ID   G3PC_CRAPL              Reviewed;         337 AA.
AC   Q42671;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   16-JUN-2009, entry version 56.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase, cytosolic;
DE            EC=1.2.1.12;
GN   Name=GAPC;
OS   Craterostigma plantagineum.
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Lamiales; Linderniaceae; Craterostigma.
OX   NCBI_TaxID=4153;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   MEDLINE=95036031; PubMed=7948905; DOI=10.1007/BF00039567;
RA   Velasco R., Salamini F., Bartels D.;
RT   "Dehydration and ABA increase mRNA levels and enzyme activity of
RT   cytosolic GAPDH in the resurrection plant Craterostigma
RT   plantagineum.";
RL   Plant Mol. Biol. 26:541-546(1994).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; X78307; CAA55116.1; -; mRNA.
DR   PIR; S42479; S42479.
DR   HSSP; P56649; 1IHX.
DR   BRENDA; 1.2.1.12; 280962.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    337       Glyceraldehyde-3-phosphate dehydrogenase,
FT                                cytosolic.
FT                                /FTId=PRO_0000145598.
FT   NP_BIND      13     14       NAD (By similarity).
FT   REGION      153    155       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      213    214       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    154    154       Nucleophile (By similarity).
FT   BINDING      35     35       NAD (By similarity).
FT   BINDING      82     82       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     184    184       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     236    236       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     318    318       NAD (By similarity).
FT   SITE        181    181       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   337 AA;  36477 MW;  BF73D81E417580E5 CRC64;
     MAKVKIGING FGRIGRLVAR VALVRDDVEL VAVNDPFITV DYMAYMFKYD TVHGQYKHHE
     LKVKDEKTLL FGDKPVAVFG LRNPEEIPWA ETGAEYVVES TGVFTEKEKA AAHLKGGAKK
     VIISAPSKDA PMFVVGVNEK TYTPDIDVVS NASCTTNCLA PLAKVIHDRF GIVEGLMTTV
     HSITATQKTV DGPSSKDWRG GRAASFNIIP SSTGAAKAVG KVLPDLNGKL TGMAFRVPTV
     DVSVVDLTVT LAKEASYDEI KAAIKEESEG KLKGILGYTE EDVVSSDFVG DSRSSIFDAK
     AGIALSKKFV KIVAWYDNEW GYSSRVVDLI RHMAAAK
//