ID   MDHM_CHLRE              Reviewed;         373 AA.
AC   Q42686;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   16-JUN-2009, entry version 63.
DE   RecName: Full=Malate dehydrogenase, mitochondrial;
DE            EC=1.1.1.37;
DE   Flags: Precursor;
OS   Chlamydomonas reinhardtii.
OC   Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae;
OC   Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Krishnasamy S., Chang T.E., Makaroff C.A., Wang W.Y.;
RL   Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
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DR   EMBL; U40212; AAA84971.1; -; Genomic_DNA.
DR   PIR; T08077; T08077.
DR   RefSeq; XP_001703167.1; -.
DR   UniGene; Cre.14161; -.
DR   HSSP; P00346; 1MLD.
DR   PRIDE; Q42686; -.
DR   GeneID; 5728707; -.
DR   KEGG; cre:CHLREDRAFT_60444; -.
DR   BRENDA; 1.1.1.37; 144.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005488; F:binding; IEA:InterPro.
DR   GO; GO:0030060; F:L-malate dehydrogenase activity; IEA:EC.
DR   GO; GO:0006096; P:glycolysis; IEA:InterPro.
DR   GO; GO:0006108; P:malate metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR001557; L-lactate/malate_DH.
DR   InterPro; IPR001236; Lactate/malate_DH.
DR   InterPro; IPR015955; Lactate_DH/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DH_AS.
DR   InterPro; IPR010097; Malate_DH_NAD-dep_euk_g_bac.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11540:SF1; MDH_euk_g_bac; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   TIGRFAMs; TIGR01772; MDH_euk_gproteo; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   3: Inferred from homology;
KW   Mitochondrion; NAD; Oxidoreductase; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    373       Malate dehydrogenase, mitochondrial.
FT                                /FTId=PRO_0000018624.
FT   NP_BIND      69     75       NAD (By similarity).
FT   NP_BIND     177    179       NAD (By similarity).
FT   ACT_SITE    237    237       Proton acceptor (By similarity).
FT   BINDING      95     95       NAD (By similarity).
FT   BINDING     141    141       Substrate (By similarity).
FT   BINDING     147    147       Substrate (By similarity).
FT   BINDING     154    154       NAD (By similarity).
FT   BINDING     179    179       Substrate (By similarity).
FT   BINDING     213    213       Substrate (By similarity).
FT   BINDING     288    288       NAD (By similarity).
SQ   SEQUENCE   373 AA;  38510 MW;  0FCFEBFC58FD19BD CRC64;
     MSLQSSIRAD SNCTLPNNPV CVLLPVDFIV AAMASSTSSA MAKWAAQAAR GFAAAAPSSG
     KGRKVAVLGA AGGIGQPLSM LMKMNSQVSS LSLYDIAGTP GVAADVSHIN TKAQVKGFDK
     DGLAEALRGC DLVIIPAGVP RKPGMTRDDL FKINAGIVRD LVTAVGQHCP GAVLNIISNP
     VNSTVPIAAE QLKKMGVYDK RKVMGVTTLD VVRAKTFYAE KNGLDVASVD VPVVGGHAGV
     TILPLFSQAT PKATMSAEVL DALTKRTQDG GTEVVQAKAG KGSATLSMAY AAALFADSCL
     RGLNGAPVVE CTYVESTVTD APYFASKVKL STEGVDKIHD LGPLSDYEKA GLKAMMPELL
     ASIEKGVQFV KGA
//