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Q42688 (GLNA1_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine synthetase cytosolic isozyme

EC=6.3.1.2
Alternative name(s):
GS1
Glutamate--ammonia ligase
Gene names
Name:GLN1
OrganismChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Protein attributes

Sequence length382 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.

Subunit structure

Homooctamer By similarity.

Subcellular location

Cytoplasm.

Miscellaneous

Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).

Sequence similarities

Belongs to the glutamine synthetase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_processglutamine biosynthetic process

Inferred from electronic annotation. Source: InterPro

response to cadmium ion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to salt stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Cellular_componentapoplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast envelope

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast stroma

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast thylakoid membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cytosolic ribosome

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glutamate-ammonia ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 382382Glutamine synthetase cytosolic isozyme
PRO_0000153169

Sequences

Sequence LengthMass (Da)Tools
Q42688 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: CC806E221EE461F5

FASTA38242,146
        10         20         30         40         50         60 
MAAGSVGVFA TDEKIGSLLD QSITRHFLST VTDQQGKICA EYVWIGGSMH DVRSKSRTLS 

        70         80         90        100        110        120 
TIPTKPEDLP HWNYDGSSTG QAPGHDSEVY LIPRSIFKDP FRGGDNILVM CDCYEPPKVN 

       130        140        150        160        170        180 
PDGTLAAPKP IPTNTRFACA EVMEKAKKEE PWFGIEQEYT LLNAITKWPL GWPKGGYPAP 

       190        200        210        220        230        240 
QGPYYCSAGA GVAIGRDVAE VHYRLCLAAG VNISGVNAEV LPSQWEYQVG PCEGITMGDH 

       250        260        270        280        290        300 
MWMSRYIMYR VCEMFNVEVS FDPKPIPGDW NGSGGHTNYS TKATRTAPDG WKVIQEHCAK 

       310        320        330        340        350        360 
LEARHAVHIA AYGEGNERRL TGKHETSSMS DFSWGVANRG CSIRVGRMVP VEKSGYYEDR 

       370        380 
RPASNLDAYV VTRLIVETTI LL 

« Hide

References

[1]"Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii."
Chen Q., Silflow C.D.
Plant Physiol. 112:987-996(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: A55.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U46207 mRNA. Translation: AAB01817.1.
PIRT08088.
RefSeqXP_001699902.1. XM_001699850.1.
UniGeneCre.13251.

3D structure databases

ProteinModelPortalQ42688.
SMRQ42688. Positions 31-381.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ42688.
ProMEXQ42688.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5725229.
KEGGcre:CHLREDRAFT_133971.

Phylogenomic databases

eggNOGCOG0174.
KOK01915.
ProtClustDBPLN02284.

Family and domain databases

Gene3D3.30.590.10. 1 hit.
InterProIPR008147. Gln_synt_beta.
IPR014746. Gln_synth/guanido_kin_cat_dom.
IPR008146. Gln_synth_cat_dom.
IPR027303. Gln_synth_gly_rich_site.
IPR027302. Gln_synth_N_conserv_site.
[Graphical view]
PfamPF00120. Gln-synt_C. 1 hit.
PF03951. Gln-synt_N. 1 hit.
[Graphical view]
SUPFAMSSF54368. SSF54368. 1 hit.
PROSITEPS00180. GLNA_1. 1 hit.
PS00181. GLNA_ATP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLNA1_CHLRE
AccessionPrimary (citable) accession number: Q42688
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: March 19, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families