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Protein

Alpha-galactosidase

Gene
N/A
Organism
Coffea arabica (Arabian coffee)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Preferentially cleaves alpha-1,3 and alpha-1,4 glycoside linkages. Involved in the hydrolysis of the galactomannan, it splits alpha-linked galactose moieties. It is particularly suitable for the hydrolysis of guar gum to a gum with improved gelling properties. Can cleave terminal alpha-1,3-linked galactose residues responsible for blood group B specificity from the surface of erythrocytes thereby converting these cells serologically to group O.

Catalytic activityi

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei145NucleophileBy similarity1
Active sitei200Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Protein family/group databases

CAZyiGH27. Glycoside Hydrolase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-galactosidase (EC:3.2.1.22)
Alternative name(s):
Alpha-D-galactoside galactohydrolase
Melibiase
OrganismiCoffea arabica (Arabian coffee)
Taxonomic identifieri13443 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsGentianalesRubiaceaeIxoroideaeGardenieae complexBertiereae - Coffeeae cladeCoffeeaeCoffea

Pathology & Biotechi

Biotechnological usei

Used to convert human blood group antigens of type B into type O, the universal donor type.3 Publications

Chemistry databases

ChEMBLiCHEMBL5217.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 151 PublicationAdd BLAST15
ChainiPRO_000000100116 – 378Alpha-galactosidaseAdd BLAST363

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi36 ↔ 68By similarity
Disulfide bondi116 ↔ 147By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiQ42656.

Interactioni

Chemistry databases

BindingDBiQ42656.

Structurei

3D structure databases

ProteinModelPortaliQ42656.
SMRiQ42656.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni178 – 182Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the glycosyl hydrolase 27 family.Curated

Keywords - Domaini

Signal

Family and domain databases

CDDicd14792. GH27. 1 hit.
Gene3Di3.20.20.70. 2 hits.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR002241. Glyco_hydro_27.
IPR000111. Glyco_hydro_27/36_CS.
IPR017853. Glycoside_hydrolase_SF.
IPR035373. Melibiase/NAGA_C.
PfamiView protein in Pfam
PF16499. Melibiase_2. 1 hit.
PF17450. Melibiase_2_C. 1 hit.
PRINTSiPR00740. GLHYDRLASE27.
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiView protein in PROSITE
PS00512. ALPHA_GALACTOSIDASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q42656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKSPGTEDY TRRSLLANGL GLTPPMGWNS WNHFRCNLDE KLIRETADAM
60 70 80 90 100
VSKGLAALGY KYINLDDCWA ELNRDSQGNL VPKGSTFPSG IKALADYVHS
110 120 130 140 150
KGLKLGIYSD AGTQTCSKTM PGSLGHEEQD AKTFASWGVD YLKYDNCNNN
160 170 180 190 200
NISPKERYPI MSKALLNSGR SIFFSLCEWG EEDPATWAKE VGNSWRTTGD
210 220 230 240 250
IDDSWSSMTS RADMNDKWAS YAGPGGWNDP DMLEVGNGGM TTTEYRSHFS
260 270 280 290 300
IWALAKAPLL IGCDIRSMDG ATFQLLSNAE VIAVNQDKLG VQGNKVKTYG
310 320 330 340 350
DLEVWAGPLS GKRVAVALWN RGSSTATITA YWSDVGLPST AVVNARDLWA
360 370
HSTEKSVKGQ ISAAVDAHDS KMYVLTPQ
Length:378
Mass (Da):41,310
Last modified:November 1, 1997 - v1
Checksum:i9FC7610BFD760AE3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27992 mRNA. Translation: AAA33022.1.
PIRiT50781.

Similar proteinsi

Entry informationi

Entry nameiAGAL_COFAR
AccessioniPrimary (citable) accession number: Q42656
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: August 30, 2017
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families