ID   HEM2_CHLRE              Reviewed;         390 AA.
AC   Q42682;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 1.
DT   16-JUN-2009, entry version 52.
DE   RecName: Full=Delta-aminolevulinic acid dehydratase, chloroplastic;
DE            Short=ALADH;
DE            EC=4.2.1.24;
DE   AltName: Full=Porphobilinogen synthase;
DE   Flags: Precursor;
GN   Name=HEMB; Synonyms=ALAD;
OS   Chlamydomonas reinhardtii.
OC   Eukaryota; Viridiplantae; Chlorophyta; Chlorophyceae;
OC   Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=NO-;
RX   MEDLINE=95201253; PubMed=7894023; DOI=10.1007/BF00019326;
RA   Matters G.L., Beale S.I.;
RT   "Structure and expression of the Chlamydomonas reinhardtii alad gene
RT   encoding the chlorophyll biosynthetic enzyme, delta-aminolevulinic
RT   acid dehydratase (porphobilinogen synthase).";
RL   Plant Mol. Biol. 27:607-617(1995).
CC   -!- CATALYTIC ACTIVITY: 2 5-aminolevulinate = porphobilinogen + 2
CC       H(2)O.
CC   -!- COFACTOR: Magnesium.
CC   -!- PATHWAY: Porphyrin metabolism; protoporphyrin-IX biosynthesis;
CC       coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
CC   -!- SUBUNIT: Homooctamer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the ALADH family.
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DR   EMBL; U19876; AAA79515.1; -; mRNA.
DR   PIR; S53487; S53487.
DR   HSSP; P13716; 1E51.
DR   PRIDE; Q42682; -.
DR   BRENDA; 4.2.1.24; 144.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004655; F:porphobilinogen synthase activity; IEA:EC.
DR   GO; GO:0015995; P:chlorophyll biosynthetic process; IEA:UniProtKB-KW.
DR   InterPro; IPR001731; 4pyrrol_synth_porphobiln_synth.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   Gene3D; G3DSA:3.20.20.70; Aldolase_TIM; 1.
DR   PANTHER; PTHR11458; AlaD_dehydratase; 1.
DR   Pfam; PF00490; ALAD; 1.
DR   PIRSF; PIRSF001415; Porphbilin_synth; 1.
DR   PRINTS; PR00144; DALDHYDRTASE.
DR   ProDom; PD002304; AlaD_dehydratase; 1.
DR   PROSITE; PS00169; D_ALA_DEHYDRATASE; 1.
PE   2: Evidence at transcript level;
KW   Chlorophyll biosynthesis; Chloroplast; Lyase; Magnesium; Plastid;
KW   Porphyrin biosynthesis; Transit peptide.
FT   TRANSIT       1     24       Chloroplast (Potential).
FT   CHAIN        25    390       Delta-aminolevulinic acid dehydratase,
FT                                chloroplastic.
FT                                /FTId=PRO_0000013315.
FT   REGION      172    190       Magnesium-binding (By similarity).
FT   ACT_SITE    312    312       By similarity.
SQ   SEQUENCE   390 AA;  43046 MW;  6114C4E0325A4242 CRC64;
     MQMMQRNVVG QRPVAGSRRS LVVANVAEVT RPAVSTNGKH RTGVPEGTPI VTPQDLPSRP
     RRNRRSESFR ASVREVNVSP ANFILPIFIH EESNQNVPIA SMPGINRLAY GKNVIDYVAE
     PRSYGVNQVV VFPKTPDHLK TQTAEEAFNK NGLSQRTIRL LKDSFPDLEV YTDVALDPYN
     SDGHDGIVSD AGVILNDETI EYLCRQAVSQ AEAGADVVSP SDMMDGRVGA IRRALDREGF
     TNVSIMSYTA KYASAYYGPF RDALASAPKP GQAHRRIPPN KKTYQMDPAN YREAIREAKA
     DEAEGADIMM VKPGMPYLDV VRLLRETSPL PVAVYHVSGE YAMLKAAAER GWLNEKDAVL
     EAMTCFRRAG GDLILTYYGI EASKWLAGEK
//