Reviewed, UniProtKB/Swiss-Prot Q42682 (HEM2_CHLRE)

Names and origin

Protein names

Recommended name:
    Delta-aminolevulinic acid dehydratase, chloroplastic
      Short name=ALADH
    EC=4.2.1.24
Alternative name(s):
    Porphobilinogen synthase

Gene names

Name:	HEMB
Synonyms:	ALAD

Organism

Chlamydomonas reinhardtii

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	2 5-aminolevulinate = porphobilinogen + 2 H2O.
Cofactor	Magnesium.
Pathway	Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.
Subunit structure	Homooctamer By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the ALADH family.

Ontologies

Keywords
Biological process	Chlorophyll biosynthesis Porphyrin biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	Magnesium
Molecular function	Lyase
Gene Ontology (GO)
Biological process	chlorophyll biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW porphobilinogen synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 24	24	Chloroplast Potential
	Chain	25 – 390	366	Delta-aminolevulinic acid dehydratase, chloroplastic		PRO_0000013315
Regions
	Region	172 – 190	19	Magnesium-binding By similarity
Sites
	Active site	312	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42682-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 6114C4E0325A4242	FASTA	390	43,046
	10 20 30 40 50 60 MQMMQRNVVG QRPVAGSRRS LVVANVAEVT RPAVSTNGKH RTGVPEGTPI VTPQDLPSRP 70 80 90 100 110 120 RRNRRSESFR ASVREVNVSP ANFILPIFIH EESNQNVPIA SMPGINRLAY GKNVIDYVAE 130 140 150 160 170 180 PRSYGVNQVV VFPKTPDHLK TQTAEEAFNK NGLSQRTIRL LKDSFPDLEV YTDVALDPYN 190 200 210 220 230 240 SDGHDGIVSD AGVILNDETI EYLCRQAVSQ AEAGADVVSP SDMMDGRVGA IRRALDREGF 250 260 270 280 290 300 TNVSIMSYTA KYASAYYGPF RDALASAPKP GQAHRRIPPN KKTYQMDPAN YREAIREAKA 310 320 330 340 350 360 DEAEGADIMM VKPGMPYLDV VRLLRETSPL PVAVYHVSGE YAMLKAAAER GWLNEKDAVL 370 380 390 EAMTCFRRAG GDLILTYYGI EASKWLAGEK

References

[1]

"Structure and expression of the Chlamydomonas reinhardtii alad gene encoding the chlorophyll biosynthetic enzyme, delta-aminolevulinic acid dehydratase (porphobilinogen synthase)." Matters G.L., Beale S.I. Plant Mol. Biol. 27:607-617(1995) [PubMed: 7894023] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: NO-.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U19876 mRNA. Translation: AAA79515.1.
PIR	S53487.
3D structure databases
SMR	Q42682. Positions 58-387.
ModBase	Search...
Proteomic databases
PRIDE	Q42682.
Enzyme and pathway databases
BRENDA	4.2.1.24. 144.
Family and domain databases
InterPro	IPR001731. 4pyrrol_synth_porphobiln_synth. IPR013785. Aldolase_TIM. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR11458. AlaD_dehydratase. 1 hit.
Pfam	PF00490. ALAD. 1 hit. [Graphical view]
PIRSF	PIRSF001415. Porphbilin_synth. 1 hit.
PRINTS	PR00144. DALDHYDRTASE.
PROSITE	PS00169. D_ALA_DEHYDRATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEM2_CHLRE

Accession

Primary (citable) accession number: Q42682

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	January 19, 2010
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)