Reviewed, UniProtKB/Swiss-Prot Q42688 (GLNA1_CHLRE)

Names and origin

Protein names

Recommended name:
    Glutamine synthetase cytosolic isozyme
    EC=6.3.1.2
Alternative name(s):
    GS1
    Glutamate--ammonia ligase

Gene names

Name:

GLN1

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	382 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + L-glutamate + NH3 = ADP + phosphate + L-glutamine.
Subunit structure	Homooctamer By similarity.
Subcellular location	Cytoplasm.
Miscellaneous	Irreversibly inhibited by the herbicide L-phosphinothricin (PPT).
Sequence similarities	Belongs to the glutamine synthetase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	glutamine biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-ammonia ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 382	382	Glutamine synthetase cytosolic isozyme		PRO_0000153169

Sequences

	Sequence		Length	Mass (Da)
	Q42688-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: CC806E221EE461F5	FASTA	382	42,146
	10 20 30 40 50 60 MAAGSVGVFA TDEKIGSLLD QSITRHFLST VTDQQGKICA EYVWIGGSMH DVRSKSRTLS 70 80 90 100 110 120 TIPTKPEDLP HWNYDGSSTG QAPGHDSEVY LIPRSIFKDP FRGGDNILVM CDCYEPPKVN 130 140 150 160 170 180 PDGTLAAPKP IPTNTRFACA EVMEKAKKEE PWFGIEQEYT LLNAITKWPL GWPKGGYPAP 190 200 210 220 230 240 QGPYYCSAGA GVAIGRDVAE VHYRLCLAAG VNISGVNAEV LPSQWEYQVG PCEGITMGDH 250 260 270 280 290 300 MWMSRYIMYR VCEMFNVEVS FDPKPIPGDW NGSGGHTNYS TKATRTAPDG WKVIQEHCAK 310 320 330 340 350 360 LEARHAVHIA AYGEGNERRL TGKHETSSMS DFSWGVANRG CSIRVGRMVP VEKSGYYEDR 370 380 RPASNLDAYV VTRLIVETTI LL

References

[1]	"Isolation and characterization of glutamine synthetase genes in Chlamydomonas reinhardtii." Chen Q., Silflow C.D. Plant Physiol. 112:987-996(1996) [PubMed: 8938407] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: A55.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U46207 mRNA. Translation: AAB01817.1.
PIR	T08088.
RefSeq	XP_001699902.1.
UniGene	Cre.13251
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q42688.
Genome annotation databases
GeneID	5725229.
KEGG	cre:CHLREDRAFT_133971.
Enzyme and pathway databases
BRENDA	6.3.1.2. 144.
Family and domain databases
InterPro	IPR008147. Gln_synt_beta. IPR014746. Gln_synth/guanido_kin_cat. IPR008146. Gln_synth_cat. [Graphical view]
Gene3D	G3DSA:3.30.590.10. ATP-gua_Ptrans. 1 hit.
Pfam	PF00120. Gln-synt_C. 1 hit. PF03951. Gln-synt_N. 1 hit. [Graphical view]
ProDom	PD001057. Gln_synt_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00180. GLNA_1. 1 hit. PS00181. GLNA_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNA1_CHLRE

Accession

Primary (citable) accession number: Q42688

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)