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Reviewed, UniProtKB/Swiss-Prot Q42686 (MDHM_CHLRE)

Last modified October 13, 2009. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Malate dehydrogenase, mitochondrial
    EC=1.1.1.37
OrganismChlamydomonas reinhardtii
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

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Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

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Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological processglycolysis

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

oxidation reduction

Inferred from electronic annotation. Source: UniProtKB-KW

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: EC

binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 373Malate dehydrogenase, mitochondrialPRO_0000018624

Regions

Nucleotide binding69 – 757NAD By similarity
Nucleotide binding177 – 1793NAD By similarity

Sites

Active site2371Proton acceptor By similarity
Binding site951NAD By similarity
Binding site1411Substrate By similarity
Binding site1471Substrate By similarity
Binding site1541NAD By similarity
Binding site1791Substrate By similarity
Binding site2131Substrate By similarity
Binding site2881NAD By similarity

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Sequences

Sequence LengthMass (Da)Tools
Q42686-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 0FCFEBFC58FD19BD

FASTA37338,510
        10         20         30         40         50         60 
MSLQSSIRAD SNCTLPNNPV CVLLPVDFIV AAMASSTSSA MAKWAAQAAR GFAAAAPSSG 

        70         80         90        100        110        120 
KGRKVAVLGA AGGIGQPLSM LMKMNSQVSS LSLYDIAGTP GVAADVSHIN TKAQVKGFDK 

       130        140        150        160        170        180 
DGLAEALRGC DLVIIPAGVP RKPGMTRDDL FKINAGIVRD LVTAVGQHCP GAVLNIISNP 

       190        200        210        220        230        240 
VNSTVPIAAE QLKKMGVYDK RKVMGVTTLD VVRAKTFYAE KNGLDVASVD VPVVGGHAGV 

       250        260        270        280        290        300 
TILPLFSQAT PKATMSAEVL DALTKRTQDG GTEVVQAKAG KGSATLSMAY AAALFADSCL 

       310        320        330        340        350        360 
RGLNGAPVVE CTYVESTVTD APYFASKVKL STEGVDKIHD LGPLSDYEKA GLKAMMPELL 

       370 
ASIEKGVQFV KGA

« Hide

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References

[1]Krishnasamy S., Chang T.E., Makaroff C.A., Wang W.Y.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

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Cross-references

Sequence databases

U40212 Genomic DNA. Translation: AAA84971.1.
PIRT08077.
RefSeqXP_001703167.1.
UniGeneCre.14161

3D structure databases

HSSPHSSP built from PDB template 1MLD based on UniProtKB P00346.
ModBaseSearch...

Proteomic databases

PRIDEQ42686.

Genome annotation databases

GeneID5728707.
KEGGcre:CHLREDRAFT_60444.

Enzyme and pathway databases

BRENDA1.1.1.37. 144.

Family and domain databases

InterProIPR001557. L-lactate/malate_DH.
IPR001236. Lactate/malate_DH.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_NAD-dep_euk/g-bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
Gene3DG3DSA:3.90.110.10. lact_mal_DH. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHERPTHR11540:SF1. MDH_euk_g_bac. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameMDHM_CHLRE
AccessionPrimary (citable) accession number: Q42686
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 13, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectPPAP (Plant Proteome Annotation Project)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents