Reviewed, UniProtKB/Swiss-Prot Q42669 (ACOC_CUCMC)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase
      Short name=Aconitase
    EC=4.2.1.3
Alternative name(s):
    Citrate hydro-lyase

Gene names

Name:

ACO

Organism

Cucumis melo var. conomon (Oriental pickling melon)

Taxonomic identifier

3657 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Cucurbitales › Cucurbitaceae › Cucumis

Protein attributes

Sequence length	764 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; glyoxylate and dicarboxylate metabolism.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Glyoxylate bypass
Cellular component	Cytoplasm
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 764	›764	Aconitate hydratase		PRO_0000076653
Sites
	Metal binding	307	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	372	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	375	1	Iron-sulfur (4Fe-4S) By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	Q42669-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: E4A9B011FC6922F0	FASTA	764	83,274
	10 20 30 40 50 60 HEAKTENAVQ ANMELEFKRN RERFGFLKWG SSAFHNMLVV PPGSGIVHQV NLEYLGRVVF 70 80 90 100 110 120 NTNGLLYPDS VVGTDSHTTM IDGLGVAGWG VGGIEAEAAM LGQPMSMVLP GVVGFKLVGK 130 140 150 160 170 180 LRNGVTATDL VLTVTQMLRK HGVVGKFVEF YGEGMGELSL ADRATIANMS PEYGATMGFF 190 200 210 220 230 240 PVDHVTLQYL KLTGRKDETI SMIESYLLAN KMFVDYSEPQ VERVYSSHIE LNLSDVEPCI 250 260 270 280 290 300 SGPKRPHDRV PLKEMKADWH ACLDNRVGFK GFAIPKEAQV KVAEFNFHGS PAQLRHGDVV 310 320 330 340 350 360 IAAITSCTNT SSSVMLGAAL VAKKACELGL EVKPWIKTVL LQALGVVTKY LAKSGLQKYL 370 380 390 400 410 420 NQLGFNIVGY GCTTCIGNSG DIDESVASAI TGNDIVAAAV LSGNRNFEGR VHPLTRANYL 430 440 450 460 470 480 ASPPLVVAYA LAGTVDIDFE SEPIGVGKDG KKVFFRDIWP TSEEVAVVVN SNVLPDMFRA 490 500 510 520 530 540 TYQAITEGNA TWNLLSVPEG TLYSWDPTST YIHEPPYFKD MSMSPPGPHG VKNAYCLLNF 550 560 570 580 590 600 GDSITTDHIS PAGSIHKDSP AAKYLLERGV DRRDFNSYGV AVVMMRLWHV HFANIRIVNK 610 620 630 640 650 660 LLKGEVGPKT IHIPSREKLS VFDAAMRYKS EGQDTIILAG AEYGIGSSRD WAAKGPMLLG 670 680 690 700 710 720 VKAVIAKSFE RIHRSNLVGM GIIPLCFKAG EDADSLGLTG HERFTIDLPS NVGEIRPGQD 730 740 750 760 VAVVTDTGKS FSCILRFDTE VELAYFDHGG ILQYVIRNLI HSKH

References

[1]

"Structure, genomic organization, and expression of the Arabidopsis thaliana aconitase gene. Plant aconitase show significant homology with mammalian iron-responsive element-binding protein." Peyret P., Perez P., Alric M. J. Biol. Chem. 270:8131-8137(1995) [PubMed: 7713917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Cantaloupe. Tissue: Fruit.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X82840 mRNA. Translation: CAA58047.1.
PIR	S49849.
3D structure databases
HSSP	HSSP built from PDB template 1AMJ based on UniProtKB P20004.
ModBase	Search...
Enzyme and pathway databases
BRENDA	4.2.1.3. 289996.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACOC_CUCMC

Accession

Primary (citable) accession number: Q42669

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)