340 350 360 KTHIEHITQY GIGNEARLTG KHETCDINTF KHGVADRGSS IRIPLPVMLK GYGYLEDRRP 370 380 AANVDPYTVA RLLIKTVLKG

References

[1]	"Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii." Chen Q., Silflow C.D. Plant Physiol. 112:987-996(1996) [PubMed: 8938407] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: A55.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U46208 mRNA. Translation: AAB01818.1.
PIR	T08090.
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q42689.
Enzyme and pathway databases
BRENDA	6.3.1.2. 144.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
ProDom	PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA2_CHLRE

Accession

Primary (citable) accession number: Q42689

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)