Q42671 (G3PC_CRAPL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase, cytosolic
EC=1.2.1.12

Gene names

Name:

GAPC

Organism

Craterostigma plantagineum

Taxonomic identifier

4153 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Lamiales › Linderniaceae › Craterostigma

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro NADP binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Glyceraldehyde-3-phosphate dehydrogenase, cytosolic		PRO_0000145598
Regions
	Nucleotide binding	13 – 14	2	NAD By similarity
	Region	153 – 155	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	213 – 214	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	154	1	Nucleophile By similarity
	Binding site	35	1	NAD By similarity
	Binding site	82	1	NAD; via carbonyl oxygen By similarity
	Binding site	184	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	236	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	318	1	NAD By similarity
	Site	181	1	Activates thiol group during catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q42671 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: BF73D81E417580E5	FASTA	337	36,477
	10 20 30 40 50 60 MAKVKIGING FGRIGRLVAR VALVRDDVEL VAVNDPFITV DYMAYMFKYD TVHGQYKHHE 70 80 90 100 110 120 LKVKDEKTLL FGDKPVAVFG LRNPEEIPWA ETGAEYVVES TGVFTEKEKA AAHLKGGAKK 130 140 150 160 170 180 VIISAPSKDA PMFVVGVNEK TYTPDIDVVS NASCTTNCLA PLAKVIHDRF GIVEGLMTTV 190 200 210 220 230 240 HSITATQKTV DGPSSKDWRG GRAASFNIIP SSTGAAKAVG KVLPDLNGKL TGMAFRVPTV 250 260 270 280 290 300 DVSVVDLTVT LAKEASYDEI KAAIKEESEG KLKGILGYTE EDVVSSDFVG DSRSSIFDAK 310 320 330 AGIALSKKFV KIVAWYDNEW GYSSRVVDLI RHMAAAK

References

[1]	"Dehydration and ABA increase mRNA levels and enzyme activity of cytosolic GAPDH in the resurrection plant Craterostigma plantagineum." Velasco R., Salamini F., Bartels D. Plant Mol. Biol. 26:541-546(1994) [PubMed: 7948905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Leaf.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X78307 mRNA. Translation: CAA55116.1.
PIR	S42479.
3D structure databases
ProteinModelPortal	Q42671.
SMR	Q42671. Positions 5-333.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR020831. GlycerAld/Erythrose_P_DH. IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020828. GlycerAld_3-P_DH_NAD(P)-bd. IPR006424. Glyceraldehyde-3-P_DH_1. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PC_CRAPL

Accession

Primary (citable) accession number: Q42671

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	December 14, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program