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Q42686 (MDHM_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase, mitochondrial

EC=1.1.1.37
OrganismChlamydomonas reinhardtii (Chlamydomonas smithii)
Taxonomic identifier3055 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion matrix.

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 1 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandNAD
   Molecular functionOxidoreductase
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response to bacterium

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

malate metabolic process

Inferred from electronic annotation. Source: InterPro

response to cadmium ion

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to cold

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

response to salt stress

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentapoplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

cell wall

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

chloroplast

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

membrane

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

mitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-EC

copper ion binding

Inferred from electronic annotation. Source: EnsemblPlants/Gramene

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Mitochondrion Potential
Chain? – 373Malate dehydrogenase, mitochondrialPRO_0000018624

Regions

Nucleotide binding69 – 757NAD By similarity
Nucleotide binding177 – 1793NAD By similarity

Sites

Active site2371Proton acceptor By similarity
Binding site951NAD By similarity
Binding site1411Substrate By similarity
Binding site1471Substrate By similarity
Binding site1541NAD By similarity
Binding site1791Substrate By similarity
Binding site2131Substrate By similarity
Binding site2881NAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q42686 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 0FCFEBFC58FD19BD

FASTA37338,510
        10         20         30         40         50         60 
MSLQSSIRAD SNCTLPNNPV CVLLPVDFIV AAMASSTSSA MAKWAAQAAR GFAAAAPSSG 

        70         80         90        100        110        120 
KGRKVAVLGA AGGIGQPLSM LMKMNSQVSS LSLYDIAGTP GVAADVSHIN TKAQVKGFDK 

       130        140        150        160        170        180 
DGLAEALRGC DLVIIPAGVP RKPGMTRDDL FKINAGIVRD LVTAVGQHCP GAVLNIISNP 

       190        200        210        220        230        240 
VNSTVPIAAE QLKKMGVYDK RKVMGVTTLD VVRAKTFYAE KNGLDVASVD VPVVGGHAGV 

       250        260        270        280        290        300 
TILPLFSQAT PKATMSAEVL DALTKRTQDG GTEVVQAKAG KGSATLSMAY AAALFADSCL 

       310        320        330        340        350        360 
RGLNGAPVVE CTYVESTVTD APYFASKVKL STEGVDKIHD LGPLSDYEKA GLKAMMPELL 

       370 
ASIEKGVQFV KGA 

« Hide

References

[1]Krishnasamy S., Chang T.E., Makaroff C.A., Wang W.Y.
Submitted (JAN-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U40212 Genomic DNA. Translation: AAA84971.1.
PIRT08077.
RefSeqXP_001703167.1. XM_001703115.1.
UniGeneCre.14161.

3D structure databases

ProteinModelPortalQ42686.
SMRQ42686. Positions 62-371.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEQ42686.
ProMEXQ42686.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5728707.
KEGGcre:CHLREDRAFT_60444.

Phylogenomic databases

eggNOGCOG0039.
KOK00026.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010097. Malate_DH_type1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01772. MDH_euk_gproteo. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMDHM_CHLRE
AccessionPrimary (citable) accession number: Q42686
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families