Reviewed, UniProtKB/Swiss-Prot Q42670 (PLSC_COCNU)

Names and origin

Protein names	Recommended name:     1-acyl-sn-glycerol-3-phosphate acyltransferase       Short name=1-AGP acyltransferase       Short name=1-AGPAT     EC=2.3.1.51 Alternative name(s):     Lysophosphatidic acid acyltransferase       Short name=LPAAT
Organism	Cocos nucifera (Coconut)
Taxonomic identifier	13894 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Arecaceae › Arecoideae › Cocoseae › Attaleinae › Cocos

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts lysophosphatidic acid (LPA) into phosphatidic acid by incorporating acyl moiety at the 2 position. This enzyme shows a preference for medium-chain-length fatty acyl-coenzyme a substrates.
Catalytic activity	Acyl-CoA + 1-acyl-sn-glycerol 3-phosphate = CoA + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Domain	The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate By similarity.
Sequence similarities	Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-acylglycerol-3-phosphate O-acyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	1-acyl-sn-glycerol-3-phosphate acyltransferase		PRO_0000208185
Regions
	Transmembrane	65 – 85	21	Potential
	Transmembrane	124 – 144	21	Potential
	Transmembrane	148 – 168	21	Potential
	Motif	130 – 135	6	HXXXXD motif

Sequences

	Sequence		Length	Mass (Da)
	Q42670-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 3773CF8C03C3C0FE	FASTA	308	34,809
	10 20 30 40 50 60 MDASGASSFL RGRCLESCFK ASFGMSQPKD AAGQPSRRPA DADDFVDDDR WITVILSVVR 70 80 90 100 110 120 IAACFLSMMV TTIVWNMIML ILLPWPYARI RQGNLYGHVT GRMLMWILGN PITIEGSEFS 130 140 150 160 170 180 NTRAIYICNH ASLVDIFLIM WLIPKGTVTI AKKEIIWYPL FGQLYVLANH QRIDRSNPSA 190 200 210 220 230 240 AIESIKEVAR AVVKKNLSLI IFPEGTRSKT GRLLPFKKGF IHIALQTRLP IVPMVLTGTH 250 260 270 280 290 300 LAWRKNSLRV RPAPITVKYF SPIKTDDWEE EKINHYVEMI HALYVDHLPE SQKPLVSKGR DASGRSNS

References

[1]

"Cloning of a coconut endosperm cDNA encoding a 1-acyl-sn-glycerol-3-phosphate acyltransferase that accepts medium-chain-length substrates." Knutzon D.S., Lardizabal K.D., Nelsen J.S., Bleibaum J.L., Davies H.M., Metz J.G. Plant Physiol. 109:999-1006(1995) [PubMed: 8552723] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Endosperm.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U29657 mRNA. Translation: AAC49119.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.3.1.51. 118852.
Family and domain databases
InterPro	IPR002123. Acyltransferase. IPR004552. AGP_acyltrans. [Graphical view]
Pfam	PF01553. Acyltransferase. 1 hit. [Graphical view]
SMART	SM00563. PlsC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00530. AGP_acyltrn. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PLSC_COCNU

Accession

Primary (citable) accession number: Q42670

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)